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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for PAK4 5 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Genetics 2024Quote: ... Fixed cells were then washed in the blocking solution (0.3% BSA (Fisher Bioreagents, Cat# BP1600-100) and 0.01% Tween (Thermo Scientific, Cat# 9005-64-5) in 1x PBS) ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 25 fmol/µl standard peptide mixture (Pierce™ Peptide Retention Time Calibration Mixture, ThermoFisher, 88321) and diluted to a final volume of 23 µl with water ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
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bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were delivered onto a nano RP column (5-μm Hypersil C18, 75 mm × 100 mm; Thermo Fisher Scientific, Waltham, MA, USA) and eluted with escalating (50-80% ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: Oocytes were collected in porcine oocyte medium (Research Institute for the Functional Peptides, Yamagata, Japan) supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific Inc.) and 75 IU/mL FSH (Fuji Pharmaceuticals ...
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bioRxiv - Evolutionary Biology 2022Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... The peptides were separated on a nanoElute nanoHPLC system (Bruker, Bremen, Germany) using a 5 mm trapping column (Thermofisher Scientific, Waltham, MA, USA) and an in-house packed C18 analytical column (15 cm × 75 µm ID ...
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bioRxiv - Cell Biology 2023Quote: ... Tryptic peptides were loaded onto a trap column (Acclaim™ PepMap™ 100 C18, 3μm, 5 × 0.3 mm, Fisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 2% acetonitrile in 0.1% TFA as loading buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm, C18, 5 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 µL/min for 3 min ...
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bioRxiv - Neuroscience 2024Quote: ... Attached to the UPLC was a peptide trap (100 μm × 200 mm, 100 Å pore size, 5 μm particle size, C18, Thermo Fisher Scientific) for online desalting and purification followed by a 25 cm C18 reversed-phase column (75 μm × 250 mm ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with 5 mL of PBS and incubated with a blocking buffer comprised of 1% bovine serum albumin (BSA, Fisher Scientific cat. no. BP9703) and 10% goat serum (Sigma Aldrich cat ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...
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bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
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bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-peptide enrichment was then performed using the High-SelectTM TiO2 Phospho-peptide Enrichment kit (Thermo Fisher Scientific). Each replicate was divided between two TiO2 columns and prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: Phospho-peptide enrichment was carried out using the High-Select TiO2 Phospho-peptide Enrichment kit (Thermo Fisher, A32993), as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The peptide concentration of this pool was determined using a fluorimetric peptide assay kit (Thermo Scientific, Cat#23290). Peptide concentrations per sample were estimated by multiplying the optical density recorded at harvest with the ratio between pool peptide concentration and the median at-harvest optical density.
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bioRxiv - Cancer Biology 2023Quote: The peptide amount in each sample was estimated using a quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Within each batch ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptide mix was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific). The 8 obtained fractions were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... soluble peptide suspension was transferred into fresh Eppendorf tubes and peptide concentration was determined by microBCA (Thermo Scientific). Equal amounts of peptides for each sample (150-200 µg ...
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bioRxiv - Genetics 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce Quantitative Peptide Assays kit (ThermoFisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip ...
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bioRxiv - Microbiology 2020Quote: ... Blocking was carried out overnight at 4°C in a humidified dark chamber using Blocking Aid Solution (Thermo Fisher Scientific). Primary antibody diluted in Blocking Aid Solution (Anti-PvGAMA 1:1200 ...
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bioRxiv - Physiology 2019Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in phosphate-buffered saline (PBS) followed by an additional blocking step with 1% Tyramide Signal Amplification (TSA) blocking reagent (TSA kit, T20935, Invitrogen) and Mouse-on-Mouse blocking reagent (Vector Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... or HA peptide (Thermo Fisher Scientific, 26184).
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bioRxiv - Microbiology 2019Quote: ... Peptides were labelled with TMT6plex (ThermoFisher Scientific), desalted with solid-phase extraction on a Waters OASIS HLB µElution Plate (30 µm) ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted peptides were analyzed by Thermo Scientific Q Exactive or Q Exactive plus mass spectrometer utilizing a top 15 methodology in which the 15 most intense peptide precursor ions were subjected to fragmentation ...
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bioRxiv - Microbiology 2021Quote: ... All peptides were obtained from Thermo Fisher Scientific (Planegg ...