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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... ethynyl-2′-deoxyuridine (EdU) incorporation using Click-iT EdU imaging kit (Thermo Fisher). Cells were seeded onto collagen coated PAA gels of varying stiffnesses for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were sorted in 96-well plates (Piko PCR Plates 24-well, Thermo Scientific, SPL0240 and Plate Frame for 24-well PikoPCR Plates, Thermo Scientific, SFR0241). Each well contained Triton-X100 (0.2% ...
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bioRxiv - Molecular Biology 2021Quote: ... Enzymatic activity is inhibited by adding 5 ml of DPBS 1x (Gibco, 14190-094) containing 10% FBS and 1mM EDTA (Sigma #E7889) ...
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bioRxiv - Microbiology 2021Quote: ... the β-galactosidase activity was measured using the Galacto-Star™ system (ThermoFisher Scientific). For infections with lentiviral and retroviral vectors ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured in a Labsystems Luminoskan TL Plus luminometer (Thermo Scientific, USA) using a Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2019Quote: ... HRP activity was assessed using the SuperSignal™ West Pico Chemiluminescent substrate (ThermoFisher Scientific) and visualized on X-ray film.
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bioRxiv - Genomics 2019Quote: ... after which fluorescence activity was assayed on an Attune NxT Flow Cytometer (Thermo Fisher) with an excitation wavelength of 561 nm ...
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bioRxiv - Molecular Biology 2021Quote: DNase activity was determined using the DNase Alert QC System (AM1970, Thermo Fisher Scientific). Substrate solution and 10X nuclease buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Chymotrypsin activity was determined relative to trypsin using the Pierce fluorescent protease assay (ThermoFisher), and trypsin concentration was quantified using an enzymatic assay with Nα-Benzoyl-L-arginine ethyl ester (BAEE ...
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bioRxiv - Genomics 2022Quote: ... Further RNase activity was stopped by addition of SUPERaseIn RNase Inhibitor (Thermo Fisher Scientific). Next ...
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bioRxiv - Immunology 2022Quote: ... After blocking of endogenous peroxidase activity using Ultravision hydrogen peroxide block (Thermo Fisher Scientific), endogenous biotin was blocked (Lab Vision Avidin biotin Blocking Solution ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... Enzymatic activity is inhibited by adding 5 ml of DPBS 1x (Gibco, 14190-094) containing 10% FBS and 1mM EDTA (Sigma #E7889) ...
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bioRxiv - Neuroscience 2023Quote: ... Trypsin activity was blocked by washing with pre-warmed Neurobasal medium (Gibco, 21103-049) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... the ASK3 activity was measured and analyzed by using CellInsight NXT (Thermo Fisher Scientific) with HCS Studio (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Metabolic cellular activity was determined using alamarBlue™ HS Cell Viability Reagent (ThermoFisher, #50101). For droplet experiment ...
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bioRxiv - Genomics 2023Quote: ... Loss of enzymatic activity was confirmed by 3’-(p-aminophenyl) Fluorescein (APF) assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: GCase activity was measured by using the fluorogenic substrate PFB-FDGlu (Invitrogen, Carlsbad, CA). WT and R1441G LRRK2 mutant MEF cells were treated with 300μM CBE for 24h ...
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bioRxiv - Cancer Biology 2023Quote: ... PTP activity was assayed using 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, Invitrogen, cat# D6567,) as a substrate in 3,3-Dimethylglutaric acid (DMG ...
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bioRxiv - Bioengineering 2023Quote: ... The endogenous peroxidase activity was inhibited by incubating in peroxidase suppressor (Thermo Fisher Scientific) for 30 min and rinsed with PBS for 3 times ...
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bioRxiv - Molecular Biology 2023Quote: ... Peroxidase activity was detected with SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific, USA). For mCherry and mCherry-fused proteins detection ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...
