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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Mouse SETX shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were transfected using Opti-MEM (Gibco, #31985070) and TransIT-X2 (Mirus ...
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bioRxiv - Plant Biology 2023Quote: ... donor plasmid by incubation with BP clonase (Invitrogen) for 1 hour at 25°C ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Plasmids were mixed in warmed Opti-MEM (Gibco) with linear polyethyleneimine (PEI 25kDa ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were transfected into Expi293 cells (Thermo Scientific) according to the manufacturer’s protocol and the protein expressed for 7 days at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... A TEV protease cut site preceding the 6x histidine tag was previously introduced using the Q5 Site-Directed Mutagenesis Kit from NEB and the GeneJET Plasmid Miniprep Kit from ThermoFisher. DAP5 was expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells from Agilent and purified as previously described (Haizel et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli using GeneJet plasmid miniprep kit (Thermo Scientific) and sequences of all plasmids were confirmed using Sanger sequencing performed by Eurofins Genomics.
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bioRxiv - Biochemistry 2023Quote: ... GeneJET plasmid-miniprep-kit (Thermo Fisher Scientific GmbH) was used for plasmid preparations from E ...
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bioRxiv - Molecular Biology 2023Quote: ... Assembled plasmids were transformed into Mach1 (Invitrogen #C862003) or STBL3 (from Q3 Macrolab at University of California ...
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bioRxiv - Genetics 2023Quote: ... into gateway-compatible pENTR-D/TOPO plasmid (Invitrogen). We then cloned 482 bp of the AtHTA11 terminator sequence (482 bp downstream from the AtHTA11 stop codon ...
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bioRxiv - Microbiology 2023Quote: ... and L plasmids with Lipofectamine 2000 (Thermofisher, 11668019). Cells were incubated for 24 hours until imaged for mCherry Expression or processed for qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected using Neon transfection system (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng plasmid DNA and lipofectamine 3000 (Invitrogen) as indicated ...
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bioRxiv - Biochemistry 2023Quote: The plasmid pBAD/Myc-His A (pBAD) (Invitrogen) and pET28a (+ ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into BL21(DE3) (Invitrogen) and 35 ml starter culture with 2xTY containing 100 µg/ml carbenicillin (Goldbio ...
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bioRxiv - Synthetic Biology 2024Quote: ... or a PureLink Quick Plasmid Miniprep Kit (Invitrogen). We performed Sanger sequencing on multiple stocks of the BFP control plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmids were transfected into Expi293F cells (ThermoFisher) using ExpiFectamine 293 Tranfection Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... purified with PureLink HiPure Plasmid Midiprep Kit (Thermofisher). After transfections ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were performed in OPTI-MEM (Invitrogen) with 2 μl Lipofectamine 2000 per well for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid transfections were performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Control pCDNA3.1 empty plasmid was obtained from Invitrogen. Rabbit polyclonal anti-human C12orf23 (TMEM263 ...
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bioRxiv - Bioengineering 2024Quote: ... The pcDNA™3.1(+) plasmid (ThermoFisher, Waltham, MA) and the synthesized HLA-E gene were digested separately using HindIII and EcoRI enzymes (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG or anti-mouse IgA conjugated with HRP (Invitrogen) was added ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min and rinsed three times with TBS-T (0.05 % tween-20) ...
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bioRxiv - Cancer Biology 2021Quote: ... which were then transfected with a combination of PG13-luc assay plasmid and a control plasmid pRL-SV40P (50:1) using Lipofectamine 2000 (Invitrogen, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Cell Biology 2021Quote: ... hTERT-RPE1 cells were transfected with donor plasmid and AIO plasmid with the appropriate gRNAs using a Neon transfection system (Thermofisher Scientific). For 5 × 106 cells ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day PcDNA 3.1/ Twist1-BirA*-HA plasmid or PcDNA 3.1/ GFP-BirA*-HA plasmid was transfected into cells using Lipofectamine® 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RPE-1 wildtype cells were transfected with the CRISPR/Cas9 plasmid containing the PCNA gRNA and the donor plasmid containing the K164R mutation using the Neon Transfection System (Invitrogen MPK5000) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... were transformed with the modified transfer vectors and before plasmid DNA extraction using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μg of reporter plasmid and 15 ng of Cre-plasmid was mixed with 3 μL Lipofectamine LTX reagent (Invitrogen, #15338100), 1.5 μL PLUS reagent and 100 μL Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... This plasmid consists of six inserts and the plasmid cloning vector CloneJet pJET1.2/blunt (2974 bp; Thermo Fisher Scientific, Massachusetts, USA). For the inserts ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted using the GeneJET Genomic DNA Purification Kit while plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... were used for sequencing the full length of cloned PCR product after plasmid purification using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen, V35120) in frame and linked to mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... Transient overexpression of VDR in SH-SY5Y cells was performed by transfecting a VDR expression plasmid (pcDNA3-VDR) or a mock control plasmid (pcDNA3) with Lipofectamine 2000 (Invitrogen, USA). For mammalian two-hybrid assays to assess the interaction between VDR and RXR ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Molecular Biology 2020Quote: ... K562#17 cells were transfected with 32 μg of barcoded IPR-PB-BC plasmid library and 6 μg of PB transposase plasmid using Lipofectamine 2000 (ThermoFisher #11668019). Mock-transfected (without PB transposase ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were extracted with NucleoSpin Plasmid Transfection-grade (Machery-Nagel) and concentration were measured on Nanodrop ND-1000 (Thermo Fisher Scientific). All plasmids were sequenced by Sanger method (Giga-Genomics).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNAs were cloned into pDONR221 plasmid and introduced into the PB-Hyg-Dest plasmid using Gateway technology (Thermo Fisher Scientific). The PB-Hyg-Dest-mCherry-PAR-6B or the PB-Hyg-Dest-LifeAct-Ruby plasmid was co-transfected with the piggyBac plasmid using Lipofectamine 3000 to generate Hygromycin B resistant stable cell lines ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA from the overnight cultures was harvested via extraction with the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific), and insert sequence was confirmed by Sanger sequencing at the on-site DNA Sequencing Facility (Dept ...
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bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... target plasmid was transfected together with psPAX2 and pMD2.G packaging plasmids into 293T cells using lipofectamine 2000 transfection reagent (Invitrogen, 11668027). Media was changed 24h later and lentivirus supernatant was collected after an additional 24 h ...