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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Rat Insulin Like Growth Factor Binding Protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... DRG neurons were plated on laminin pre-coated 35 mm culture dishes (2,000 cells per dish) in 50 ng/ml nerve growth factor/neurobasal media (Thermo Fisher), and used for calcium imaging 12-24 hr after plating ...
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bioRxiv - Bioengineering 2021Quote: ... Media was changed every 12 hours for the first 3 days with fresh hESC medium with basic fibroblast growth factor (bFGF) (Invitrogen) and ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... enzymatically-dissociated neurospheres were plated on poly-D-lysine/Laminin (1 µg/cm2) coated glass coverslips in a medium without growth factors and heparin but containing 2% fetal bovine serum (Invitrogen). After 4 days ...
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bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... and induced for differentiation in serum-containing medium (1% FBS in DME) or in Neurobasal medium without EGF and FGF growth factors but with B27 (12587-010, Invitrogen). Differentiated cells or control GSCs were collected for immunoblot analyses or fixed with PFA for immunofluorescent staining at the indicated days as described above.
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bioRxiv - Systems Biology 2020Quote: ... Levels of circulating cytokines and growth factors were measured in plasma using a 63-plex Luminex antibody-conjugated bead capture assay (eBiosciences/Affymetrix). Metabolic hormones were measured using MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay (HMHEMAG-34K ...
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bioRxiv - Bioengineering 2019Quote: ... or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50%; R-spondin 10%; Noggin 5%) and the following growth factors: B12 1X (Invitrogen), Nicotinamide 10mM (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... in mammosphere medium (serum-free MEM supplemented with 20 ng/mL of bFGF [Gibco], 20 ng/mL epidermal growth factor [Gibco], B27 1x [Gibco] ...
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bioRxiv - Cancer Biology 2021Quote: ... in mammosphere medium (serum-free MEM supplemented with 20 ng/mL of bFGF [Gibco], 20 ng/mL epidermal growth factor [Gibco] ...
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bioRxiv - Cancer Biology 2020Quote: ... CPA and CPD cells were cultured in Keratinocyte-SFM supplemented with 2.5 μg prequalified human Epidermal Growth Factor 1-53 (EGF) (Life technologies), 25 mg Bovine Pituitary Extract (BPE ...
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bioRxiv - Cell Biology 2023Quote: ... E8 supplement ThermoFisher-A1517001) on tissue-culture dishes coated with 10 µg/cm2 of reduced Growth Factor Basement Membrane matrix (Geltrex, ThermoFisher A1413202 resuspended in basal medium DMEM/F12 Thermo Fisher 21331020) ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... PDO was cultured in Nunclon™ Delta Surface (Thermoscientific) on Geltrex dome (Reduced growth factor basement membrane, hESV qualified, Gibco) suspended in PaTOM growth media (Huang et al. ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Developmental Biology 2024Quote: ... and bone marrow derived iPSCs (ACS-5003, ATCC) were cultured on ESC-grade reduced growth factor basement membrane matrix (Geltrex, Gibco) in NSC maintenance medium (Xcell Science ...
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bioRxiv - Developmental Biology 2024Quote: iPSC lines were plated onto glutaraldehyde-activated glass coverslips coated with ESC-grade reduced growth factor basement membrane matrix (Geltrex, Gibco). Neuronal cultures were plated onto glutaraldehyde-activated glass coverslips coated with appropriate substrates as mentioned above ...
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bioRxiv - Cell Biology 2023Quote: ... RWPE-1 cells were cultured in keratinocyte serum-free media supplemented with 25 mg of bovine pituitary extract and 2.5 µg epidermal growth factor (ThermoFisher, 17005042). All cells were maintained at 37°C at 5% CO2 for <15 passages ...
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bioRxiv - Immunology 2021Quote: ... IL1β and IL6 were quantified using a specific rat enzyme-linked immunosorbent assay (ELISA, Invitrogen, Thermo Scientific) following the manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... IL1β and IL6 were quantified using a specific rat enzyme-linked immunosorbent assay (ELISA, Invitrogen, Thermo Scientific) following the manufacturer protocol.
