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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... after which nuclear extracts were prepared with the Nucbuster protein extraction kit (Thermo Scientific, Rockford, USA). DNA binding capacity of the Nf-κB subunits relB and p52 were determined with the Nf-κB TransAM family kit (Active Motif ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellets were then lysed with cytoplasmic and nuclear extraction buffers according to manufacturer protocols (NE-PER Nuclear and Cytoplasmic Extraction Kit, Thermo Scientific). Protein concentrations of nuclear lysates were estimated using the BCA method outlined above ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Scientific) supplemented with protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagent kit (ThermoFisher) on ice at 4°C ...
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bioRxiv - Immunology 2023Quote: Nuclear lysates were prepared using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermofisher Scientific). Lysates were analyzed for total protein concentration using a BCA assay kit (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: Cytosolic and nuclear fractions were prepared using NEPER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagent kit (ThermoFisher) on ice at 4°C ...
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bioRxiv - Immunology 2022Quote: NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher) was used for nuclear extraction of AMs ...
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bioRxiv - Bioengineering 2024Quote: ... NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher, #78835) was utilized ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Nuclear fractions were isolated with a NE-PER Nuclear and Cytoplasmic Extraction Kit (78835, ThermoFisher Scientific), according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclear lysates were prepared using the NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear fractions were obtained using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The nuclear extracts were isolated using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific), as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... or cytoplasmic and nuclear extracts were isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher), each according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclear and cytoplasmic proteins of PSC were extracted according to the instructions of the nuclear-cytoplasm extraction reagents (Thermo Fisher Scientific, USA, Catalog number: 78835). Protein concentrations were quantified using Pierce protein assay kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... total protein was extracted using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents kit (78833, Thermo Fisher) and the cytoplasmic fraction used for western blots ...
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bioRxiv - Cancer Biology 2021Quote: ... NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher # 78833) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Thermo Fisher) was used according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... or using a nuclear/cytoplasmic extraction kit (Thermo Scientific 78835) to separate compartments ...
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bioRxiv - Microbiology 2020Quote: ... Cytoplasmic and nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagent kit (ThermoFisher) on ice at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... nuclear immunoprecipitations were performed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagent kits (Thermo Scientific™). Briefly ...
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bioRxiv - Genomics 2019Quote: Nuclear extracts from K562 cells were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (ThermoFisher). 70 base pair sequences containing either the reference alleles for rs2366739 and rs1194196 (referred to as TA ...
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bioRxiv - Molecular Biology 2020Quote: Nuclear and cytoplasmic fractions were prepared using the Nuclear and Cytoplasmic Extraction reagents (Thermo-Fisher Scientific, 78833) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Nuclear and cytoplasmic extracts were separated using NE-PER™ Nuclear and Cytoplasmic Extraction (Thermo Fisher Scientific) reagents as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclear and cytoplasmic extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Scientific, MA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The cytoplasmic and nuclear fractions were obtained with NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cytoplasmic and nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagent kit (ThermoFisher) on ice at 4°C ...
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bioRxiv - Immunology 2023Quote: Nuclear and Cytosolic protein fractions from cell cultures were extracted with NE-PER Nuclear and Cytoplasmic Extraction Reagents from Thermo Fisher (Cat. No. 78835, Waltham, MA, USA), and protein from whole cell fraction as well as supernatants were extracted with M-PER buffer from Thermo Fisher Scientific (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... nuclear protein was isolated using the NE-PER nuclear kit (Thermo Scientific), then proteins separated on 4-12% Bis-Tris Bolt gels using electrophoresis before transfer to nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Subcellular protein fractionations were performed using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The dry cell pellet was then processed for nuclear and cytoplasmic protein extraction as per manufacturer’s protocol (Thermo Scientific™NE-PER™ Nuclear and Cytoplasmic Extraction Reagents ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear-cytoplasmic fractionation was conducted using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Nuclear cell fractions were generated using the NE-Per™ nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... The cytosolic and nuclear extracts were prepared using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclear and cytoplasmic fractions were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific). Samples were separated by SDS-PAGE and then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2019Quote: Cytosolic and nuclear fractions were prepared using the NE-PER Nuclear and Cytosolic Extraction Reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cytoplasmic and nuclear fractions were separated using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, USA). The protein concentration of samples were estimated using Bradford Assay (Bio Rad ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were obtained using to the NE-PER™ Nuclear and cytoplasmic Extraction reagents kit (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: The nuclear and NE fractions were prepared using the NE-PERTM Nuclear and Cytoplasmic Extraction kit (Thermo Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cytoplasmic and nuclear fractionation was performed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific #PI78835). The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222 ...
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bioRxiv - Biochemistry 2020Quote: ... Cytoplasmic and nuclear fractions were prepared from lysates using Nuclear and Cytoplasmic Extraction Reagent (Thermo Fisher Scientific, USA). Cytoplasmic or nuclear fractions were loaded on 8–15% polyacrylamide gels and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclear and cytoplasmic extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cytoplasmic and nuclear fractionation was performed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific #PI78835). The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222 ...
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bioRxiv - Cancer Biology 2019Quote: Nuclear and cytoplasmic fractions were separated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear and cytoplasmic THP-1 fractions were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher) according to instructions ...
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bioRxiv - Immunology 2020Quote: ... Nuclear-cytoplasmic fractionation was conducted using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear lysate from VCaP or LNCaP cells were extracted using the NE-PER nuclear extraction kit (Thermo Scientific) and dialyzed against the FPLC buffer (20 mM Tris-HCl ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: Nuclear extracts were prepared with the NE-PER Nuclear and Cytoplasmic Extraction Kit (78833, Thermo Scientific™, USA) following the protocol provided by the manufacturer ...