Labshake search
Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: Individual cytokines in supernatants from cell culture experiments were measured using the species-appropriate ELISA Kits mouse IL-6 (Invitrogen™ 88706488), mouse IL-1beta (Invitrogen™ 88701388) ...
-
bioRxiv - Immunology 2024Quote: IFNγ in supernatants from the whole blood RSA was determined by ELISA according to the manufacturer’s instructions (Human IFNγ Uncoated ELISA kit; Invitrogen). 50μl of supernatant was diluted with 50μl of assay diluent for use in the IFNγ ELISA ...
-
bioRxiv - Cell Biology 2020Quote: Intracellular levels of Ca2+ were measured using the Fluo-4AM Direct Calcium Assay Kit (Invitrogen). STC-1 cells were incubated with a 1:1 ratio of opti-MEM media (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... probenecid (1:250) and PowerLoad concentrate (1:100) (Fluo-4 Calcium Imaging Kit, Molecular Probes) in BrainPhys basal media for 30 min (37 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1X concentration OPP reaction buffer (the last two reagents derived from the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen) and at concentrations relative to those recommended in the manufacturer’s instructions) ...
-
bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to 0.45 μm pore size nitrocellulose membrane in 1×NuPage Transfer Buffer (Invitrogen, CA). SERCA proteins were loaded in 4-12% Bis-Tris Gels (Invitrogen ...
-
bioRxiv - Zoology 2022Quote: ... Proteins from the unstained gel were transferred via standard protocols on PVDF membrane 0.45μm pore (Invitrogen/Thermofisher). Following protein transfer ...
-
bioRxiv - Zoology 2022Quote: ... Proteins from the unstained gel were transferred via standard protocols on PVDF membrane 0.45μm pore (Invitrogen/Thermofisher). Following protein transfer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred towards the negative pole onto a 0.45μm pore size nitrocellulose membrane (88018, ThermoFisher) for 4h at 4°C using an acidic transfer buffer consisting of 0.9mM acetic acid ...
-
bioRxiv - Neuroscience 2021Quote: ... Cultured neurons were incubated with 3 μg/mL of the ratiometric calcium indicator Fura-2 AM (Life Technologies) in external recording solution for 30 min at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized in FACS Buffer (DPBS without calcium and magnesium, 4% FBS, and 2 mM EDTA (Gibco)) with 0.5% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Calcium transients were measured from myocytes paced at 1Hz and loaded with 2 μM Fura2-AM (Molecular Probes) for at least 20 min at 37°C (allowed de-esterification period ...
-
bioRxiv - Cancer Biology 2021Quote: Changes in intracellular calcium concentrations were measured using Fluo-4 Direct™ Calcium Assay (ThermoFisher Scientific, UK) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... Calcium transient (CaT) were recorded with cytosolic free calcium probe Fluo-4 AM (Molecular Probes™, F14201) as published before [27 ...
-
bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on an EASY-Spray C18 reversed-phase nano-LC column (Acclaim PepMap RSLC; length - 50 cm; inner diameter - 2 μm; particle size - 2 μm; pore size – 100 Å; Thermo Scientific) at 55 °C and a flow rate of 300 nL/min ...
-
bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
-
bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
-
bioRxiv - Cell Biology 2021Quote: ... Two sgRNA were produced using the MEGAshortscript Kit (AM1354, Ambion) and purified using the MEGAclear Kit (AM1908 ...
-
bioRxiv - Neuroscience 2023Quote: Two tandem mass tag (TMT) kits (16-Plex TMTpro, ThermoFisher) were used for isobaric labeling24 ...
-
bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
-
bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
-
bioRxiv - Microbiology 2023Quote: ... Both reactions provided two products per reaction that were gel purified in a 2% agarose gel using a GeneJet Gel Extraction kit (Thermo Scientific corp.). Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp. ...
-
bioRxiv - Biophysics 2024Quote: ... The channel RNA was synthesized in vitro using mMESSAGE mMACHINE T7 transcription kit (ThermoFisher, Waltham, MA) from the linearized cDNA.
-
bioRxiv - Developmental Biology 2022Quote: ... or 0.2 μm pores (Invitrogen). After blocking with 5% BSA or 4% skim milk in TBST (10 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Microbiology 2022Quote: ... Western blots were performed on the immunoprecipitated protein samples using the mouse-anti-HA tag (2-2.2.14) primary antibody (Invitrogen) (1:1,000 ...
-
bioRxiv - Microbiology 2022Quote: ... Fifty μL of 1:3000 diluted mouse anti-SARS-CoV-2 Spike Protein S1 monoclonal antibody (GT623, Invitrogen) in 1% BSA-PBS blocking buffer was added and incubated at room temperature for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... or 2 μg of Flag Tag antibody (mouse) conjugated with Dynabeads Protein G Magnetic Beads (Invitrogen, 10004D, USA), or anti-HA magnetic Beads (Abmart ...
-
bioRxiv - Neuroscience 2022Quote: ... Serum TNF and IL-1β concentrations were determined by using a rat TNF alpha Uncoated ELISA and a rat IL-1β ELISA Kit (all from Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Cytokine concentrations were measured by ELISA or Luminex Multiplex Assay (Invitrogen Mouse 20-plex). To determine host susceptibility to endotoxemia ...
-
bioRxiv - Biochemistry 2023Quote: α-synuclein was quantified in the striatum region of mouse brain utilizing the Enzyme-linked Immunosorbent Assay (ELISA) Kit from Invitrogen (Cat No. KHB0061) following the manufacturer‘s instructions.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
-
bioRxiv - Microbiology 2019Quote: ... 2 mL pore water aliquots were fixed with 0.5 mL of 5% (w/w) ZnCl2 (Fisher Scientific). Total dissolved sulfide ...
-
P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: Changes in intracellular calcium response were measured using Fluo-4 Direct™ Calcium Assay (ThermoFisher Scientific, Paisley, UK) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primary myoblasts were transfected at around 30-40% confluence with the Calcium Phosphate transfection kit (Invitrogen). To control the effect of CXCL12 supernatant collected from infected chicken fibroblasts in vitro ...
-
bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
-
bioRxiv - Cell Biology 2019Quote: ... the phase contrast channel was aligned to the fluorescence channel from images of 500 nm fluorescent beads (Invitrogen TetraSpeck) using a custom-written MATLAB program ...
-
bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
-
bioRxiv - Genetics 2021Quote: ... We quantified expression using the IgG4 Human ELISA kit (Invitrogen BMS2095) with IgG4 standards provided in the kit ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... IL-1β and TNFα were analyzed using ELISA kits (Thermo Scientific) following the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: Murine TNF-α and IL-1β were quantified in cell supernatants using ELISA™ Kit from Invitrogen and human IL-1β using Instant ELISA™ Kit from Invitrogen, following manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: The IL-33 Quantikine ELISA kit was purchased from Applied Biosystems and manufacturer instructions were followed ...
-
bioRxiv - Immunology 2020Quote: IL-13 was measured using an eBioscience ELISA kit (Thermo Scientific) using the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Corticosterone levels were determined using the Corticosterone Competitive ELISA kit (Invitrogen) and normalized to the weights of colon pieces measured before the assay.
-
bioRxiv - Synthetic Biology 2020Quote: ... and IFN-β were detected using an ELISA kit (Thermo Scientific) as previously described (26) ...
-
bioRxiv - Bioengineering 2020Quote: ... An IgG4 Human ELISA Kit (Cat # BMS2095, Thermo Fisher, Waltham, MA) was purchased to verify the antibody secretion titer according to the manufacturers instructions.