Labshake search
Citations for Thermo Fisher :
51 - 100 of 7801 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Retrovirus containing a control shRNA (firefly luciferase) or the Ppargc1b shRNA were produced by 293T cells transfected using Lipofectamine 2000 (Invitrogen). After 48 hours ...
-
bioRxiv - Cell Biology 2024Quote: ... either 3.6 µg of scramble shRNA or MAP6 shRNA were transfected into hippocampal neurons using Lipofectamine 2000 transfection reagent (Invitrogen, 11668030). Neurons were then collected for protein analysis using western blot.
-
bioRxiv - Cancer Biology 2020Quote: For depletion of RB1CC1 virus particles were generated in HEK 293-T cells transfected with the control vector pGIPZ or the respective shRNA plasmid (KD1: RHS4430-101100096 or KD2: RHS4430-101103887) (Thermo Scientific Open Biosystems), the gag/pol plasmid pCMV-dr8.91 and the VSV-g envelope plasmid pMD2.G (Addgene ...
-
bioRxiv - Cancer Biology 2020Quote: ... All siRNAs and shRNAs were from Thermo Fisher Scientific (Carlsbad ...
-
bioRxiv - Neuroscience 2019Quote: ... and shRNA using Lipofectamine 2000 (Invitrogen, CA, USA). Lentiviral particles collected from culture medium were isolated and concentrated according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... and pLKO.1 shRNA using Lipofectamine 3000 (Invitrogen). Cells were incubated with viral supernatant containing polybrene (8 mg/mL) ...
-
bioRxiv - Cell Biology 2023Quote: ... and the target shRNA using Lipofectamine 2000 (Invitrogen). The following day ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were either transduced with lentiviral particles carrying a control shRNA or a specific shRNA against RTP801 or transfected with lipofectamine 2000 (Thermo Fisher Scientific) with pCMS vectors expressing eGFP (donated by Dr ...
-
bioRxiv - Microbiology 2021Quote: ... and sgRNA or shRNA using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with pLKO.1 puro vector constructs expressing shRNA against mouse Aldh1a1 or Alhd1a3 and non-silencing control shRNA using Lipofectamine 2000 Transfection Reagent (Thermo Scientific, Waltham, MA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... cultured hippocampal neurons treated with adenoviruses expressing either βII-spectrin shRNA or scrambled (control) shRNA were lysed on ice using the RIPA buffer (Thermo Fisher Scientific, 89900) complemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... shRNA was synthesized using the T7 MegaShortScript kit (Invitrogen, AM1354) with an incubation time of 6 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... shRNA/siRNA transfection was carried out with Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2020Quote: ... The corresponding direct and reverse oligos for every shRNA (Invitrogen; Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Non-silencing shCTRL = RHS4743) lentiviral shRNAs were purchased from ThermoFisher Scientific (Thermo ...
-
bioRxiv - Cell Biology 2023Quote: ... Anti-rat: donkey anti-rat Alexa 647 (Invitrogen). The stained cells were mounted with fluoro-Gel mounting medium containing 4’ ...
-
bioRxiv - Neuroscience 2023Quote: ... Cellular and membrane samples (50µg/subject) were then subjected to SDS-PAGE and Western Blot protocols using TRPV1 primary antibody (ThermoFisher PA1-29421; 1:1000). Primary antibodies for Beta-actin (ThermoFisher PA1-183 ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmids were stably transfected into Fischer Rat Thyroid (FRT) cells in 6-well plates using lipofectamine LTX (Thermo Fisher) and OptiMEM (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Rat cortical neurons from Sprague Dawley rats (Gibco, A36511) were seeded at a density of 1000 cells/mm2 ...
-
bioRxiv - Neuroscience 2024Quote: ... rat hippocampal neurons from Sprague Dawley rats (Gibco, A36513) were plated at a density of 1000 cells/mm2 in the three remaining nodes of each microfluidic interface ...
-
bioRxiv - Neuroscience 2024Quote: ... Rat cortical neurons from Sprague Dawley rats (Gibco, A36511) were plated at a density of 1000 cells/mm2 ...
-
bioRxiv - Immunology 2020Quote: ... and sgRNA or shRNA lentiviral construct using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
-
bioRxiv - Neuroscience 2022Quote: ... and scrambled shRNAs were transfected into the cells using Lipofectamine (GIBCO). Seventy-two hours after transfection ...
-
bioRxiv - Neuroscience 2021Quote: ... Lentiviral shRNAs against mouse ptprt were constructed in pLKO.1 (Invitrogen) and transfected with pMD2g and psPAX into HEK293T cells for viral package ...
