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Citations for Thermo Fisher :
701 - 750 of 7360 citations for PDIA5 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The medium was replaced with FreeStyle 293 medium (ThermoFisher) supplemented with 5 µg/mL brefeldin A (Enzo Life Science BML-G405 ...
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bioRxiv - Microbiology 2022Quote: ... the medium was refreshed using FreeStyle 293 medium (ThermoFisher). Cell lysates and supernatants were harvested at 48 h post-transfection and processed as described above.
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bioRxiv - Molecular Biology 2023Quote: ... The Plasmid was transiently transfected using Expifectamine 293 (GIBCO) and incubated at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... suspension cells were cultured in 293 Expression Medium (Gibco) supplemented with 1% FBS at 37□°C in CO2 incubator ...
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bioRxiv - Biophysics 2023Quote: Flp-In™/T-Rex 293 cells from Invitrogen stably expressing TetOn inducible SNAP-β1AR were established by co-transfection with an excess of pOG44 plasmid encoding Flp recombinase and pcDNA™ 5/FRT/TO/SNAP-β1AR expression vectors.
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bioRxiv - Biochemistry 2023Quote: The T-RexTM-293 cell line (HEK293TR; ThermoFisher Scientific) was used to make stable expressing cell lines for receptors cloned into pcDNA4/TO ...
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bioRxiv - Molecular Biology 2023Quote: ... using ExpiFectamine 293 transfection as described (Thermo Fisher Scientific). 6-days post-transfection ...
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bioRxiv - Bioengineering 2022Quote: ... was grown in Freestyle 293 expression medium (ThermoFisher Scientific). CHOExpress® cells were cultivated according to supplier’s recommendation (Excellgene SA).
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bioRxiv - Biophysics 2023Quote: ... the fresh medium (FreeStyle 293 expression medium (Thermo Scientific) was added to dilute the cells to a density of 1 × 106 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... were maintained in 293 Freestyle medium (Thermo Fisher, 12338026) and were transiently co-transfected with IgH and IgL expression vectors for seven days prior to monoclonal antibody purification from the culture supernatant using Protein G Sepharose 4 Fast Flow beads (Cytiva,17-0618-05 ...
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bioRxiv - Neuroscience 2023Quote: Nanobody expression was performed with Expi 293 cells (ThermoFisher). Detailed expression and purification protocols can be found in (Han et al. ...
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bioRxiv - Biochemistry 2023Quote: FlpIn T-REx 293 cells (Thermo Fisher Scientific, R78007), referred to as HEK293T ...
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bioRxiv - Microbiology 2024Quote: ... with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Immunology 2023Quote: ... using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific, A14524). Cells were incubated for 4-5 days for protein expression ...
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bioRxiv - Microbiology 2023Quote: ... cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) were transfected with DENV VLP-expressing plasmids ...
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bioRxiv - Immunology 2023Quote: ... cells using the ExpiFectamine 293 transfection kit (ThermoFisher: A14525) and following previously described methods constructs[30] ...
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bioRxiv - Immunology 2023Quote: ... cells using the ExpiFectamine 293 Transfection kit (Gibco: A14525) and following previously described methods (11) ...
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bioRxiv - Immunology 2023Quote: ... with transfection reagents (ExpiFectamine™ 293 Transfection Kit, ThermoFisher). After 4 to 5 days ...
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bioRxiv - Biochemistry 2024Quote: ... grown in suspension in 293 Freestyle medium (Thermo Fisher). Cells were grown up to 106 cells/ml at 37°C CO2 8% in agitation at 125 rpm ...
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bioRxiv - Bioengineering 2024Quote: ... were grown in FreeStyle 293 expression medium (ThermoFisher 12338018) in 37°C ...
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bioRxiv - Biophysics 2024Quote: ... Parental Flp-InTM T-RExTM 293 cells (Invitrogen, R78007) were kept in high glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... cells from Gibco (R79007) were cultured in FreeStyle 293 expression media (Gibco) on a shaker with a speed of 35 rpm in a 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Cell Biology 2022Quote: ... doxycycline-inducible GFP-EMC2 Flp-In 293 T-Rex cell line and its adaptation to suspension growth in FreeStyle 293 Expression Medium (Thermo Fisher Scientific, USA) was described before (Pleiner et al. ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... MSC and HEK cell-lines were maintained in high-glucose (4.5 g/l) DMEM GlutaMax (Thermo Fisher Scientific, Waltham, MA, USA; HEK) or RPMI medium 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and cultured in the presence of hygromycin to select DC-SIGN expressing HEK-293T cells (DC HEK) (Thermo Fisher Scientific). Similarly ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK 293T cells were transfected using Lipofectamine 2000 (ThermoFisher), as per manufacturer’s instructions and standard procedures ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher) in a T-25 flask.
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bioRxiv - Cancer Biology 2020Quote: HEK 293T cells were cultured in DMEM media (GIBCO) supplemented with 10% FBS (GIBCO) ...
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bioRxiv - Bioengineering 2020Quote: HEK 293T cells were cultured in DEME (Gibco, 11995115) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... HEK-293T cells were transfected using Lipofectamine 2000 (Invitrogen) for lentiviral expression vector pLL5.0 and third generation packaging constructs pMDLg/pRRE ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293FT cells (R70007) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293A cells (R705-07) were purchased from Invitrogen. Murine 10.1 fibroblasts have been described [61] ...
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bioRxiv - Bioengineering 2022Quote: HEK 293T cells were cultured in DMEM (11885, Gibco) supplemented with 10% FBS (Gibco 10082147 ...