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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Oropouche Virus Gn Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Gn was detected with a polyclonal serum from a Gn-immunized rabbit (1:500 dilution, Thermo Fisher) as primary antibody ...
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bioRxiv - Microbiology 2019Quote: ... endoCP-GN and TSWV GN were cloned into pENTR/D-TOPO (Thermo Fisher Scientific, Grand Island, NY). TSWV GN was amplified by primers ENTR-endoCP-GNF and ENTR-TSWV-GNR1353 (Table S2) ...
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bioRxiv - Immunology 2022Quote: Glioma neural stem (GNS) cells were cultured in StemPro NSC medium (Thermo Fisher) as described previously26 ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Immunology 2023Quote: ... FAP-expressing GNS cells were first pre-labeled with CellTrace™ Far Red (CTFR; ThermoFisher) at 1μM for 20min at 370C ...
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bioRxiv - Immunology 2023Quote: ... GNS cell lines were cultured in StemPro Neural Stem Cell medium (StemPro NSC, ThermoFisher Scientific) in flasks ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Microbiology 2019Quote: ... vectors cloned with gene encoding Gn or Gc were transfected into HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA) using polyethylenimine ...
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bioRxiv - Biochemistry 2023Quote: Monolayers of HEK293T cells cultivated on coverslips were co-transfected with equimolar amounts of SIDT1/2-GN and SIDT1/2-GC plasmids using Lipofectamine 3000 (Invitrogen). After 24 hours of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... in the presence of 10 μg of purified Gn or NP antigens in 96 well V-bottomed culture plates (Nunc, Roskilde, Denmark). For intracellular detection of IFN-γ ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2020Quote: ... or P1 SARS-CoV-2/München- 1.1/2020/929 (Munich) virus was diluted to 500 µl in 15 ml with virus dilution medium (Opti-MEM, Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Next day cells were washed with phosphate-buffered saline (PBS) and infected with virus in Virus Production Serum Free Media (VPSFM; Gibco) supplemented with 0.25-0.5 ug/mL TPCK-Trypsin ...
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bioRxiv - Immunology 2019Quote: ... and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen). Amplification of cDNAs was performed by quantitative real-time PCR reactions on a Light Cycler instrument (LC480 ...
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bioRxiv - Microbiology 2020Quote: ... Virus was diluted in infection media (DMEM (Invitrogen) supplemented with 35% BSA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Microbiology 2020Quote: ... and 50% virus solution in RPMI media (Gibco). Mosquitoes were left to feed for 1.5 h using Hemotek membrane feeder system (Discovery Workshops ...
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bioRxiv - Immunology 2021Quote: ... virus was diluted in RPMI-1640 media (Gibco) and maintained on ice ...
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bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
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bioRxiv - Cancer Biology 2023Quote: ... then virus pellets were suspended in HBSS (Gibco). The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara) ...
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bioRxiv - Genomics 2023Quote: ... infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Genetics 2024Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Neuroscience 2023Quote: ... Virus particles were resuspended in Neurobasal A (Invitrogen) and stored at -80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...