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Citations for Thermo Fisher :
651 - 700 of 1806 citations for PVRIG Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...
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bioRxiv - Cell Biology 2021Quote: ... A 1:10,000 dilution of mouse α-(6X)His (Invitrogen) primary antibody in PBST/3% BSA was applied for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in 12μl of Hi-Di formamide (Thermo fisher, 4311320), denatured at 95°C for 5 mins followed by snapchill ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) or Herculase II Fusion (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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bioRxiv - Immunology 2020Quote: ... 1 mg of magnetic bead (Dynabeads™ His-Tag, Invitrogen) were coated with 70 μg of histidine tagged RBD ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... secondary anti-6x-His tag monoclonal antibody (Invitrogen, # MA1-21315) at 1:175 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Neuroscience 2023Quote: ... 6x-His Tag Monoclonal Antibody (HIS.H8) (MA1-21315, Thermo Scientific) and HRP-conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned it in the pcDNA3.1/myc-His(-) plasmid (Invitrogen) in frame with a Myc-His tag to obtain the wild-type (WT ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween overnight ...
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...
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bioRxiv - Biophysics 2024Quote: 6x-His epitope tag antibody (His.H8) was purchased from Invitrogen. Monoclonal Flag M2 antibody (F1804-50UG) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The fibroblasts and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (#11965–092; Gibco, NY) supplemented with 10% FBS (#12483-020 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573, RRID:CVCL_0045) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and HEK293 cells were cultured in DMEM with sodium pyruvate and GlutaMAX (Life Technologies 10569044) or 2 mM L-glutamine supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were subjected to drug selection in the medium with 2 μg/ml puromicin (Invitrogen) for 14 days ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: Flp-In™ T-Rex™ HEK293 cells (Life Technologies, we did not test for mycoplasm) were cultured following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... expressing mouse ATG16L1 variants was transfected into HEK293 ATG13_KO GFP-LC3B cells via Lipofectamine 2000 (Invitrogen). Cells were selected with 2.5 μg/ml puromycin (P8833 ...
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bioRxiv - Biophysics 2020Quote: ... HEK293 cells were transfected (in 35-mm-diameter wells) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and plated onto coverslips coated with Matrigel (BD Biosciences ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2022Quote: BiP-3xFLAG HEK293 cells (0.12 × 106) were plated on 12 mm round glass coverslips (Fisher Scientific) coated with 0.15 mg/mL poly-lysine in 24-well plates ...
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bioRxiv - Plant Biology 2022Quote: HEK293 cells were transfected with the respective eYFP-TPC1-fused constructs with lipofectamine2000 (ThermoFisher, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... siRNA duplexes (siRNA B and C) were transfected into HEK293-PSMB2-HB cells using RNAiMAX (Invitrogen) dissolved in Opti-MEM (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 (ATCC) were transfected using Lipofectamine LTX® Reagent and OptiMEM Reduced Serum Medium (Thermo Fisher) with pCMV-MAPK14 Wt (DU1765 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with relevant plasmids or siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific), and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... B(-)S13−) bacmids were transfected into HEK293 cells using Neon transfection system (Invitrogen, Carlsbad, CA, USA). 5 x 104 cells were resuspended in 100 μl of serum-free media containing 5ug of each mutant recombinant bacmid ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were rinsed with cold PBS then scraped in PBS+1xHALT protease/phosphatase inhibitor (ThermoFisher). The cell slurry was centrifuged for 5 min at 1,000xg at 4°C to pellet cells ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Genetics 2022Quote: ... HEK293 cell cultures were transfected with 15 μg of the plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: HEK293 and A549 cells were transfected with 15 pmol of siRNA using Lipofectamine RNAiMAX (Life Technologies). siRNAs (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells cultured in Dulbecco’s Modified Eagle medium plus GlutaMAX (DMEM, Thermo Fisher Scientific, MA, USA) supplemented with 10 % (v/v ...
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bioRxiv - Physiology 2020Quote: ... All constructs were transfected into HEK293 cells using a TurboFect transfection reagent (ThermoFisher Scientific, Waltham, MA). The day before transfection ...
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bioRxiv - Neuroscience 2021Quote: All the cells (MEF, N2a and HEK293) were plated onto tissue culture dishes in DMEM (Invitrogen) supplemented with 10% FBS and 1% Pen/Strep (Gibco ...