Labshake search
Citations for Thermo Fisher :
51 - 86 of 86 citations for Prostaglandin H2 Analog since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Then 5mg of an analog of 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (FAST DiI, Invitrogen, D7756) in 250µL of 100% methanol (MeOH ...
-
bioRxiv - Bioengineering 2020Quote: ... encapsulated spheroids were incubated with the methionine analog azidohomoalanine (Click-iT AHA; Invitrogen, C10102; 100 μM) in methionine-free DMEM (custom made ...
-
bioRxiv - Biophysics 2020Quote: The spin-labeled cholesterol analog (androstane spin label [ASL]) was purchased from Molecular Probes (Eugene, OR); see the structures in Fig ...
-
bioRxiv - Immunology 2020Quote: ... For assessment of PGE2 production from monocyte cultures with a Prostaglandin E2Direct Biotrak Assay used according to the manufacturers protocol (Fisher scientific).
-
bioRxiv - Cell Biology 2022Quote: ... Constructs of hCAP-H2 harboring charge-reversal mutations in the BC1 and BC2 regions (H2-BC1D, BC2D, and BC1/2D) were synthesized by ThermoFisher and subcloned into in pFastBac1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The MSCs/MPCs or AML cells were harvested and washed with PBS and incubated with 2 µM of H2-DCFDA (C6827, ThermoFisher) in DMEM at 37°C for 40 minutes to detect ROS level ...
-
bioRxiv - Cell Biology 2022Quote: ... were crossed with knockout strain in BY4741 background (MATa, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;gene::KANMX) (Yeast KO Library, Mat A complete set, Thermofisher Scientific cat no. 95401.H2) as described previously [50] ...
-
bioRxiv - Microbiology 2024Quote: H2 concentrations in the culture headspaces were determined with gas chromatography with a thermal conductivity detector (Trace 1310, Thermo Scientific, USA). For sulfate analysis ...
-
bioRxiv - Immunology 2024Quote: ... cells were labelled with 150 μM of the fluorescently labeled glucose analog 2-NBDG (Thermo Fisher Scientific, N13195) or with 1µM bodipy (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... myotubes were transfected with 1µg of the MHC I (H2-kb) overexpression plasmid using Lipofectamine™ 2000 reagent for a period of 6 hours (Invitrogen, USA), both without and with the addition of the ER stress blocking agent ...
-
bioRxiv - Cell Biology 2022Quote: ... The H2-derived scFv was designed based on the amino acid sequences of the variable regions and synthesized by GeneArt (Thermo Fisher Scientific). The plasmid encoding H2 IgG is available from Addgene ...
-
bioRxiv - Cell Biology 2020Quote: ... Glucose uptake assay was measured as previously described using the fluorescent D-glucose analog 2NBDG (200 μM; Molecular Probes)35 ...
-
bioRxiv - Cell Biology 2020Quote: ... Glucose uptake assay was measured as previously described using the fluorescent D-glucose analog 2NBDG (200 μM; Molecular Probes) [15] ...
-
bioRxiv - Cell Biology 2022Quote: ... The medium was exchanged with fresh medium containing Hoechst stain (4µM) and 2-NBDG fluorescent glucose analog (100µg/ml) (ThermoFisher Scientific) and cells were incubated for additional 30 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... an amino acid analog of methionine containing an alkyne moiety that can be biotinylated by click-it chemistry (ThermoFisher), for nascent protein translation labeling followed by SDS-PAGE and HRP-streptavidin Western analysis ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed with PBS and incubated with 100 μL of 5 M H2-DCFDA in PBS (Molecular Probes, catalogue number D399). H2O2 levels were assayed on a fusion spectrofluorimeter (PackardBell ...
