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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Nestin NES Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... 100 mg of tissue was homogenized using 10 strokes in a 2 ml ground glass dounce-type tissue grinder in the buffers provided in the NE-PER nuclear and cytoplasmic extraction kit from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Nuclear and cytoplasmic fractions from day 21 MSC pellets were separated with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... TDP-43 CRISPR KO HeLa cells were lysed for N/C fractionation and SDS-PAGE with the NE-PER kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: In order to separate between nuclear and cytoplasmic fractions we used NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) or hypotonic fractionation protocol (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear and cytoplasmic fractions were isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, Waltham, MA, USA). The circRNAs were reverse-transcribed using PrimeScriptTM reagent Kit with DNA Eraser (TaKaRa BIO ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with Halt protease inhibitor cocktail [Thermo Fisher Scientific]) to obtain whole-cell lysates or lysed using the NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific) to obtain cytoplasmic and nuclear fractions ...
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bioRxiv - Cancer Biology 2022Quote: Cells were fractionated into cytoplasmic and nuclear fractions using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific) according to the product manual.
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bioRxiv - Genomics 2019Quote: ... Nuclear extracts were prepared from actively growing melanoma cells (UACC2331) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in PBS and fractionated using the NE-PER™ Nuclear and Cytoplasmic Extraction Kit following the manufacturer’s protocol (ThermoFisher).
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bioRxiv - Bioengineering 2019Quote: ... New England Small Tube NE-1310-02) and Tygon ND-100-80 tubing (O.D. 0.06 in, I.D. 0.02 in; Fisher Scientific 14-171-284) to a control manifold ...
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bioRxiv - Cell Biology 2020Quote: The nuclear and cytosolic protein lysates were prepared by using the NE-PER Nuclear and Cytoplasmic Extraction kit (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Nuclear and cytoplasmic fractions of mHat9d cells were performed using the NE-PER Nuclear and Cytoplasmic Extraction reagents (Thermo Scientific), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were obtained from pelleted cells with the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, 78833) following instructions from the manufacturer and using protease and phosphatase inhibitors (Millipore ...
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bioRxiv - Immunology 2022Quote: ... Nuclear and cytoplasmic extracts from CD8+ T cells were obtained with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, ThermoFisher). The Revert 700 Total Protein Stain (LICOR ...
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bioRxiv - Immunology 2023Quote: ... Cytoplasmic and nuclear fractions were harvested using with NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, #78833) and evaluated by NF-κB (Cell Signaling ...
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bioRxiv - Genetics 2023Quote: ... was extracted from skin fibroblasts derived from two different Welsh Mountain ponies that were overexpressing human SOX11 (as described above) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... mouse liver tissue or HepG2 cells were separated with NE-PER Nuclear and Cytoplasmic Extraction Reagents (#78833, Thermo Fisher Scientific) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear and cytoplasmic proteins were prepared using the NE-PER nuclear and cytoplasmic extraction reagent kit (Thermo Scientific™, 78833). Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB ...
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bioRxiv - Cancer Biology 2022Quote: ... subcellular protein extracts were obtained by using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Cat #78833, Thermo Fisher Scientific). Transfected cells were washed with PBS and trypsinized for 1 min using Trypsin-EDTA solution (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were subjected to lysis using NE PER Nuclear and Cytoplasmic extraction kit (Thermo Fisher Scientific Inc, Grand Island, NY) and proteins were extracted following manufacturer guidelines.
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bioRxiv - Immunology 2023Quote: AML and MDM cells were lysed with NE-PER Nuclear and Cytoplasmic Extraction kit according to the manufacturer’s protocol (Thermo Scientific). Western blot was performed and the membranes were incubated with the primary antibody for PPAR-γ ...
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bioRxiv - Genetics 2023Quote: ... with 8ug of LCL nuclear extract prepared using the NE-PER Nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific, 78833) and 30 ng of recombinant purified ELK1 (Sino Biologicals 12621-H20B) ...
