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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: Samples were suspended in 0.1% TFA and an equivalent to 1 µg of peptides was analyzed using Ultimate 3000 liquid chromatography system coupled to an Orbitrap QE HF (Thermo Fisher). An in-house packed analytical column (75 µm x 200 mm ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Systems Biology 2021Quote: ... lyophilized peptides were resuspended in 105 µl of equilibration buffer (1% TFA/ 80% ACN) and the peptide concentration was measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using equilibration buffer ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Immunology 2021Quote: ... RNA-optimized Spike genes were designed based on the SARS-CoV-2 Spike protein sequence from Wuhan-Hu-1 (GenBank: MN_908947) with its original signal peptide and the DNAs were synthesized by GeneArt (Thermo Fisher Scientific). All Spike plasmids have the 2P stabilizing mutations by substituting of K986V987 [34–36] ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested peptides were diluted to 60 μL with water and labeled for 1 hour at room temperature using 16plex TMTpro (Thermo Scientific) or 10plex TMT (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/10 of peptide solution was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (Thermo Scientific,Waltham MA, USA). Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of re-suspended peptide was injected per replicate using and Easy-nLC 1200 system (LC140; Thermo Scientific) mounted with an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Biochemistry 2023Quote: ... at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips, 100-μl bed; Thermo Fisher Scientific). Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg ...
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bioRxiv - Zoology 2021Quote: ... We standardized C-peptide measurements by dividing C-peptide concentrations by creatinine concentrations (measured via enzyme immunoassays with an auto analyzer: Gallery, Thermo Fisher Scientific, Waltham, MA, USA). C-peptide levels are therefore reported as pg/mg of creatinine ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 50 mM TEAB and their concentrations were determined using the Pierce™ quantitative fluorometric peptide assay (Thermo Fisher Scientific, Waltham, MA). Twelve micrograms of peptides were labelled with TMT labels (6-plex ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten labeled samples in each of TMT 10-plex sets were pooled together and 80 µg of labeled tryptic peptides were then fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo-Fisher Scientific, San Jose, CA) with a 9-step isocratic elution ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting peptides were separated during a 1 h gradient on C18 material using an Ultimate3000 rapid separation liquid chromatography system (Thermo Fisher Scientific) and subsequently injected in an online-coupled QExactive plus mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μg peptides were resuspended in 1% formic acid diluted in water and loaded on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). Settings used were as previously described [22] ...
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bioRxiv - Immunology 2020Quote: ... 1 µg of digested peptides were loaded into mass spectrometry system (Q Exactive Hybrid Quadrupole-Orbitrap MS manufactured by ThermoFisher Scientific, USA). For separation of peptides Hypersil gold C18 (100x2.1 mm ...
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bioRxiv - Molecular Biology 2023Quote: Tryptic peptides were separated on Hypersil Gold AQ C18 RP column 100 mm x 1 mm 3 µm 175 Å (Thermo Scientific) using UltiMate 3000 LC system (Dionex) ...
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bioRxiv - Neuroscience 2020Quote: ... 50 μL CSF was mixed with 50 μL of an internal standard mixture containing stable isotope-labelled peptides (JPT Peptide Technologies GmbH, Berlin, Germany; Thermo Fisher Scientific Inc. Waltham, MA, USA), 13C-labelled ubiquitin (Silantes ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were removed with a C18 cartridge (Thermo Scientific HyperSep, #03251257). Column cartridges were conditioned by successive treatment with methanol ...
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bioRxiv - Cancer Biology 2021Quote: ... Resultant peptides were labelled with TMT 6-plex reagents (Thermo Scientific) at an 8:1 tag:protein ratio as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted peptides were ionized by an EASY-Spray source (Thermo Scientific) and introduced directly into the mass spectrometer.
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bioRxiv - Microbiology 2021Quote: ... desalted on Pierce peptide desalting spin columns (Thermo Fisher Scientific # 89851), and dried in a vacuum concentrator.