-
bioRxiv - Microbiology 2023Quote: The collected 2H-labelled water was analyzed using platinum catalyzed equilibration of 2H in H 2O with H2 gas by a Gasbench II headspace sampler connected to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher, Bremen, Germany). The sampled 18O-labelled water was analyzed through equilibration of 18O in H 2O with CO2 by a Gasbench II headspace sampler connected to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... tail and genomic RNA was transcribed from the SP6 promoter in the presence of N7mG cap analog using the SP6 mMessage mMachine kit (Ambion). 1×107 BHK21 cells were electroporated with approximately 2 μg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibiotics were used for double-drug resistant hybrid selection: Geneticin (Neomycin analog; Thermo Fisher; Neo 50 µg/mL), Hygromycin B (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... St3GFP or St3 Halo for 24 hours and treated with 1 μM nicotine for 17 hours. Neurons were labeled with 25 μM Nifrorhodamine (analog of Nifrofam, (Samra, Intskirveli et al. 2018)) or pHRodo (Thermofisher) as per manufacturers instruction for 30 minutes ...
-
bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells at exponential growth phase were incubated with 200 μM of the thymidine analog EdU (5-ethynyl-2′-deoxyuridine - Invitrogen) at 26 °C for 10.2 h (estimated doubling time of L ...
-
bioRxiv - Microbiology 2024Quote: ... tail and genomic RNA was transcribed from the SP6 promoter in the presence of N7mG cap analog using the SP6 mMessage mMachine kit (Ambion). 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad ...
-
bioRxiv - Immunology 2022Quote: ... with a tyramide-barcode staining cycle performed for mouse anti-CD30 primary antibody (1:50 dilution, clone Ber-H2, Invitrogen, catalog number MA5-13219), and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution ...
-
bioRxiv - Cell Biology 2020Quote: DNA synthesis was assessed by thymidine-analog incorporation and histological detection using the Click-iT EdU Imaging Kit (Thermo Fisher C10337). Confluent serum-deprived Calu-3 cells on coverslips were scratched ...
-
bioRxiv - Cancer Biology 2020Quote: Cells (105/well in 12-well plates) were incubated with 5µM of the fluorescent glucose analog 2NBDG (N13195, Thermo Fisher Scientific) in Opti-MEM™ I Reduced Serum Medium (31985088 ...
-
bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
-
bioRxiv - Microbiology 2023Quote: ... difficile strains were cultured anaerobically (10% H2, 10% CO2, and 80% N2) at 37℃ on BHI agar or in BHI broth (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 0.05% L-cysteine and yeast extract and tryptone–yeast extract (TY ...
-
bioRxiv - Bioengineering 2021Quote: ... The complexes were prepared by mixing these solutions and vortexed at a setting of ‘5’ on a vortex mixer (Fisher Analog Vortex, 120V, Fisher Scientific, USA) for 30 secs ...
-
bioRxiv - Cell Biology 2021Quote: ... the fatty acid analog C1-BODIPY 500/510 C12 was incubated for 6h at a final concentration of 2μM (Thermo Fisher Scientific, #D3823). Images were acquired on a Leica TCS SP8 equipped with a 405-nm laser line and a white light laser with a 63x/1.4 DIC Lambda blue Plan Apochrome objective ...
-
bioRxiv - Bioengineering 2021Quote: ... The HBSS was removed and cells were then incubated at 37°C in HBSS containing the methionine analog azidohomoalanine (Click-iT AHA; Invitrogen, C10102; 100 μM) for 1 hour ...
-
bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction for the 5’ cap driven Fluc reporter included a 1:4 ratio of Invitrogen™ Anti-Reverse Cap Analog (Fisher Scientific AM8045) and GTP solution (0.4 µl cap analog to 1.6 µl of GTP solution) ...
-
bioRxiv - Neuroscience 2020Quote: Activity monitors and recording arenas containing flies were placed on a shelf ∼40 cm above an analog multi-tube vortexer (Fisher Scientific, Pittsburgh, PA) or secured on the speaker system platform using screws ...
-
bioRxiv - Plant Biology 2020Quote: ... in water solution was then added to each vial and vortexed on a plate shaker (Fisherbrand™ Analog Multitube Vortexer, ThermoFisher Scientific, Waltham, MA, USA) at 4 °C at max speed (2500 rpm ...
-
bioRxiv - Neuroscience 2023Quote: ... were visualized using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43FX, a fixable analog of FM1-43 membrane stain, Thermo Fisher Scientific, Waltham, MA, USA). To stain the presynaptically active synapses of autaptic cultured neurons ...