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bioRxiv - Physiology 2024Quote: The separation of nuclear and cytoplasmic protein analysis was performed using NE-PER nuclear and cytoplasmic extraction kit (Invitrogen #78835). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Lumbar spinal cords were dissected in oxygenated artificial cerebrospinal fluid and immediately homogenized using Cytoplasmic Extraction Reagent Kit from the NE-PERTM nuclear extraction kit (ThermoFisher) with a protease inhibitor cocktail added (5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Nkx2-1 and Pax8 and bTg-NES-BrafV637E-ERT2 sequences (Supplementary Fig. 1A) following the lipofectamine 3000 protocol (Thermo Scientific). The BrafV637E mutation here used for mouse cells is equivalent to the human BRAFV600E mutation56 ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclear and cytoplasmic protein fractions were obtained using an NE-PER™ nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cytoplasmic and nuclear portion of protein were extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific, USA). Protein concentration was measured using the Bradford assay (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2021Quote: Nuclear and cytoplasmic cell fractionation was prepared using NE-PER Nuclear and cytoplasmic extraction kit from Thermo Scientific (Cat no.78833) and manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell extracts obtained from 2×105 cells with an NE-PER Nuclear and Cytoplasmic Extraction kit (Thermo Fisher Scientific, 78833) were loaded into a 12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellets were then lysed with cytoplasmic and nuclear extraction buffers according to manufacturer protocols (NE-PER Nuclear and Cytoplasmic Extraction Kit, Thermo Scientific). Protein concentrations of nuclear lysates were estimated using the BCA method outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Final proteins concentration in the NE extracts was determined using the colorimetric Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA). Pellets were stored at −80° C.
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bioRxiv - Cancer Biology 2020Quote: ... the cells were harvested and subjected to nuclear and cytosolic fractionation via NE-PER kit (ThermoFisher Scientific; Cat# 78833, Waltham, MA). Nuclear protein was isolated and 100 μg of lysate was submitted to UAMS proteomics core facility.
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bioRxiv - Neuroscience 2021Quote: Nuclear fractions from ipsilateral sciatic nerve injury sites from each rat were isolated with a NE-PER Nuclear and Cytoplasmic Extraction Kit (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... wtCCDC170 or the ΔCCDC170 fusion variants E2-E7 or E2-E10 was performed using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Subcellular fractionalization and lysate preparation were carried out using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher, catalog number 78833). Lysate protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: Nuclear and cytoplasmic fractions were extracted from cells using NE-PER™ Nuclear and Cytoplasmic Extraction Reagent kits (Thermo Scientific™). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... dCas9-N57-and N57-dCas9-transfected HeLa cells were generated using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) according to manufacturer’s instructions and total protein concentration was determined by Bradford assay ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear and cytoplasmic cell fractionation was prepared using NE-PER Nuclear and cytoplasmic extraction kit from Thermo Scientific (Cat no.78833) and manufacturer’s instructions were followed ...
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bioRxiv - Neuroscience 2019Quote: ... Stepwise separation of cytoplasmic and nuclear protein extracts were prepared by NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific, 78833) by following manufactures instructions ...
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bioRxiv - Genetics 2021Quote: ... We used approximately 16 ug of nuclear protein extract from Capan-1 cells purified using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) per binding reaction.
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bioRxiv - Cell Biology 2021Quote: Nuclear and cytoplasmic fractions were collected from tissue culture samples and dKO tissues using the NE-PER™ kit as described in manufacturer’s instructions (ThermoFisher Scientific). Samples were run on 10% Bis Tris gels (Thermo Scientific Technologies) ...
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bioRxiv - Microbiology 2022Quote: Differentiated LT-treated and control enteroid monolayers were lysed to using NE-PER™ nuclear and cytoplasmic extraction reagent (Thermo Scientific). Cell membrane pellets were solubilized in PBS containing 1% Triton X-100 supplemented with protease inhibitor cocktail (Pierce™ protease inhibitor mini ...
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bioRxiv - Microbiology 2022Quote: ... MYXV infection or Selinexor + MYXV and nuclear and cytosolic fractions were prepared using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific). Purity of the fractions was confirmed by western blot analysis for tubulin (cytoplasmic ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear soluble and chromatin fractions were prepared and combined as nuclear extracts (NEs) using a Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific). All experiments were performed at least twice.
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bioRxiv - Systems Biology 2022Quote: The whole kidney was homogenized and nuclear fraction was extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific, # 78833), following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: Western blot analysis was conducted using cytoplasmic extracts from mouse brain cells made with a NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher). The mixture was centrifuged at 10,000□×□g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear and cytosolic protein fractions from muscle biopsies were prepared using NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Catalog. No 78835; ThermoFisher) according to the manufacturer instructions with the help of Dounce homogenizer.
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bioRxiv - Neuroscience 2022Quote: Cortex from 3-4 months mice brains were dissected and homogenized (pool of three animals) in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
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bioRxiv - Cell Biology 2023Quote: Pull-down experiments were performed as detailed in Wysocka [52] after nuclear extraction with NE-PER commercial kit following manufactureŕs instructions (Thermo Scientific #78833) and run in SDS-PAGE for a short time in order to avoid separation ...
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bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...