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bioRxiv - Microbiology 2021Quote: ... desalted on Pierce peptide desalting spin columns (Thermo Fisher Scientific # 89851), and dried in a vacuum concentrator.
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bioRxiv - Systems Biology 2020Quote: ... Peptide concentration was determined by UV spectrophotometry (Nanodrop Spectrophotometer 2000, Thermofisher) at 280 nm ...
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bioRxiv - Biophysics 2022Quote: ... Separated peptides were detected by LTQ Orbitrap XL (Thermo Fisher Scientific) in positive-ion mode for m/z 200 – 2000 using electrospray ionization ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated on an EASY-nLC 1200 system (Thermo Scientific) at a flow rate of 300 nl/min over an 80 min gradient (5 % acetonitrile in 0.1 % formic acid for 4 min ...
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bioRxiv - Immunology 2020Quote: ... Peptides were introduced to the Fusion Lumos mass spectrometer (Thermo Scientific) using an Easy-Spray source at 2000V and 40°C ...
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bioRxiv - Neuroscience 2021Quote: ... the peptide was resuspended in anhydrous dimethylsulfoxide (DMSO) (Invitrogen, MA, USA) to a concentration of 5 mM ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were eluted by incubation with HA peptide (ThermoFisher) for 2 × 15 minutes at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Tryptic peptides were separated using nano-HPLC (UltiMate 3000, Thermo Scientific) coupled online with a Q-Exactive Plus hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Eluted peptides were dried in a Speedvac concentrator (Thermo Scientific, USA). Peptides (8 μl ...
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bioRxiv - Cancer Biology 2020Quote: Peptide identification was performed using Proteome Discoverer (version 1.4; Thermo Scientific) using Sequest-HT as search engine for protein identification ...
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bioRxiv - Systems Biology 2020Quote: Peptides were analyzed on an EASY-nanoLC 1200 (Thermo Fisher Scientific) coupled online to a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were quantified using the Pierce MicroBCA Kit (Thermo Fisher Scientific) following the instructions of the manufacturer.
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bioRxiv - Biophysics 2021Quote: ... Peptides were then separated with a C8 analytical column (Thermo Scientific BioBasic-8 5 μm particle size 0.5 mm ID x 50 mm 72205-050565 ...
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bioRxiv - Biochemistry 2020Quote: ... The quantitative colorimetric peptide assay kit was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were cleaned with PepClean C18 spin columns (Thermo Fisher Scientific), dried in a speed-vac ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated peptides were analyzed with an LTQ Orbitrap Elite (Thermo Scientific) in a data-dependent manner ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide labeling with TMT 10plex (90110; Thermo Fisher Scientific, Waltham, MA) was performed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Peptides were resuspended in 0.1% FA in water (SC235291, Thermo Scientific) prior to mass spectrometry analysis.
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were desalted using C18 tips (Thermo Scientific, Waltham, MA) according to the manufacturer’s guidelines before speed-vac and subsequent LC-MS/MS analysis.
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bioRxiv - Cell Biology 2021Quote: ... Peptides were analyzed using a Q exactive mass spectrometer (Thermo Scientific) coupled to an EASY-nLC 1000 liquid chromatography system (Thermo Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... The peptides were desalted with Pierce™ C18 Tips (Thermo Scientific), 100μl bed according to manufacturer instructions and peptides were eluted in 100μl of 75% ACN in 0.1% FA ...
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bioRxiv - Systems Biology 2020Quote: ... Peptide concentrations were measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using buffer A* ...
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bioRxiv - Systems Biology 2020Quote: ... Peptide concentrations were measured optically at 280nm (Nanodrop 2000, Thermo Scientific) and subsequently equalized using buffer A* ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated peptides were pulled down using streptavidin-conjugated magnetic beads (Invitrogen), and the precipitates were analyzed by immunoblot analysis
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bioRxiv - Cell Biology 2020Quote: Peptides were analysed on a Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a nano-electrospray ion source (Thermo Scientific ...