Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Atlantic Salmon il4 13a Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
-
bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
-
bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... to the tube was added a solution of 0.269 mg of P58 mixed 5:1 by volume with salmon sperm DNA (Invitrogen), followed by 3 mL of 39.52% polyethylene glycol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells in each well were transiently transfected using 100 ng V2R and 900 ng sheared salmon sperm DNA (Invitrogen) and 1:3 25 kDa linear Polyethylenimine (Polysciences ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... were transfected with a fixed final amount of pre-mixed biosensor-encoding DNA (0.57 μg, adjusted with salmon sperm DNA; Invitrogen) and human receptor DNA ...
-
bioRxiv - Genomics 2020Quote: ... The samples were genotyped using a 200K Affymetrix Axiom® myDesign Custom SNP Array developed by the EPIC4 coho salmon genome consortium (http://www.epic-4.org) and built by ThermoFisher Scientific (San Diego ...
-
bioRxiv - Genomics 2020Quote: ... the nuclear extract was pre-incubated for 20 min at 30°C with the nonspecific Salmon Sperm DNA (Invitrogen): 1 µg of DNA per 1.5 µl of the nuclear extract (approximately 5 µg ...
-
bioRxiv - Genetics 2019Quote: ... A 7 μl aliquot of the pooled library was aliquoted and mixed with 0.8 μl of a 10 μg/μl UltraPure Salmon Sperm DNA Solution (Invitrogen) in a PCR-tube ...
-
bioRxiv - Biochemistry 2023Quote: ... AAV libraries were first reacted with 1 nmol biotinylated ssDNA oligos (sequences are given in Supplemental Table 3) in PBS supplemented with 0.05 % v/v salmon sperm DNA (Invitrogen), 1 mM MgCl2 ...
-
bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
-
Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant human TGF-β1 (Life Technologies) was prepared in sterile water with 0.1% BSA following the manufacturer’s instructions and diluted in 50:50 media.
-
bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
-
bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
-
bioRxiv - Genomics 2019Quote: ... 50ng/mL recombinant murine EGF (Gibco), and 100ug/mL recombinant human Noggin (Peprotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
-
bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
-
bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
-
bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
-
bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
-
bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
-
bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
-
bioRxiv - Immunology 2023Quote: ... the microtiter plates were washed 5 × with PBS-T and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of both bat and human IgG antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
-
bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
-
bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
-
bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
-
bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
-
bioRxiv - Genetics 2021Quote: ... followed by the addition of 40 μl of denatured carrier DNA solution (salmon sperm ssDNA, 10 mg ml-1; Invitrogen). Aliquots of 50 μl of these competent cells were used immediately for transformation (for maximum efficiency ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were then pre-hybridized for 2 hr at 50°C in SCC buffer containing 50% deionized formamide and 40 μg/mL salmon DNA (Thermofisher) and then incubated with sense or antisense probes at 75°C for 10 min ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The ADP-ribosylation reactions contained 0.2 μM purified PARP-1 or PARP-3 and 100 ng/μL of sheared salmon sperm DNA (Invitrogen, AM9680). Purified proteins were mixed with the indicated amounts of NAD+ in ADP-ribosylation Buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Each plate included four DNA standards diluted from known concentrations of synthesized gene fragments (IDT gBlocks) with the addition of nonspecific salmon sperm DNA (ThermoFisher). All plates included control reactions with water in place of the cDNA template (no-template controls ...
-
bioRxiv - Developmental Biology 2019Quote: ... and hybridization was added more yeast RNA (100 μg/mL) and Salmon Sperm DNA (100 μg/mL) (Thermo Fisher Scientific). For post-hybridization ...
-
bioRxiv - Developmental Biology 2020Quote: ... Discs were transferred to hybridization buffer (10% w/v dextran sulfate, 4X SSC, 0.01% w/v salmon sperm ssDNA (Invitrogen 15632), 1% v/v vanadyl ribonucleoside (NEB S14025) ...
-
bioRxiv - Microbiology 2021Quote: ... which had been washed three times in lysis buffer and blocked with 100 mg/mL sheared salmon sperm DNA (Ambion), were added to chromatin-antibody mixture and incubated at 4°C for 4 hours ...
-
bioRxiv - Pathology 2021Quote: ... The DNA was then spiked at a final concentration of 1.33 ng μl-1 in salmon sperm gDNA (SSD, Thermo Fisher) at 10 ng μl-1 in artificial seawater (ASW ...
-
bioRxiv - Cell Biology 2019Quote: ... For iPALM experiments 1µg of DNA and 2µl of sheared salmon sperm DNA were mixed together in 15µl of Opti-MEM (Thermofisher scientific, 31985062), and kept on ice for 15 min ...
-
bioRxiv - Cell Biology 2021Quote: To prepare the probe for hybridisation approximately 200-600ng of labelled probe DNA was ethanol precipitated with the 5 µg of sheared salmon sperm DNA and 5 µg of mouse or human Cot1 DNA (both from Invitrogen) and resuspended in hybridisation buffer (50% formamide ...
-
bioRxiv - Neuroscience 2022Quote: ... plates were washed twice with TBST and blocked with 5% BSA + 0.1 mg/ml salmon sperm DNA (ThermoFisher, cat# 15632011), 100ul/well ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 100 μL of this mixture was added to 64 μL DNA cocktail containing 10 μL of boiled salmon sperm DNA (ThermoFisher) per transformation ...