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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... while acetylcholine receptors were detected by a-bungarotoxin (Alexa Fluor 488®, Invitrogen # B13422). Nuclei were stained with 300 nM DAPI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-NMDA receptor subunit 1 (1:1000, ThermoFisher PA3-102), and chicken anti-tyrosine hydroxylase (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-oestrogen receptor alpha (Thermo Fisher Scientific, clone SP1), anti-progesterone receptor (DAKO ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells stably expressing the ecdysone receptor (EcR-293; Invitrogen), and derived cell lines stably expressing GFP-tagged wild-type or dominant negative (E228Q ...
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bioRxiv - Neuroscience 2023Quote: ... Acetylcholine (Fisher Scientific) was bath-applied with physostigmine hemisulfate (eserine ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-CaMKII alpha subunit (clone 6G9, Thermo Scientific, #MA1-048, 1:1000), mouse monoclonal anti-NaV1.8 (clone N134/12 Neuromab Facility ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney (HEK293, Thermo Fisher Scientific) cells ...
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bioRxiv - Cell Biology 2022Quote: Human HEK293 cells were cultured at 37 °C and 5% CO2 in DMEM (ThermoFisher Scientific), supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Live-cell imaging to show mature neuronal response to potassium chloride (KCl) or acetylcholine (ACh) was performed using Fluo-4-AM (Invitrogen™, F14217). Cells were incubated with Fluo-4-AM in HBSS-Ca2+ (45 min ...
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bioRxiv - Immunology 2021Quote: ... or with an antibody against the nicotinic acetylcholine receptor ɑ7 (nAChr ɑ7; Alomone Labs) followed by an Alexa594 goat-anti-rabbit conjugate (Invitrogen). Nuclear counterstain with Hoechst 33342 (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Increasing doses of acetylcholine (Acetylcholine chloride, 99%, Thermo Fisher Scientific; Waltham, MA, USA) ranging from 10−9 to 10−4 M were administered sequentially through the pressurized luminal loop to probe for endothelium-dependent vasodilatation ...
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bioRxiv - Microbiology 2020Quote: ... and the Human embryonic kidney (HEK293) cells (Gibco) in Freestyle F17 expression medium (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Human derived HEK293 cells (Invitrogen, Inchinnan, UK, R75007) stably transfected with cAMP GloSensor™ (pGloSensor-22F ...
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bioRxiv - Neuroscience 2023Quote: Grip TiteTM Human Embryonic Kidney (GT HEK293) (Invitrogen) cells were grown as previously described (Chałupnik et al ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... acetylcholine (Fisher Scientific cat# AC159170050), oxotremorine-M (Santa Cruz Biotechnology cat# sc-203656) ...
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bioRxiv - Developmental Biology 2021Quote: ... 35 μm thick sections were used immunohistochemistry (IHC) of acetylcholine receptors (α-Bungarotoxin extracted from Bungarus muticinctus, conjugated to Alexa Fluor 647, Thermofisher #B35450) and nerve fibers (chicken anti-neurofilament-H antibody ...
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bioRxiv - Neuroscience 2023Quote: Human Embryonic Kidney Grip TiteTM (HEK293-GT) cells (Invitrogen) was used to create a polyclonal cell line expressing GluA2(Q)i as previously described [49] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mutations introduced into the δ-subunit were established as stable HEK293 Flp-InTM cell lines (Invitrogen, Paisley, UK), generating a stable cell line for each mutant ...
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bioRxiv - Cancer Biology 2021Quote: ... and Acetylcholine chloride (Ach) (Invitrogen, US) in doses as indicated in the text were given in normoxia or 0.2% hypoxia.
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bioRxiv - Pharmacology and Toxicology 2021Quote: The human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... 5% CO2 and 37°C in neuronal culture medium (Gibco Neurobasal medium supplemented with 1x Gibco B-27 supplement ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human embryonic kidney cell lines (HEK293) containing a Flp-In expression vector (HEK293 Flp-In) obtained from ThermoFisher Scientific were utilized in this study ...
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bioRxiv - Immunology 2022Quote: ... and Hi-5 cells cells (Invitrogen), respectively ...
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bioRxiv - Cancer Biology 2021Quote: Acetylcholine concentration in cell lysate was detected using Amplex® Red Acetylcholine/ Acetylcholinesterase (AChE) Assay Kit (Invitrogen, US) using the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... then incubated with human Fc receptor coupled to phycoerythrin (ThermoFisher). To set positive gates ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse anti-human transferrin receptor antibody was from Life Technologies. Secondary IRDye-labeled goat anti-mouse and anti-rabbit IgG antibodies were from LI-COR Biosciences ...
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bioRxiv - Neuroscience 2022Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used as per the manufactures instructions to quantify soluble IL-6R expression in day 14 MGL and day 18 NPC vehicle/treated cell culture media ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used to quantify soluble IL-6Ra expression in cell culture media ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... or Human TNF alpha ELISA Ready-SET-Go! (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... or Human TNF alpha ELISA Ready-SET-Go! (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the human δ-subunit into the pcDNA5/FRT vector (Invitrogen, Paisley, UK) using the δ-construct described previously (28) ...
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bioRxiv - Biophysics 2021Quote: ... Human embryonic kidney 293 cells (HEK293) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) were grown in DMEM/F12 media (GIBCO) supplemented with HyClone cosmic calf serum (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney (HEK293) cells were cultured in DMEM (Thermofisher Scientific) containing 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Human Embryonic Kidney cells (HEK293-CD19+) were cultured in DMEM (Gibco), 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... TNF-alpha treatments were performed for 45 min using 25 ng/ml of human TNF-alpha (Thermo Fisher). Cells were permeabilized with 0.1% Triton X in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibody dilutions were used: anti-folate receptor alpha 1 (1/300; Thermofisher, ref: PA5-101588) for nuclear fraction and anti-Sox2 (1/1000 ...
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bioRxiv - Biophysics 2021Quote: ... The human Gβ1 and Gγ2 subunits were integrated into the pFastBac Dual vector (Invitrogen) with an N-terminal 6 × His-tag at the N terminus of Gβ1 ...
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bioRxiv - Immunology 2021Quote: Human TNF alpha Uncoated ELISA Kit (ThermoFisher, catalog no. 88-7346) was used to determine secretion of TNF-α in culture supernatants of cells treated with SARS-CoV-2 S1 subunit ...
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bioRxiv - Cell Biology 2024Quote: ... and human GRO alpha (CXCL1) Uncoated ELISA (88-52122-22, Invitrogen). Collagen type II synthesis ...
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bioRxiv - Genetics 2020Quote: HEK293 and human fibroblast culture medium consisted of DMEM (Gibco; 11995-065) supplemented with 10% fetal bovine serum (Gibco 16000-036) ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK293; Invitrogen’s Flp-In-293 cell line cat #R75007) cells were grown and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...