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bioRxiv - Biophysics 2021Quote: ... 2 μl sticky LCP was dispensed onto the sidewall of a UV-transparent 96-well plate (Cat. 8404, Thermo Scientific, USA). 200 μl pre-warmed buffer F (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA) and 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Cells seeded on 13 mm coverslips placed in 24 well plates were transfected twice (on day 1 and 2) with Oligofectamine or RNAi MAX (Thermo Fisher) complexed with 20pmol of each indicated siRNA and analyzed 3 to 4 days after the first transfection ...
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bioRxiv - Genetics 2019Quote: ... YPD cultures were grown to saturation (∼24 hours) in a 96-well plate and ∼2 µl per well was transferred to OmniTrays (Nunc 264728) using a 96-pin multi-blot replicator (V&P Scientific no ...
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bioRxiv - Immunology 2019Quote: ... 50 μL of HLA binding neutralisation buffer was added to each well and the resulting supernatants were incubated for 2 hours at room temperature in ELISA plates (Nunc, Denmark) previously coated with 10 μg/mL of the monoclonal antibody L243 ...
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bioRxiv - Genomics 2021Quote: ... 2 wells of a 24 well plate were prepared and contained 2 mL RPMI-1640 media with 25 mM HEPES and L-glutamine (ThermoFisher Scientific) + 10% heat inactivated FBS (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher) was determined by AmplexRed assay [3] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1/512) and 50μl of each dilution was added to Immulon™ 2 HB flat bottom 96-well ELISA plates (Thermo Scientific) in triplicate ...
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bioRxiv - Neuroscience 2020Quote: Cortical primary neurons from newborn triple neurexin-1/2/3 conditional KO mice13 were seeded on Matrigel-coated coverslips in a 24-well plate in MEM medium (ThermoFisher Scientific). On DIV2 ...
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bioRxiv - Microbiology 2020Quote: ... and groups of 10 midguts were exposed to 2 × 106 parasites in 40uL of PBS in a well of an electron microscopy 9 cavity Pyrex pressed plate (Fisher Scientific). Chambers were transferred to a humidified chamber ...
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bioRxiv - Plant Biology 2022Quote: ... was used for floral dip transformation of pTAN1:CFP-TAN1 and selected on 1/2 MS plates containing 100 μg/mL gentamicin (Fisher Scientific). T1 seedlings were screened for mScarlet and CFP signal and then transferred to soil to self.
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bioRxiv - Microbiology 2020Quote: ... RNA was transfected into separate wells of a 24 well plate (2×105 cells; 500 ng RNA per well) using Lipofectamine 2000 (Thermofisher #11668019), media was changed at 3 h post transfection ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Microbiology 2021Quote: ... 6-well plate format) infected (MOI of 0.01) with WT or ΔORF rSARS-CoV-2 was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... replated at a density of 1.58 × 105 cells/cm2 in Vitronectin XF-coated 24 well plate into cardiomyocyte media (RPMI, 2% B27 supplement plus insulin [Life Technologies Australia ...
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bioRxiv - Cell Biology 2022Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 12 μl 10% 2-butanone (Acros Organics) in ethanol streaked across the lid with a pipette tip for 1 h ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... was cultivated in 24-well plates in complete OptiMEM® medium (OptiMEM® with 2% FBS, 0.5 µg/mL fungizone, and 40 µg/mL gentamicin, Gibco). After 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL of the start inoculum was cultivated in 2 mL of TSB liquid medium in a 24-well microtiter plate (Fisher Scientific). The microtiter plates were incubated statically at 30 °C for 24 h ...
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bioRxiv - Physiology 2019Quote: Control and VDR-KD cells were plated at 1.0 × 1010 cells/well in 2 ml of growth medium in 6-well plates (Nunc, Roskilde, Denmark). Both myoblasts and myotubes were maintained and harvested as described previously ...
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bioRxiv - Genomics 2019Quote: ... All plates were sealed with breathable membranes (Breathe-Easy) and incubated at 30°C in a thermostated incubator (Cytomat 2, Thermo Scientific) with continuous shaking ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was centrifuged at 2,000 × g for 1 min then lysate was transferred to a 384-well plate (30 μL × 2 TR-FRET replicates) using an adjustable electronic multichannel pipette (Matrix Equalizer, ThermoFisher 2231) and TR-FRET measurements were taken ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...