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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... as a Cas9-RNP complex (1:1 Cas9:gRNA molar ratio) were delivered primary human CD3+ T cells after 2 days of Anti-CD3/-CD28 Dynabead (ThermoFisher) stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... Viably frozen primary AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24h before transplant ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Cell Biology 2023Quote: ... E6 medium was changed daily up to 4 days before switching to human endothelial serum free medium (hESFM) (Thermo Fisher Scientific, 11111) supplemented with 10nM bFGF (Fibroblast growth factor ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Immunology 2019Quote: ... suspended in endothelial cell growth media containing with SYTOX orange (1 μM, Thermo Fisher Scientific) to visualize DNA and flowed through the HUVEC-lined channels at 5 dynes/cm2 for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Endothelial cells were stained with CellTracker™ Green CMFDA Dye (1 μM, Molecular Probes, #C7025) according to manufactures protocol ...
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bioRxiv - Physiology 2023Quote: ... Endothelial cells were sonicated 10 times on setting 3 (Sonic Dismembrator Model 100, Fisher Scientific) in lysis buffer (20 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Brain endothelial cell monolayers were plated on Lab-Tek® chamber slides (Thermofisher Scientific; #1777399PK) and exposed to cytokines (IL-1β ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was extracted from endothelial cells and astrocytes by adding 300 µl of Trizol (ThermoFisher) to the cell pellet ...
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bioRxiv - Neuroscience 2024Quote: Mouse-hosted antibodies [anti-rat endothelial cell antigen (RECA-1; 1:50, MA1-81510, Invitrogen), anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Tumor-supplying endothelial cells were isolated from single cell suspensions by magnetic bead sorting of CD31+ cells using Dynabeads® CD31 Endothelial Cells (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Aortic protein extraction was carried out using a radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher Scientific Inc.), supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Physiology 2020Quote: ... M1 and M2 primary human skin fibroblasts were maintained in MEM (Fisher Scientific) supplemented with 20% fetal bovine serum and 2% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2019Quote: ... Primary MMP13 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-14247) was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human ...
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bioRxiv - Biochemistry 2020Quote: ... coated with primary antibody CD63 (Exosome-Human CD63, Thermo Fisher - Scientific - Invitrogen™) and CD81 (Exosome-Human CD81 ...
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bioRxiv - Biochemistry 2020Quote: ... coated with primary antibody CD63 (Exosome-Human CD63, Thermo Fisher - Scientific - Invitrogen™) and CD81 (Exosome-Human CD81 ...
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bioRxiv - Molecular Biology 2023Quote: The RNA was extracted from human primary OCs using the TRIzol Reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue culture flasks until they reached 70-80% confluence in human ectocervical primary cell medium (Advanced DMEM/F-12 (Invitrogen, 12634) supplemented with 12 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... astrocytes and endothelial cells (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, ThermoFisher; CD45.1 clone A20 in BV421 (BD); CD45.2 clone 104 in BV421 (BD) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were expanded with serum-free basal endothelial cell medium (EC medium) supplemented with 50x B27 (Thermo Fisher Scientific), GlutaMAX™ (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes and endothelial cells (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, ThermoFisher; CD45.1 clone A20 in BV421 (BD); CD45.2 clone 104 in BV421 (BD) ...
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bioRxiv - Microbiology 2019Quote: ... including human 293FT cells (Invitrogen), rat2 cells (ATCC #CRL-1764) ...
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bioRxiv - Cancer Biology 2019Quote: ... Human Epidermal Melanocyte cells (Invitrogen) were cultured in Medium 254 (Invirogen ...
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bioRxiv - Immunology 2021Quote: Human iPS cells (Thermo Fisher) were cultured on vitronectin-coated T225cm2 flasks using complete mTesSR Plus medium (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Human Expi293 cells (Thermo Scientific) were transfected with these constructs using FectoPro (PolyPlus Transfection ...
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bioRxiv - Bioengineering 2020Quote: ... we imaged a commercial slide of bovine pulmonary artery endothelial cells (BPAEC) (Life Technologies, Carlsbad, CA). The mitochondria in the live BPAEC were stained by MitoTracker® Red CMXRos with accumulation dependent upon membrane potential ...
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bioRxiv - Cancer Biology 2020Quote: ... assumed CD31 + endothelial cells were re-suspended in DMEM media with 5% MVGS (Gibco, Cat# S00525) and 5% FBS and seeded in a gelatin-coated dish.
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bioRxiv - Physiology 2023Quote: ... Frozen endothelial cells were sonicated 10 times on setting 3 (Sonic Dismembrator Model 100, Fisher Scientific) in the proprietary lysis buffer provided with protease inhibitors (10 µM leupeptin ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells were gently recovered from tissue culture plates using TrypLE Express (Invitrogen, Grand Island, NY) to maintain surface antigen expression for 10-15 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... corneal endothelial cells were cultured in M4 proliferation medium (1:1 Ham’s F12 (Thermo Fisher Scientific) and M199 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial media (EC): hESFM (Thermo Fisher, Cat#: 11111044), 50X B27 supplement (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... with Large Vessel Endothelial Supplement (LVES, ThermoFisher Scientific) and penicillin (10 U/ml)/streptomycin (10 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Molecular Biology 2019Quote: ... The mouse aortic root specimens were cut into 8 μm sections using a freezing microtome (Thermo Scientific). Some sections of aortic root were stained by oil-red O to investigate atherosclerotic lesion ...
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bioRxiv - Cell Biology 2023Quote: ... Aortic root cryosections were dried at RT for 10 minutes and fixed in acetone (Acros organics, 423245000) at -20⁰C for 3 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human lung fibroblasts were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific ...