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Citations for Thermo Fisher :
1 - 50 of 4886 citations for PDGF BB Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Bioengineering 2024Quote: ... PDGF-BB (PHG0045; 50 ng/ml; Gibco-ThermoFisher, Waltham, MA) or both (25 ...
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bioRxiv - Bioengineering 2024Quote: ... PDGF-BB (PHG0045; 50 ng/ml; Gibco-ThermoFisher, Waltham, MA) or both (25 ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were added to the bottom of the plate with 600 μL of normal growth media containing 50 ng/mL of PDGF-BB (ThermoFisher) to induce chemotactic migration 30 ...
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bioRxiv - Immunology 2021Quote: ... MIP-1b(CCL4)), growth factors (BDNF, G-CSF, GM-CSF, PDGF-BB, VEGF-A) and other factors (CD40L, Granzyme B) (Invitrogen, Carlsbad, CA). Differences in induction of proteins post stimulation were tested using unpaired t-tests with Welch’s correction ...
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bioRxiv - Immunology 2021Quote: ... MIP-1b(CCL4)), growth factors (BDNF, G-CSF, GM-CSF, PDGF-BB, VEGF-A) and other factors (CD40L, Granzyme B) (Invitrogen, Carlsbad, CA). Differences in induction of proteins post stimulation were tested using both unpaired (Control-EtOH ...
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bioRxiv - Genetics 2023Quote: ... and 10 ng/ml PDGF (Life Technologies PHG1034). PhNPCs lines were split once per week using 0.05% Trypsin-EDTA (Life Technologies 25300062 ...
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bioRxiv - Genetics 2022Quote: ... and 20 ng ml−1 PDGF (Life Technologies, PHG1034). We followed previously established protocols to maintain NPCs as proliferating neural progenitors and inhibit differentiation into neurons31 ...
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bioRxiv - Genetics 2023Quote: ... and 10 ng ml−1 PDGF (Life Technologies PHG1034).
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bioRxiv - Cell Biology 2020Quote: ... PDGF-AA ELISA kits (EHPDGF) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 ng/mL Platelet-derived growth factor (PDGF, Invitrogen, USA) for 3 days at 37 °C under a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL each of platelet-derived growth factor (PDGF) (Gibco), and fibroblast growth factor (FGF ...
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bioRxiv - Cell Biology 2023Quote: ... platelet-derived growth factor-AB (PDGF-AB) (10 ng/ml, Thermo Fisher), tumor necrosis factor-α (TNF-α ...
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bioRxiv - Genomics 2023Quote: ... in 1x BBS (Thermo Fisher Scientific 28384) pH 8.5 ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was isolated from WT BBS and BBS mutant MCF7 cells using TRIZol (Ambion, 15596018, lot no. 260712) reagent and PureLink RNA Mini Kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 40 nM or rPDGF-BB (ThermoFisher Scientific PMG0044), or hormone vehicles (0.5% of water ...
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bioRxiv - Cell Biology 2019Quote: ... growth factor-depleted medium were stimulated with 10 ng/mL PDGF (Thermo Fisher Scientific, PHG0044) or 50 ng/mL of VEGF165 (R&D ...
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bioRxiv - Microbiology 2020Quote: The plasmid pMR-RQ-Bb/SA11g72AFLUnaG was obtained from ThermoFisher and contained a modified synthetic cDNA of SA11 segment 7 (NSP3) ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa488-conjugated goat anti-mouse (1:500 in BB, Invitrogen, A21133) or Alexa488-conjugated goat-anti-chicken (1:500 in BB ...
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bioRxiv - Developmental Biology 2020Quote: ... or Alexa488-conjugated goat-anti-chicken (1:500 in BB; Invitrogen, A11039). Embryos were washed in PBST before imaging.
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bioRxiv - Developmental Biology 2022Quote: ... consisting of cSFDM with 10 ng/ml rhPDGF-BB (Life Technologies, #PHG0046), 10 ng/mL rmTGF-β (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... pBv2-U6-6X and pBv1-U6-BB plasmids using Neon Transfection System (Invitrogen) under condition of 1050 V ...
