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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Myelin protein P0 MPZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... rabbit anti-keratin 14 (KRT14, RB-9020-P0, Thermo Fisher, Waltham, MA; 1:500), rat anti-mouse CD68 (FA-11 ...
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bioRxiv - Neuroscience 2021Quote: Eyes from P0-1 Glast-CreER;R26R-EYFP mice were collected in DPBS (Gibco) under sterile conditions ...
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bioRxiv - Neuroscience 2020Quote: Mouse hippocampi from C57BL/6J P0 pups were dissected in ice-cold HBSS (Gibco) and digested with papain (Worthington ...
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bioRxiv - Biochemistry 2022Quote: ... and transferred onto a nitrocellulose membrane using an iBlot2 device (P0 program, Life Technologies). After probing for total transferred protein using Ponceau S (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampi from P0 - P1 SD rats were dissected in Hank’s balanced salt solution (Invitrogen), digested with papain (Worthington ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: HEK293 Flip-In TREx cells (Thermo Fisher) were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher ...
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bioRxiv - Biochemistry 2020Quote: HEK293-F cells (FreeStyle™, ThermoFisher #R79007) were maintained in suspension culture according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T or HEK293 GnTI- cells (Thermo Fisher) were incubated with 200 nM (monomer concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Flp-In T-REx 293 (Invitrogen), was grown at 37°C in DMEM high glucose supplemented with 5% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HEK293-Flp-In T-Rex cells (Invitrogen) were cultured in high glucose DMEM containing 10% FBS at 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells grown in DMEM media (Gibco) supplied with 10%FBS and 2mM L-Glutamine were seeded onto coverslips in 24 well plates and allowed to adhere and grow overnight at 37°C incubator supplied with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293-FT cells were purchased from Invitrogen. U87MG cells were Purchased from the ATCC and cultured as previously described (Sabag et al. ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells was cultured in DMEM (Invitrogen) supplemented with 10% FBS at 37 °C and 5% CO2 environment ...
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bioRxiv - Cancer Biology 2022Quote: ... Flp-In T-REx HEK293 cells (ThermoFisher) were cultured in DMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In 293 cell line (HEK293; ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2023Quote: HEK293 cells (Thermo Fisher, Waltham, MA, USA) were cultured at 37°C in Dulbecco’s Modified Eagle’s Medium containing high levels of glucose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in DMEM (Gibco) containing penicillin (100 U/mL ...
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bioRxiv - Genetics 2024Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% FBS (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: Flp-InTM T-RexTM HEK293 (Thermo Fisher), Flp-InTM T-RexTM HeLa (gift from S ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... P0-1 postnatal mouse cortical or hippocampal neurons were maintained in Neurobasal-A Medium (Gibco) supplemented with 2% B-27 ...
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bioRxiv - Neuroscience 2020Quote: ... Microglial cells were separated from myelin/debris by Isotonic Percoll gradient centrifugation (35% Fisher Scientific) at 2000 rpm for 45min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Sections were stained for myelin using the BrainStain Imaging kit (ThermoFisher #B34650; FluoroMyelin, 1:300), following the vendor protocol.
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...