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Citations for Thermo Fisher :
501 - 550 of 5170 citations for Prothymosin alpha Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... or Pierce Peptide Desalting Spin Columns (Thermo Scientific) for monovalent or crosslinked samples ...
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bioRxiv - Biochemistry 2024Quote: ... the PierceTM Quantitative Colorimetric Peptide Assay (Invitrogen #C10416) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Acidified peptides quantified using Qubit 4 Fluorometer (ThermoFisher). All following spin steps occur at 1,600 x g unless otherwise noted ...
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bioRxiv - Biochemistry 2024Quote: ... and Pierce peptide desalting spin columns (89852, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The molecular mass of purified peptide was determined by 18% SDS-PAGE and the peptide concentration was obtained by Bicinchoninic protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Cell Biology 2019Quote: ... covered with medium for cryopreservation composed of MEM alpha (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 12.5 % hPL (PLSolution ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: Passage 5 EGFP-pMSCs [19] were cultured in minimum essential medium alpha (MEM-α) (Gibco) supplemented with 10% fetal bovine serum (HyClone ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RK13 cells were cultured in MEM-alpha supplemented with 10% fetal bovine serum (FBS; Gibco), 2 mM L-glutamine (HyClone ...
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bioRxiv - Cancer Biology 2021Quote: MM-1 feeder cells were cultured in MEM Alpha+GlutaMAX medium (ThermoFisher Scientific, Winsford, UK) supplemented with 10% FBS (Gibco) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Western blot for alpha-synuclein was performed using 4–12% Bis-Tris gels (Life Technologies), the protein was transferred onto 0.45 µm Millipore PVDF membrane (Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Smooth Muscle Actin Alpha (SMA or ACTA2, Invitrogen, PA5-18292, RRID: AB 10980764, 1:100), Anti-Goat 488 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... and transformed into DH5-alpha cells according to the manufacturers instructions (Subcloning efficiency, ThermoFisher 18265017). Clones were sequenced (GATC-Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting wild-type KIF18A inducible cell line was maintained in MEM Alpha (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Larval zebrafish (6 to 7dpf) were paralyzed with alpha-bungarotoxin 2mg/ml (Invitrogen, Waltham, USA), then embedded dorsal side up in 50μl of 1.2% low-melting point agarose (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used were as follows: mouse anti alpha-SMA (1:200, Invitrogen, Carlsbad, CA, USA); Myosin-IIB (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... BT-20 cells were maintained in Minimum Essential Medium Alpha (MEMα; Thermo Fisher, #41061-029) supplemented with 10% FBS and 1% Pen/Strep.
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bioRxiv - Cell Biology 2020Quote: ... Anti-alpha-tubulin was detected using Alexa Fluor 488-goat anti mouse (Invitrogen, 1:1000). In addition ...
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bioRxiv - Systems Biology 2019Quote: Alpha-Smooth Muscle Actin (Mouse monoclonal antibody, IgG2a, Clone: 1A4; Pierce/Invitrogen, cat#MA5-11547) was diluted to 1:200 with Ki-67 (D3B5) ...
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bioRxiv - Biochemistry 2021Quote: ... The collected cells were cultured in complete alpha minimum essential medium (α-MEM; Gibco, USA) containing 1% penicillin-streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: Primary antibodies were purchased as follows: rat anti-alpha tubulin (MA1-80017, Thermo Fisher Scientific), mouse anti-alpha tubulin (62204 ...
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bioRxiv - Bioengineering 2020Quote: ... and kept in growth media consisting of minimum essential alpha medium (αMEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: BMDMs were generated by culturing mouse bone marrow cells in alpha-MEM (Fisher Scientific, MT15012CV) containing 10% (vol/vol ...
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bioRxiv - Cancer Biology 2022Quote: ... and mouse anti-alpha smooth muscle actin (Thermo Fisher Scientific; 14-9760-82; 1:400). The secondary antibodies were horseradish peroxidase- conjugated goat anti-rabbit IgG antibodies (Nichirei ...
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bioRxiv - Bioengineering 2022Quote: ... Isolated rat bone marrow MSCs were cultured in alpha MEM (ThermoFisher Scientific, Burlington ON, Canada) with 10% fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... washed 2x with PBS and changed to serum free alpha-MEM (Invitrogen, Waltham, MA, USA) with 1% pencilin-streptomycin ...
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bioRxiv - Physiology 2023Quote: Balb/c mice were injected subcutaneously (s.c.) with 40IU of EPO (Recombinant HuEpo-alpha, Gibco) for four consecutive days ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.03% (w/v) L-glutamine (Alpha Caesar #A14201) 2% (w/v) sucrose (Fisher Scientific #10634932), and 100 μM acetosyringone ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated with alpha-bungarotoxin conjugated with Alexa594 (α-btx594, 4h; Thermo Fisher Scientific) to stain the acetylcholine receptors (AChRs) ...
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bioRxiv - Cancer Biology 2022Quote: ... with the addition of Super Bright 600 anti-CD49f (integrin alpha 6) antibody (Thermo Scientific, 63-0495-42 ...
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bioRxiv - Immunology 2023Quote: ... and TNF (PE rat anti-mouse TNF alpha, 1:100; Invitrogen, Cat. 12-7321-82) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in Alpha Minimum Essential Medium (α-MEM; GIBCO BRL, Grand Island, NY, USA) supplemented with 15% (v/v ...