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 chicken fibroblasts transfected with RCAS-PDGF and DF-1 cells transfected with RCAS-Cre were maintained in DMEM with 10% fetal bovine serum (Gibco). Heterozygous NTV-a;Ink4a-Arf+/-;Ptenfl/fl;LSL-Luc were generated by crossing NTV-a (WT ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibody diluted in BB (1:500) consisted of goat anti-mouse Oregon Green (488; Thermo Fisher Scientific) and goat anti-rabbit TRITC (Jackson Immunoresearch) ...
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bioRxiv - Neuroscience 2024Quote: ... A subconfluent 6-well dish of POE SH-SY5Ys were transfected with 1ug of each PINK1_Seq1 and PINK1_Seq2 sgRNA encoding pSpCas9(BB)-2A-GFP plasmid using lipofectamine 3000 (Invitrogen). 24hr post-transfection cells were trypsinised and subjected to fluorescence-activated cell sorting and clonal selection of GFP-positive cells.
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bioRxiv - Immunology 2024Quote: ... KP cells were transiently transfected with pZac2.1-U6sgRNA-CMV-ZsGreen and (pSpCas9(BB)(PX458) using Lipofectamine 3000 (Invitrogen), following the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Biophysics 2023Quote: ... The Alexa Fluor 647 rabbit@goat secondary antibody (2 µg/mL in BB, #A-21446, Invitrogen, Thermo Scientific, Germany) was added to the cells and incubated for 1 h at room temperature with gentle shaking ...
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bioRxiv - Biophysics 2023Quote: ... The Alexa Fluor 647 rabbit@goat secondary antibody (2 µg/mL in BB, #A-21446, Invitrogen, Thermo Scientific, Germany) was added to the cells and incubated for 1 h at room temperature with gentle shaking ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Cell Biology 2020Quote: ... The obtained DNA duplexes were ligated to pSpCas9(BB)-2A-Puro(PX459) V2.0 digested by BpiI (FD1014; Thermo Fisher Scientific). Claudin-2 KO cells were transfected with the CRISPR/Cas9 vectors using Lipofectamine LTX and Plus Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Positive pSpCas9(BB)-gRNA clones were confirmed by Sanger sequencing and expanded using PureLink® HiPure Plasmid Midiprep Kit (Invitrogen).
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... containing the sequence coding for eGFP and from the defective helper (pDH-BB) plasmid using the Mmessage Mmachine SP6 solution (Ambion). Both cRNAs were then electroporated into BHK21 cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Microbiology 2020Quote: ... 3.6 µg pSp-Cas9(BB)-2A-GFP-sgRNA and 400 ng pSc-TIA- CMV-BSR-TIA were mixed with polyethylenimine and OptiMEM (#51985026, ThermoFisher Scientific) for transfection of cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of 2 µg pSpCas9-BB-2A-GFP-sgYBX1 was carried out with 6 µl of Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed 3 x 15 min in WB at RT and secondaries were then diluted in BB blocking buffer (Donkey anti rabbit AF568 (Invitrogen #A10042) 1:750 ...
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bioRxiv - Genetics 2020Quote: The inducible XIST constructs within the HT1080 2-3-0.5a cells were modified by transiently transfecting two unique gRNA plasmids (pSPgRNA) and a Cas9 plasmid providing transient puromycin resistance (pSpCas9(BB)-2A-Puro) using Lipofectamine 3000 (ThermoFisher #L3000008). The day before the transfection the HT1080 cells were split into 24 well plates ...
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bioRxiv - Genomics 2021Quote: ... We co-transfected gRNA_cloning vectors (1 µg each) and pSpCas9(BB)-2A-Puro plasmid (1 µg) using lipofectamine 3000 reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were transfected with 5ug of pSpCas9(BB)-2A-GFP plasmid containing the respective sgRNA using Lipofectamine 3000 (Life Technologies, L3000008). After 24h incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...