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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for N 3R Pyrrolidin 3 ylmethyl acetamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... acetamide (Thermo Scientific Chemicals), 1,3-dihydroxyacetone (VWR) ...
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bioRxiv - Physiology 2020Quote: ... 0.05% 3-(N,N-dimethylmyristylammonio) propanesulfaonate Zwittergent detergent (Acros Organics) in 1% methanol ...
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bioRxiv - Bioengineering 2020Quote: ... N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide HCl (50-848-678, Fisher Scientific), butyric acid (B103500-100ML ...
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bioRxiv - Physiology 2020Quote: ... 0.05% 3-(N,N-Dimethylmyristylammonio) propanesulfonate zwittergent detergent (Acros Organics, 427740050)) containing protease inhibitor (Mammalian ProteaseArrest-APExBIO K1008) ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from control (n=3) and model (n=3) Huh7 cells were isolated by TRIZOL reagent (Thermo Scientific), and the RNA concentration ...
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bioRxiv - Developmental Biology 2020Quote: RNA from Rbx2 fl/fl (n=3) and Rbx2cKO-Nes (n=3) P1 telencephalons was extracted using TRIzol (Invitrogen). Strand-specific and barcode-indexed RNA-seq libraries were generated from 1 μg total RNA each after poly-A enrichment using the Kapa Stranded mRNA-seq kit (KapaBiosystems ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Molecular Biology 2023Quote: ... HuH6 hCLDN1 [13] and Lunet N#3 and Lunet N#3 hCD81 [18] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Thermo Fischer Scientific) supplemented with 1 % MEM non-essential amino acids (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... 3-(N-maleimidylpropionyl)biocytin (MBP) was obtained from Invitrogen and the HRP-conjugated antibody from eBioscience ...
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bioRxiv - Biochemistry 2020Quote: ... N-cyclohexyl-3- aminopropanesulfonic (CAPS) was from Acros Organics, 2-(N-Morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Synthetic Biology 2020Quote: ... N-cyclohexyl-3-aminopropanesulfonic (CAPS) was from Acros Organics, 2-(N-Morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Cell Biology 2023Quote: ... The carboxyl groups on the bead surfaces were functionalized with amine-reactive groups via N- ethyl-N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and sulfo-N-hydroxysuccinimide (sulfo-NHS, Thermo Fisher) crosslinking for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Tissue sections from male (n = 3) and female (n = 3) rats were mounted on subbed glass slides (Fisher brand Superfrost Plus, Fisher Scientific, Hampton, NH, USA) and desiccated overnight (~16 h ...
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bioRxiv - Genomics 2022Quote: ... 3 μM CHIR99021 (Stemgent) and 0.5× N-2 Supplement (Gibco)) which was changed daily for the cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’-GAAGUCACAACAGAGUGCAGGCAAA-3’) and an appropriate control (Cat. N° 12935300 - Invitrogen) using Lipofectamine RNAiMAX reagent (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from gastrocnemius muscles of experimental mice (n=4 BC-PDOX; n=4 BC-PDOX PIO; n=3 NSC-Con) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods21 ...
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bioRxiv - Biophysics 2023Quote: ... 3-(N-morpholino)propanesulfonic acid) (MOPS) was purchased from Acros Organics. Texas Red DHPE (TR-DHPE) ...
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Northern blots were performed as previously described with infrared probe and EDC (N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide) (Thermo Scientific) crosslinking43 ...
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bioRxiv - Biochemistry 2023Quote: ... and Glycerol-3-Phosphate (cat. N° 20729) were from Cayman. Schneideŕs Drosophila medium (cat. N° 21720024) was from Gibco. ADP ...
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bioRxiv - Cell Biology 2023Quote: ... 2- & 3-methyl pentane and n-hexane (Thermo Scientific, Waltham, MA, USA). Reported compounds detected by the GC-MS were confirmed by matching retention times and mass–charge (m/z ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) and Sulfo-NHS (N-hydroxysulfosuccinimide) were purchased from ThermoFisher (USA). Unless otherwise stated all other chemicals were received from Sigma (St Louis ...
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bioRxiv - Microbiology 2021Quote: ... N gene reverse primer (5’-GAGGAACGAGAAGAGGCTTG-3’) and probe (5’-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3’) using Taqman mastermix (Thermo Fisher). The thermal cycling steps were ...
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bioRxiv - Neuroscience 2020Quote: ... and goat anti-rabbit Alexa 647 (Invitrogen, Waltham, MA; for N = 3 birds). After 3 more washes in 0.1 % PBT ...
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bioRxiv - Pathology 2022Quote: ... VSMCs (n = 3) were lysed using Pierce immunoprecipitation lysis buffer (Thermo Scientific, 87788) supplemented with protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2021Quote: RNA samples were extracted from mESCs (n = 3 biological replicates) using Trizol reagent (Invitrogen) and subjected to DNase digestion with Turbo DNase (Ambion AM2238) ...
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bioRxiv - Biophysics 2020Quote: ... each sample was double-blinded microinjected (n=3) with fluorescein-labeled dextran (Life Technologies) in 100-200 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... pooled (n>3 embryos for each genotype) and digested with 0.25% Trypsin/EDTA (Invitrogen), neutralized in DMEM (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 16 μL N,N,N’,N’-tetramethylethane-1,2-diamine (TEMED) (Fisher Scientific), 300 μL ammonium persulfate (APS ...
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bioRxiv - Cell Biology 2022Quote: ... TEMED (N,N,N′,N′-tetramethylethylenediamine; cat. no. J63734.AC, Thermo Scientific) to polymerize the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Pierce premium grade N-hydroxysulfosuccinimide (sulfo-NHS, PG82071) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 22980) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Genomics 2024Quote: ... liver and muscle tissues from a total of 72 fish (n = 8 salmonid species x 3 biological replicates x 3 tissues) using TRIzol (Invitrogen/Life Technologies) with glass mill beads (1mm ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl) ethylenediamine (Fisher Scientific, AAL16280AP) and 15 wt% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.5 μl of TEMED (N,N,N’,N’-tetramethylethylenediamine; 15524-010, Invitrogen). A 8 μl drop was quickly deposited onto each 19 mm coverslip ...
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bioRxiv - Developmental Biology 2024Quote: ... 25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl) ethylenediamine (Fisher Scientific, AAL16280AP) and 15 wt% Triton X-100 ...
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bioRxiv - Physiology 2023Quote: EAT (occluded and non-occluded tissue pooled) from exercise-trained (n=4) and sedentary (n=3) female pigs had total RNA extracted with TRIzol® (Thermofisher Scientific, Waltham, MA). Total RNA was quantified (Nanodrop 3300 ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... four 6.5 µL aliquots of the purified arrays were moved into the interim pDonor4r-3r in parallel by the standard Invitrogen Gateway BP reaction recipe (ThermoFisher 11789-020) which was allowed to remain at room temperature for 72 h prior to stopping the reactions with Proteinase K and transformation of all material into E ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Genomics 2024Quote: ... liver and muscle tissues from a total of 72 fish (n = 8 salmonid species x 3 biological replicates x 3 tissues) using TRIzol (Invitrogen/Life Technologies) with glass mill beads (1mm ...