-
bioRxiv - Microbiology 2020Quote: ... and NCBP1 were selected from the GIPZ Lentiviral shRNA library (ThermoFisher, sh-eIF4E2 ...
-
bioRxiv - Cancer Biology 2020Quote: Lentiviral shRNA constructs were obtained from Open Biosystem (Thermo Fisher Scientific). Targeting sequences were cut from the pGIPZ constructs using MIuI and XhoI sites and cloned into pInducer10 using the same sites ...
-
bioRxiv - Developmental Biology 2022Quote: ... Concentrations of shRNA was measured with a NanoDrop One (Thermo fisher).
-
bioRxiv - Developmental Biology 2023Quote: ... HaCaT SAM and shRNA cells were done using Lipofectamine 2000 (Invitrogen). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... rat (Thermo Fisher), and human AB (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... rat (Thermo Fisher), and human (AB serum ...
-
bioRxiv - Neuroscience 2019Quote: ... rat (Thermo Fisher Scientific Cat# A-21094 ...
-
bioRxiv - Immunology 2022Quote: ... rat (Thermo Fisher), and human (AB serum ...
-
bioRxiv - Immunology 2020Quote: ... rat (Thermo Fisher), and human AB (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-rat (ThermoFisher), anti-goat (ThermoFisher) ...
-
bioRxiv - Microbiology 2023Quote: ... Rat Tail (Gibco). One tube of cryopreserved cells (∼500,000 ...
-
bioRxiv - Microbiology 2023Quote: ... rat (Thermo Fisher), and human (AB serum ...
-
bioRxiv - Neuroscience 2020Quote: ... Certified scramble shRNA (ACTACCGTTGTTATAGGTG) was also used as a negative control (Invitrogen). All plasmids used in this study were purified by NucleoBond Xtra purification kit (MACHEREY-NAGEL) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells with integrated shRNA were selected with 300μg/ml Hygromycin B (Invitrogen). The GFP expressing cells were isolated by FACS sorting (BD FACSAria Fusion).
-
bioRxiv - Molecular Biology 2022Quote: ... Patz1 shRNA sequences were designed using BLOCK-iT RNAi designer tool (Invitrogen). Sense and antisense oligos were annealed and cloned into pENTR/U6 plasmid (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... and the Ido1 shRNA using Lipofectamine 2000 (ThermoFisher Scientific, Cat. No. 11668027). Cells were transfected in virus collection medium (DMEM supplemented with 1% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... while pLL3.7-shRNA infected cells were selected with 2μg/ml puromycin (Gibco). The overexpression and knockdown efficiencies were validated with WB and qRT-PCR ...
-
bioRxiv - Cancer Biology 2023Quote: The seed shRNA sequences were designed by BLOCK-iT RNAi Designer (ThermoFisher) and cloned into lentiviral expressing vector pTY-U6-Pgk-Puro ...
-
bioRxiv - Neuroscience 2022Quote: ... 25 ng of plasmids containing sgRNAs for respective genes were transfected into rat primary neurons on DIV 4 using Lipofectamine 2000 (ThermoFisher, #11668027) along with 50 ng of TDP43(WT)-EGFP or mApple then survival was measured over the course of 10 days ...
-
bioRxiv - Microbiology 2020Quote: Individual shRNAs were designed using the BLOC-iTTM RNAi Designer (Thermo Fisher Scientific). The specificity was tested by BLAST (NCBI) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the expression of shRNA was induced by addition of doxycycline (Thermo Fisher Scientific). To this end ...
-
bioRxiv - Molecular Biology 2019Quote: ... These shRNA sequences were generated using an open-access algorithm (RNAi designer, ThermoFisher) and were as follows ...
-
bioRxiv - Biochemistry 2020Quote: ... Adenoviruses expressing these shRNAs were established as per the manufacturer’s instructions (Life Technologies) and as previously reported 50 ...
-
bioRxiv - Neuroscience 2021Quote: ... shTTL2 and control shRNA (packaging vectors pLP1, pLP2, and pLP-VSV-G, Thermofisher) were produced by co-transfection with the psPAX2 and pCMV-VSV-G helper plasmids ...
-
bioRxiv - Cell Biology 2022Quote: K8 expression was silenced in HeLa cells using a stable shRNA (Thermo Scientific) transduced by lentiviral vectors ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cell lines were transfected with shRNA constructs using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and 48 h post-transfection selected with 4 µg/ml puromycin (Invitrogen) ...