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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... coli (C737303; Invitrogen) cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163 ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α (Invitrogen). GentR colonies were then screened by PCR for the full insert ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α (Invitrogen). GentR colonies were then screened by PCR for both inserts ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α (Invitrogen). GentR colonies were then screened by PCR for the NTD insert ...
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bioRxiv - Microbiology 2024Quote: ... coli strain (Invitrogen) was used for cloning and routinely grown at 37°C (30°C for protein production ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli cells (ThermoFisher) using standard protocols ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Biochemistry 2023Quote: ... The molecular weight of the recombinant proteins was estimated using Invitrogen™ BenchMark™ Pre-stained Protein Ladder (Fisher Scientific, Waltham, Massachusetts, USA). Eluted proteins were concentrated using Vivaspin® 10,000 MWCO centrifugal filter units (Sartorius ...
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bioRxiv - Microbiology 2023Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Genomics 2020Quote: ... and gel imager (E-Gel Safe Imager connected to E-Gel iBase, Invitrogen), according to the recommendations provided by the manufacturer ...
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bioRxiv - Bioengineering 2021Quote: Our RGD-containing elastin-like proteins (ELP) are expressed in BL21 (DE3) pLysS Escherichia coli (Invitrogen, 1931008) under control of the T7 promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...
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bioRxiv - Plant Biology 2022Quote: For recombinant protein expression in Pichia pastoris KM71H (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs encoding SCPSs ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant Streptococcus pyogenes Cas9 protein (SpCas9; Synthego) using Lipofectamine CRISPRMAX Cas9 Transfection reagent (CMAX00001; Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were labelled by conjugation with either Alexa Fluor 488 or 647 following maleimide chemistry (Invitrogen), as in (Picas et al. ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: Recombinant PLD3 and PLD4 proteins were expressed in Expi293F cell line according to the manufacturer’s instructions (ThermoFisher). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Developmental Biology 2022Quote: ... donated foetal tissue stored in Hibernate-E medium (Hib-E, Thermo Fisher Scientific A1247601) was collected from local maternity hospital soon after passing ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Cell Biology 2023Quote: ... 1:1000 secondary antibody Alexa488 for E protein labeling (A-21121, goat anti-mouse, Thermo Fisher, Waltham, MA, USA), and 1:1000 Hoechst-33342 ...
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bioRxiv - Immunology 2022Quote: ... E-cadherin (Invitrogen, PA5- 85088), EpCAM (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cyclin E (Invitrogen, MA5-32358), GAPDH (GeneTex ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... William’s E Medium (Gibco, A1217601) supplemented with Primary Hepatocyte Maintenance Supplements (Gibco ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... E-selectin (Invitrogen #MA1-06506), ZO-1 (Invitrogen #MA3-39100-A488) ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin (ThermoFisher, PA5-32178) 1:5000 ...
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bioRxiv - Biophysics 2021Quote: ... Coli TOP10 cells (Invitrogen) were used for plasmid propagation and isolation ...
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bioRxiv - Biophysics 2021Quote: ... Coli DH10Bac cells (Invitrogen). Recombinant baculoviruses were produced and amplified in Sf9 cells ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells (Thermo Fisher). Cells were grown in 2xYT medium with appropriate antibiotics at 37°C with shaking until OD600 reached ∼ 0.4 – 0.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... coli (Thermo Fisher Scientific) were transformed with the ligation product ...
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bioRxiv - Microbiology 2020Quote: ... coli cells (Invitrogen, UK). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Thermo Fisher #C737303), and several colonies were picked up and sent for sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Thermo Fisher #C737303), and positive clones were screen by colony PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (Thermo Fisher Scientific). Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific) ...