Labshake search
Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant Bovine FCGRT & B2M Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Recombinant proteins were coated in PBS buffer (Gibco) at the concentration of 1.2 mg/ml of protein per well ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
-
bioRxiv - Bioengineering 2022Quote: ... and BMP4 recombinant protein (Gibco; 5 ng/mL). The media was half-changed every day during the differentiation process ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Recombinant magnetic protein G beads (Invitrogen, MA, USA) were added for 1 hour at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen, PHG0313), 1.1 μM hydrocortisone (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... and qRT-PCR of B2m (NM_009735.3, TaqMan® assays, Thermo Fisher Scientific) was performed using TaqMan PCR Master Mix (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... HA-tagged polyQ and Myc-tagged polyA proteins were enriched using Pierce™ Anti-HA Agarose (Thermo Fisher Scientific) and Myc-Trap®_A Kit (Chromotek ...
-
bioRxiv - Biophysics 2023Quote: ... the channel was incubated in biotinylated Bovine Serum Albumin (Thermo Scientific, cat no. 29130) by flushing 0.5 mL of a 1 mg/mL biotinylated BSA solution in buffer I ...
-
bioRxiv - Cancer Biology 2023Quote: ... bovine pituitary extract and human recombinant epidermal growth factor (Gibco, 37000-015)) and primary gingival keratinocyte (Dermal cell basal medium (ATCC ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Recombinant protein was extracted using BugBuster Protein Extraction Reagent (Thermo Scientific, USA) and purified using cOmplete His-Tag Purification Resin (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were eluted by incubation with HA peptide (ThermoFisher) for 2 × 15 minutes at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: To localize GFP-tagged fusion proteins with BODIPY Red (Molecular Probes), transfected cells were grown overnight (16-20h ...
-
bioRxiv - Cell Biology 2020Quote: ... To localize GFP-tagged fusion proteins with MitoTracker Red (Molecular Probes), after 16h transfection ...
-
bioRxiv - Microbiology 2021Quote: ... for FLAG® (DYKDDDDK)-tagged proteins in iBind Western System (Invitrogen). Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
-
bioRxiv - Developmental Biology 2019Quote: ... tagged proteins were eluted and quantified by BCA (Pierce, ThermoFisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... Tagged proteins were visualized using chemiluminescent reagents (Thermo Fisher Scientific 34579).
-
bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
-
bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
-
bioRxiv - Cancer Biology 2019Quote: ... bait GST-tagged EIF4E or 6xHis-tagged CELF1 proteins (1 μM) were initially bound to glutathione-agarose beads (ThermoFisher Scientific) or Ni2+ beads (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum [HI-FBS] (Cat#SH30071.03, Thermo Scientific), penicillin [100 IU/ml]-streptomycin [100 ug/ml] (Quality Biological ...
-
Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: His-tagged P-Rex2 was expressed in Sf9 cells for 2 days following baculovirus infection (Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
-
bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were detected with Neutravidin-HRP (Thermofisher, A2664, 1µg/ml). D-biotin was from Fisher (BP-232-1).
-
bioRxiv - Neuroscience 2021Quote: ... Biotinylated proteins were precipitated with streptavidin-agarose beads (Thermo Fisher Scientific) at 4°C overnight ...
-
bioRxiv - Microbiology 2021Quote: ... For the detection of biotinylated proteins horseradish peroxidase-conjugated streptavidin (Invitrogen) was used at a 1:5000 dilution ...
-
bioRxiv - Biophysics 2021Quote: ... biotinylated Pierce™ protein A (#29989, Thermo Fisher Scientific, Waltham, USA) or custom biotinylated aldolase (#28403842 ...
-
bioRxiv - Immunology 2021Quote: S1 protein was biotinylated according to manufacturer’s instructions (EZ-link, Thermofisher), and was conjugated to a U-Plex MSD linker (Mesoscale Diagnostics) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Biotinylated proteins were purified with streptavidin beads (Thermo Fisher Scientific, #88817) and eluted by boiling in Laemmli sample buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were allowed to bind to streptavidin sepharose (Thermo Fisher) for 1 hr at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were enriched with MyOne T1 streptavidin beads (Thermo Fisher) after 2 h of incubation at 4 °C with rotation ...
-
bioRxiv - Molecular Biology 2022Quote: ... biotinylated proteins were visualized using a streptavidin-alexa488 conjugate (Invitrogen, #S32354). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... biotinylated proteins were captured by streptavidin magnetic beads (88817; Thermofisher Scientific) in 1.5 mL Eppendoft LoBind tubes using 83uL beads per 1mg of protein in a 500 µL RIPA lysis buffer (RLB)(50LmM Tris ...
-
bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were identified using HRP-conjugated streptavidin (Invitrogen, 1:10000). After extensive washing in PBS containing 0.1% (v/v ...
-
bioRxiv - Biochemistry 2023Quote: ... Biotinylated proteins were identified with Alexa Fluor 647-conjugated streptavidin (Invitrogen). Primary antibodies were incubated for 1 hr (1:1000 dilution in PBS with 1% BSA and 0.3% Triton X-100 ...
-
Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2023Quote: ... biotinylated proteins were captured by streptavidin magnetic beads (88817; Thermofisher Scientific) in 1.5 mL Eppendorf LoBind tubes using 83uL beads per 1mg of protein (here 200 µg of protein with 16.6 µL of beads ...
-
bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
-
bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...