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Citations for Thermo Fisher :
151 - 200 of 1454 citations for Mumps Virus Nucleoprotein Strain Jeryl Lynn since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 nucleoprotein cDNA was generated from RNA from Bei Resources (NR-52285) by One-Step RT PCR (SuperScript IV, Thermo Fisher) with primers SARS COV-2 N IVT F1 (5’-GAATTCTAATACGACTCACTATAGGGGATGTCTGATAATGGACCC-3’ ...
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bioRxiv - Immunology 2021Quote: ... against the IAV nucleoprotein (forward: 5’-CAGCCTAATCAGACCAAATG-3’; reverse: 5’-TACCTGCTTCTCAGTTCAAG-3’) were assayed using SYBR Green Master Mix (Applied Biosystems) to confirm viral presence in the maternal lung.
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bioRxiv - Microbiology 2022Quote: ... For determination of infection rates employing wide field fluorescence microscopy the following antibodies in combination with a DAPI counter stain were used: mouse anti-nucleoprotein/goat anti-mouse Alexa488 (ThermoFisher Scientific). Cells grown on cover slips were mounted on microscopy slides using Mowiol.
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was detected by virus-specific primer/probe according to indicated reference sequences (Table 1) utilizing TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems) and QuantStudio™ 5 (ThermoScientific ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... coli K-12 strain Bioparticles (Thermo Fisher, Alexa Fluor 488 conjugate Ex/Em = 495/519 ...
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bioRxiv - Microbiology 2020Quote: ... coli strain DH5α for genetic manipulations (Invitrogen). The B ...
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bioRxiv - Plant Biology 2019Quote: ... cerevisiae yeast strain (INVSc1 from ThermoFisher Scientific) with each plasmid DNA ...
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bioRxiv - Synthetic Biology 2020Quote: The Pichia pastoris GS115-strain (Life technologies) was used for protein expression and is the starting strain for glyco-engineering ...
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bioRxiv - Bioengineering 2021Quote: ... coli TOP10 strain (Thermo Fisher Scientific, USA) was used for gene cloning ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21AI strain (Thermo Fisher Scientific Inc.). The transformants were grown in shake-flask culture at 30°C in Lysogeny Broth (LB ...
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bioRxiv - Biophysics 2021Quote: ... coli strain BL21(DE3)Star/pCodonPlus (Invitrogen). The cultures were grown to OD600 = 0.8-1.0 and expression was induced with 0.5 mM IPTG at 25°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strain DH5α (Thermo Fisher Scientific) was used for cloning ...
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bioRxiv - Neuroscience 2020Quote: ... using the Escherichia coli strains DH5α (Invitrogen) and XL1-Blue (Stratagene) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH10B strain (Invitrogen, suppl. table 2) was used for expression of the synthetic operon ...
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bioRxiv - Pathology 2019Quote: The Escherichia coli strain Top10 (Life Technologies) and Saccharomyces cerevisiae strain BY4743 were used for cloning purposes ...
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bioRxiv - Molecular Biology 2019Quote: Agrobacterium tumefaciens strain LBA4404 (Invitrogen, Carlsbad, CA) electro-competent cells were transformed with pMHK-98 through electroporation method as described previously (Asad et al ...
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bioRxiv - Microbiology 2020Quote: ... coli strains DH5α and BL21 (DE3) (Invitrogen) were used for cloning and BclA3CTD overexpression ...
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bioRxiv - Genetics 2021Quote: ... coli expression strain (Thermo Fisher, Waltham, MA). Mid-log phase cells were induced with 1 mM IPTG (Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... cerevisiae strain INVSc1 (Thermo Fisher Scientific Inc.) and plated out onto uracil-free (Ura- ...
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bioRxiv - Molecular Biology 2021Quote: ... coli strains MAX Efficiency™ DH5αF’IQ (Invitrogen) and NEB® 5-alpha F’IQ (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... PichiaPink™ yeast strain one (Invitrogen, USA) was grown according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Escherichia coli strain TOP10 (Thermo Fisher Scientific) was used employing standard methods and enzymes purchased from Thermo Fisher Scientific or New England BioLabs (NEB).
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain DH5-alpha (Invitrogen, 18265-017) was used for molecular cloning ...
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bioRxiv - Microbiology 2023Quote: ... coli strain Mach1 2T1 (Invitrogen, Waltham, MA). E ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain One Shot™ TOP10 (Invitrogen) for plasmid amplification or BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain (Thermo Fisher Scientific, Waltham, MA), which were then cultured in LB Miller Broth (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... coli BL21 (DE3) Rosetta strain (Invitrogen, Germany). Following induction of GST-TTN5 fusion protein expression according to standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... cerevisiae (Strain INVSc1, Thermo Fisher, Cat#: C81000) was used for protein expression ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain BL21 Star (DE3) (Thermo Fisher) transformed with plasmid pIA763 (encodes E ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21 Star strain (Thermo Fisher Scientific). Cell culture was grown to OD600∼0.6 in LB broth containing 100 µg·mL−1 carbenicillin at 37°C and 250 rpm shaking ...
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bioRxiv - Microbiology 2022Quote: ... The presence of recombinant virus was verified by immunostaining with a monoclonal antibody against the RABV nucleoprotein (FujiRebio, Cat# 800–092) and polyclonal antiserum against the SARS CoV/CoV-2 S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate Vero cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Microbiology 2021Quote: ... The presence of recombinant virus was verified by immunostaining with monoclonal antibody against the nucleoprotein (FujiRebio, Cat# 800-092) and polyclonal antiserum against the S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate VERO cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Microbiology 2019Quote: ... with TaqMan fast virus 1-step master mix (Thermo scientific) using AriaMx instrumentation (Agilent).