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bioRxiv - Microbiology 2019Quote: ... Caspase-like activity was measure with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen), a nucleic acid-binding dye that harbors the caspase-3/7 cleavage sequence ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted from sorted FOXL2+ granulosa-like cells using the Arcturus PicoPure kit (Thermo Fisher), or from COV434 cells and hiPSCs using the Monarch Total RNA Miniprep kit (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Immunology 2020Quote: ... Reactions with specific ISG/IFN or TATA-binding protein (TBP) housekeeper primers were prepared using the PowerUp SYBR Green Master Mix kit (ThermoFisher Scientific A25742) and loaded onto a 384-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... Bavi and 11.31 were used as PS-binding controls and stained with Alexa Fluor™ 594 Microscale Protein Labeling Kit (ThermoFisher, cat#A30008). Cells were washed twice with flow buffer prior to staining with BV421 Annexin V (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... NVX-CoV2373 Spike protein was coated on Maxisorp ELISA plate (Thermo Fisher), and then blocked with 5% BSA ...
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bioRxiv - Physiology 2022Quote: ... plasma C-reactive protein (CRP) was measured using an ELISA (Invitrogen, EM20RB). The ELISA was performed according to the manufactures protocol and the results were measured using a SpectraMax iD3 microplate reader (OD 450 nm) ...
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bioRxiv - Physiology 2023Quote: EGFR protein was quantified in cortical capillary preparations by ELISA (Invitrogen, EM23RB) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... pET SUMO (small ubiquitin-like modifier) expression vector (Invitrogen) bearing Mpro from SARS-CoV-2 gene with N-terminal His-SUMO tag was transformed into E ...
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bioRxiv - Cancer Biology 2022Quote: ... or Human Plasma-Like Media (HPLM, Gibco Cat. #A4899101) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... ‘stem-like’ conditions with B27 supplement (12587-010, Gibco), insulin ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.025% Insulin (GIBCO), 100x Glutamax supplement ...
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bioRxiv - Bioengineering 2022Quote: ... minus insulin (Gibco), 4 μM CHIR99021 (Selleck) ...
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bioRxiv - Neuroscience 2019Quote: ... insulin (1X, GIBCO) and forskolin (5 ng/mL).
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bioRxiv - Developmental Biology 2022Quote: ... 0.025% Insulin (GIBCO), 100x Glutamax supplement ...
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bioRxiv - Bioengineering 2020Quote: ... minus insulin (Gibco) containing CHIR99021 (Selleck ...
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bioRxiv - Molecular Biology 2023Quote: ... insulin (0.125%; Invitrogen), transferrin (0.125% ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... insulin (Gibco, A11382II) at 10 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... was conducted with intranuclear Transcription Factor Staining Buffer Kit (Invitrogen) in accordance with manufacturers recommendations ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed using FoxP3 Transcription Factor staining kit (Invitrogen) for 30 min and incubated with antibodies against intracellular proteins for 30 min ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: DNA regions corresponding to the coding sequence of each uL18-Like protein but lacking the stop codon were PCR amplified and transferred into pGWB5 (22) by Gateway™ cloning (Invitrogen) to create GFP translational fusions ...
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bioRxiv - Neuroscience 2020Quote: ... rat astrocytes were plated at 6-8K per well in pre-warmed DMEM (Invitrogen), supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: High affinity protein-binding 96-well plates (Nunc™ Maxisorp™ Cat No. 442404) were coated with 1 μg of protein (100 μL of 10 μg/mL of protein solution in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The expression levels were normalized against TATA binding protein (TBP) Mus musculus (Mm01277042_m1,ThermoFisher) and Ubiqutin C (UBC ...
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bioRxiv - Immunology 2022Quote: ... flat-bottomed Nunc MaxiSorp high protein-binding capacity 96-well plates (Thermo Fisher Scientific) were coated overnight at 4°C with the recombinant antigens (0.1µg/well ...
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bioRxiv - Microbiology 2022Quote: ... 1.2 ml of bacterial cultures were centrifuged in Low Protein Binding tubes (ThermoFisher Scientific) at 800 × g for 2 min at RT and the supernatant was removed ...