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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Molecular Biology 2019Quote: ... contained 40 µM human Flag-tagged ubiquitin (Fisher Scientific U-120), 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant Cyclin A/CDK2 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).
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bioRxiv - Molecular Biology 2023Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant cyclin B/CDK1 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Human recombinant bFGF and EGF were purchased from Invitrogen/Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human recombinant EGF (Thermo Fisher) at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg/mL human recombinant insulin (Thermo Fisher Scientific), 20 μg/mL ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 ng/ml human recombinant FGF8 (Gibco, PHG0184). The medium was refreshed daily for 7–10 days as the cells aggregated to form spheroids.
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng/mL human recombinant FGF (Gibco, #AA10-155), 10 ng/mL heparin sulfate (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.01 mg/ml human recombinant insulin (Life Technologies). T-47D cells were cultured in RPMI-1640 Medium (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: Recombinant human Dynactin was expressed using Expi293F cells (Invitrogen) and Expi293 expression medium (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1pM Insulin Human Recombinant Zinc (Life Technologies 12585-014). For generating steatosis cells were supplemented oleic acid-albumin (Sigma-O3008 ...
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bioRxiv - Immunology 2020Quote: ... or 20 ng/ml recombinant human IL4 (Life Technologies), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... MA) and recombinant human insulin was obtained from Gibco.
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bioRxiv - Cancer Biology 2021Quote: ... Human recombinant insulin (10 μg ml−1, Gibco #12585014) was additionally included in the culture media for MCF7 ...
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bioRxiv - Microbiology 2022Quote: ... and 50 IU/mL recombinant human IL-2 (Gibco). IL-2 was also supplemented to each culture for the maintenance of the cells.
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/mL recombinant human EGF (#PHG0313, Gibco, USA), 20 % Knockout Serum Replacement (#10828028 ...
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bioRxiv - Physiology 2023Quote: ... hiPSCs were cultured on recombinant human vitronectin (rhVTN, ThermoFisher) in E8 Flex medium (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... 10 ng/mL recombinant human epidermal growth factor (Invitrogen), 0.1 μM Dibenzazepine (DBZ ...
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bioRxiv - Immunology 2023Quote: ... 294.4 ng/l human recombinant EGF (Gibco, 10450-013) and 300 µM of CaCl2 (Kanto Chemicals ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg/mL human recombinant insulin (Thermo Fisher Scientific), 1mM sodium pyruvate (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... 50 ng/ml human recombinant EGF (10450-013; Gibco), 3.0% Noggin/R- Spondin-conditioned media ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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bioRxiv - Cancer Biology 2022Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Developmental Biology 2023Quote: ... on recombinant human Vitronectin (A14700, Thermo Fisher / Life Technologies)-coated 6-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg/mL recombinant human insulin (Thermo Fisher Scientific), 0.55 µg/mL human transferrin (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...