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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Mouse anti Enterovirus 71 VP1 3656 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: VP1 sequences were PCR amplified from kidney DNA and cloned using the TOPO TA cloning kit (Thermofisher). Clones were screened by restriction digest for the presence of a VP1 sequence and sequenced ...
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bioRxiv - Microbiology 2022Quote: ... RAW 264.7 macrophages (TIB-71, ATCC) were cultured in DMEM (Gibco) containing 10 % FCS (Gibco) ...
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bioRxiv - Plant Biology 2022Quote: ... Phospho-Threonine antibody was obtained from Invitrogen (71-8200, rabbit polyclonal), and used as in a previous study (49) ...
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bioRxiv - Microbiology 2022Quote: ... HA was detected using a rabbit monoclonal antibody (71-5500, Invitrogen). Primary antibodies were detected with anti-mouse Alexa-Fluor 488 and anti-rabbit Alexa-Fluor 594 secondary antibodies (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... A total of 36 μg VP1 plasmid DNA was mixed with 1.5 mL of Opti-MEM I (ThermoFisher). 72 μL of Lipofectamine 2000 was diluted in 1.5 mL of Opti-MEM I and incubated for 5 min at room temperature prior to mixing with the diluted plasmid DNA ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Microbiology 2020Quote: ... Standard curves were generated with 10-fold serial dilutions (108 to 100 copies) of the vp1 gene fragment (138-bp) cloned into a TOPO TA pCR2.1 plasmid (Invitrogen). Copy number in each standard was calculated using formula: where ...
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bioRxiv - Microbiology 2021Quote: ... The VP1 region was amplified by RT-PCR and PCR products were cloned using TOPO-TA Cloning Kits (ThermoFisher) (Primers used ...
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bioRxiv - Molecular Biology 2019Quote: ... and aliquots were incubated with Fc Block (ThermoFisher Scientific 14-9161-71) 20 minutes at 4°C ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Aha1 or Sba1 were exposed to α-HA antibody (Invitrogen #71-5500). Both primary antibodies were diluted 1:5,000 in PBST with 0.2% milk and subsequently conjugated with α-rabbit antibody-HRP conjugate (BioRad Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate #1705046 ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated proteins were also visualized by immunoblot and probed with rabbit polyclonal anti-HA SG77 antibody (1:1000 dilution, 71-5500; ThermoFisher Scientific), mouse monoclonal anti-beta actin (1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Rpl22HA-containing ribosomes were immunoprecipitated (“IP”) from the remaining supernatant by gently mixing with 5µl of rabbit anti-HA antibody (#71–5500 Thermo Fisher Sci) and incubating with gentle rocking at 4°C for 2 hours before isolation with 50 µl of protein A/G magnetic beads previously equilibrated in polysome buffer and a magnetic stand ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IgM-HRP and anti-mouse IgA-HRP (ThermoFisher), goat anti-mouse Igκ ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: The genes encoding for VP1 from BKPyV and JCPyV were codon modified for use in insect cells and synthesized by ThermoFisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2024Quote: ... The VP1 coding region followed by an IRES and the AAP coding region was cloned into the pDEST12.2 OriP (Thermo Fisher) plasmid ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Immunology 2020Quote: RAW264.7 cells (ATCC #TIB-71) were maintained in RPMI 1640 medium (Life Technologies), supplemented with 10% FCS and 2 mM L-glutamine (Eurobio) ...
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bioRxiv - Plant Biology 2023Quote: ... or α-phosphothreonine antibody (α-phosphothreonine, Thermo Fisher, Cat # 71-8200, 1:250) and goat anti-rabbit IgG secondary antibody (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then incubated overnight at 4 °C in 1:100 (in antibody diluent) anti-cadherin-11 antibody (CDH-11, rabbit polyclonal, Thermo Fisher, 71-7600). Cells were washed three times with PBS-tween and incubated in the conjugated secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG or anti-mouse IgA conjugated with HRP (Invitrogen) was added ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 (Invitrogen) 1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Por1 (Invitrogen), and rabbit anti-Sec61 (generous gift from Martin Spiess ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Pgk1 (Invitrogen), mouse anti-Por1 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse (Invitrogen 35518) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-GFP (Invitrogen) and anti-HA (Biolegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... AF594-anti-mouse (Invitrogen). Small molecular compounds such as 2-BP (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-V5 (Invitrogen), STAM (ProteinTech) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-CD79a (ThermoFisher); mouse anti-CD4 (AbD Serotec) ...
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bioRxiv - Microbiology 2021Quote: ... Donkey anti-mouse (Invitrogen) and Donkey anti-rat (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-RetP1 (ThermoFisher) (1:100) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Prdx5 (Invitrogen), rabbit anti-hnRNPK (CST) ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse (Thermo Scientific) or by HRP-conjugated secondaries (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... anti-Mouse IgG (Invitrogen) was used as isotype control ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse (A11017; Invitrogen), Alexa Fluor 488 goat ...
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse Alexa488 (Invitrogen) and anti-rabbit CY3 (Dianova)-conjugated secondary antibodies were diluted 1:600 ...
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bioRxiv - Immunology 2020Quote: ... then anti-mouse (Invitrogen) or anti-human (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse IgG2a (ThermoFisher)] diluted 1:1000 for 1hr at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-GAPDH (Invitrogen)] ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-H3K4me3 (Invitrogen). Secondary antibodies were purchased from Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-GFP (Invitrogen) 1/50 ...
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bioRxiv - Developmental Biology 2021Quote: ... the plates were sealed with MicroAmp Optical Adhesive Film (Thermofisher, cat#: 43-119-71) and allowed to incubate at RT in the dark for at least 15min to rehydrate primer ...
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bioRxiv - Microbiology 2022Quote: The murine macrophage cell line RAW264.7 (ATCC TIB-71) was cultivated in DMEM (Gibco) supplemented with 10 % (v/v ...
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bioRxiv - Pathology 2020Quote: ... Guanosine nucleotide dissociation inhibitor (GDI) (1: 10,000 dilution, 71-0300, rabbit polyclonal, Invitrogen, CA) and a secondary HRP-conjugated goat anti-rabbit antibody (1:2000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... then stained with Invitrogen HA Tag Polyclonal Antibody (SG77) (Thermo Fisher Scientific 71-5500) and Purified anti-His Tag antibody (BioLegend 652502 ...