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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... After blocking with 4% horse serum (Gibco) in PBST ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Acidified peptides quantified using Qubit 4 Fluorometer (ThermoFisher). All following spin steps occur at 1,600 x g unless otherwise noted ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or pCDNA3-NKX2-174 using Lipofectamine 3000 (Thermo Fisher Scientific, L3000075). Cells were lysed 48 hours post-transfection with Dynabeads Co-Immunoprecipitation kit lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... then washed 4 times and eluted with HA peptide (ThermoFisher) at 37°C for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Blocking was carried out overnight at 4°C in a humidified dark chamber using Blocking Aid Solution (Thermo Fisher Scientific). Primary antibody diluted in Blocking Aid Solution (Anti-PvGAMA 1:1200 ...
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bioRxiv - Biochemistry 2019Quote: ... 4 pmol unlabeled CysOx Peptide Standard (VSAPGAGSAKADTGPACGTAR, custom synthesized by ThermoFisher) was added to each sample for normalization ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated with 4% ECL Blocking Agent (Fisher Scientific # 45001197) prepared in 1x TBST buffer (20 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... Antibodies were NKX2-5 (mouse, R&D, 1:500) and β -actin (rabbit, Invitrogen, 1:1000). Subsequently ...
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bioRxiv - Biochemistry 2019Quote: Peptides were loaded onto a precolumn (4 cm POROS 10R2, Applied Biosystems) and eluted with an HPLC gradient (NanoAcquity UPLC system ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4 °C in blocking solutions and secondary antibody (Invitrogen, 1:500) for 1-2 hr at room temperature ...
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bioRxiv - Physiology 2022Quote: ... The membranes were blocked with 4% StartingBlock T20 Blocking Buffer (Thermo Fisher Scientific) with 0.1% Tween-20 in PBS or TBS for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the A2Lox.Cre_TRE-Nkx2-1/Pax8_Tg-EGFP mouse ESCs (Romitti, 2021) were transfected using Lipofectamine 3000 reagent (Invitrogen), and after 24 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Bioengineering 2020Quote: ... This solution (10% blocking buffer in PBS) contained 4 µM Hoechst 33342 (Invitrogen H3570) and 6.6 µM phalloidin AF488 (Invitrogen A12379) ...
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bioRxiv - Physiology 2021Quote: ... Secondary antibodies (goat anti-rabbit-Alexa Fluor555, 4 μg/ml in blocking buffer; Invitrogen) were then added for 1 h at room temperature followed by DAPI DNA Stain (1 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... peptides were desalted for 4 minutes on a hand-packed trap column (Thermo Scientific POROS R2 reversed-phase resin 1112906 ...
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bioRxiv - Cell Biology 2020Quote: Peptides were loaded onto a precolumn (100 µm × 4 cm POROS 10R2, Applied Biosystems) and eluted with an HPLC gradient (NanoAcquity UPLC system ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were desalted for 4 minutes on a hand-packed trap column (Thermo Scientific POROS R2 reversed-phase resin 1112906 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides from each fraction were loaded onto a precolumn (4 cm POROS 10R2, Applied Biosystems) and eluted with an HPLC gradient (NanoAcquity UPLC system ...
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bioRxiv - Cancer Biology 2020Quote: ... The peptides were then labelled with TMT reagent (1:4; peptide:TMT label)(Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... and blocked by using blocking solution (PBS plus 4% Bovine serum albumin, fraction V (Fisher Scientific) supplemented with 1% goat serum) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were blocked for 30 minutes at 4°C in SEA Block blocking buffer (37527, ThermoFisher. Primary antibodies were diluted in 10mL SEA Block ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated 2hrs at 4°C with secondary antibody (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Bioengineering 2023Quote: ... The device was incubated for 1 hour at room temperature in 0.4 μL of Alexa Fluor 594 donkey anti-rabbit (for anti-NKX2-5, Life Technologies) and Alexa Fluor 488 donkey anti-mouse (for anti-TNNT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nkx2-1 and Pax8 and bTg-NES-BrafV637E-ERT2 sequences (Supplementary Fig. 1A) following the lipofectamine 3000 protocol (Thermo Scientific). The BrafV637E mutation here used for mouse cells is equivalent to the human BRAFV600E mutation56 ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide samples were dissolved in 0.1% (v/v) formic acid (FA, Thermo Fisher Scientific, LS118-4) for MS analysis ...
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bioRxiv - Cell Biology 2022Quote: ... and then blocking in Superblock Blocking Buffer (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed using BlockAid Blocking Solution (Thermo Fisher Scientific). Primary antibody incubation against clathrin heavy chain (ab21679 from Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Blocking Buffer (Invitrogen Cat# 00-4952-54 ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... ~4 mg of peptides were enriched for phosphopeptides using a Fe-NTA phosphopeptide enrichment kit (Thermo Scientific) according to instructions provided by the manufacture and desalted ...
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bioRxiv - Cell Biology 2022Quote: ... The digested peptides were labelled with 0.2 mg per reaction of 4-plex TMT reagents (ThermoFisher scientific) (126 ...
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bioRxiv - Developmental Biology 2022Quote: ... SYBR Green-based qRT-PCR was performed for SHF (Isl1, Tbx1, Mef2c, Fgf10, Hand2), and CM (Nkx2-5, Tbx20, Myl2, Cited2) markers using StepOnePlus™ Real-Time PCR System (Applied Biosystems). Mean relative gene expression was calculated after normalizing Ct values to Gapdh using the ΔΔCt method and presented as Log10(Fold change) ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue sections were fixed in 4% paraformaldehyde for 15 min and blocked with universal blocking solution (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... All the digested peptides were labelled withiTRAQ 4-plex and purified using C-18 spin columns (Thermo Scientific). Labelled and purified peptides were analysed using LC-MS/MS OrbitrapVelos Nano analyser (Thermo Scientific ...