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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Mouse MOB1B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and sgRNA or shRNA using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
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bioRxiv - Neuroscience 2020Quote: ... cultured hippocampal neurons treated with adenoviruses expressing either βII-spectrin shRNA or scrambled (control) shRNA were lysed on ice using the RIPA buffer (Thermo Fisher Scientific, 89900) complemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... shRNA was synthesized using the T7 MegaShortScript kit (Invitrogen, AM1354) with an incubation time of 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... shRNA/siRNA transfection was carried out with Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... The corresponding direct and reverse oligos for every shRNA (Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Non-silencing shCTRL = RHS4743) lentiviral shRNAs were purchased from ThermoFisher Scientific (Thermo ...
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bioRxiv - Immunology 2020Quote: ... and sgRNA or shRNA lentiviral construct using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
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bioRxiv - Neuroscience 2022Quote: ... and scrambled shRNAs were transfected into the cells using Lipofectamine (GIBCO). Seventy-two hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... and NCBP1 were selected from the GIPZ Lentiviral shRNA library (ThermoFisher, sh-eIF4E2 ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral shRNA constructs were obtained from Open Biosystem (Thermo Fisher Scientific). Targeting sequences were cut from the pGIPZ constructs using MIuI and XhoI sites and cloned into pInducer10 using the same sites ...
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bioRxiv - Developmental Biology 2022Quote: ... Concentrations of shRNA was measured with a NanoDrop One (Thermo fisher).
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bioRxiv - Developmental Biology 2023Quote: ... HaCaT SAM and shRNA cells were done using Lipofectamine 2000 (Invitrogen). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Certified scramble shRNA (ACTACCGTTGTTATAGGTG) was also used as a negative control (Invitrogen). All plasmids used in this study were purified by NucleoBond Xtra purification kit (MACHEREY-NAGEL) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells with integrated shRNA were selected with 300μg/ml Hygromycin B (Invitrogen). The GFP expressing cells were isolated by FACS sorting (BD FACSAria Fusion).
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bioRxiv - Molecular Biology 2022Quote: ... Patz1 shRNA sequences were designed using BLOCK-iT RNAi designer tool (Invitrogen). Sense and antisense oligos were annealed and cloned into pENTR/U6 plasmid (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and the Ido1 shRNA using Lipofectamine 2000 (ThermoFisher Scientific, Cat. No. 11668027). Cells were transfected in virus collection medium (DMEM supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while pLL3.7-shRNA infected cells were selected with 2μg/ml puromycin (Gibco). The overexpression and knockdown efficiencies were validated with WB and qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: The seed shRNA sequences were designed by BLOCK-iT RNAi Designer (ThermoFisher) and cloned into lentiviral expressing vector pTY-U6-Pgk-Puro ...
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bioRxiv - Microbiology 2020Quote: Individual shRNAs were designed using the BLOC-iTTM RNAi Designer (Thermo Fisher Scientific). The specificity was tested by BLAST (NCBI) ...
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bioRxiv - Molecular Biology 2021Quote: ... the expression of shRNA was induced by addition of doxycycline (Thermo Fisher Scientific). To this end ...
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bioRxiv - Molecular Biology 2019Quote: ... These shRNA sequences were generated using an open-access algorithm (RNAi designer, ThermoFisher) and were as follows ...
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bioRxiv - Biochemistry 2020Quote: ... Adenoviruses expressing these shRNAs were established as per the manufacturer’s instructions (Life Technologies) and as previously reported 50 ...
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bioRxiv - Neuroscience 2021Quote: ... shTTL2 and control shRNA (packaging vectors pLP1, pLP2, and pLP-VSV-G, Thermofisher) were produced by co-transfection with the psPAX2 and pCMV-VSV-G helper plasmids ...
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bioRxiv - Cell Biology 2022Quote: K8 expression was silenced in HeLa cells using a stable shRNA (Thermo Scientific) transduced by lentiviral vectors ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were transfected with shRNA constructs using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and 48 h post-transfection selected with 4 µg/ml puromycin (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Lentivirus expressing shRNAs were produced by DNA transfection using Lipofectamine 3000 (Thermo Fisher). Transduction was performed using Polybrene (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Stable shRNA-expressing cell lines were maintained in 2.5 μg/ml puromycin (Gibco) following initial selection at 5 μg/ml.
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bioRxiv - Neuroscience 2021Quote: ... the mouse ephrin-B3 was cloned into pSecTag-B plasmid (Termo Fisher Scientific, Waltham, USA). Six repeats of the myc epitope were cloned at the carboxyl end of ephrin-B3 ...
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bioRxiv - Cell Biology 2020Quote: ... Targeting sequence of SEPT9 shRNA was designed using BLOCK-iT™ RNAi Designer (Thermofisher) and the sequence was scrambled using the Invivogen shRNA wizard.
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bioRxiv - Developmental Biology 2022Quote: ... and FOXA2 shRNA knockdowns VFG culture was fixed in 1% formaldehyde (Thermo Scientific, 28906) in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit ...
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bioRxiv - Neuroscience 2021Quote: ... A control vector with scrambled non-silencing shRNA 59 was obtained from Thermo Scientific, and the shRNA to knock-down plasmid for mouse eIF4EBP1 was obtained from Sigma Aldrich (TRCN0000335381).
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bioRxiv - Microbiology 2023Quote: ... and 100ng of the TMER or shRNA vector was transfected with Lipofectamine 2000 (Thermofisher). After 48 hours the efficiency was checked visually by GFP control plasmid transfection ...
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bioRxiv - Genomics 2024Quote: ... and from SW480 cells stably expressing Ctrl or TOP1 shRNAs using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Five shRNAs (Table S1) targeting to porcine Bmp15 mRNA was designed and selected by Invitrogen’s web-based siRNA design software (https://rnaidesigner.invitrogen.com/rnaiexpress/) ...
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bioRxiv - Molecular Biology 2019Quote: Short hairpin RNA (shRNA) sequences specifically targeting circE7 back-splice junction were designed from Invitrogen BLOCK-ItTM RNAi Designer (https://rnaidesigner.thermofisher.com/rnaiexpress/) ...
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bioRxiv - Molecular Biology 2019Quote: ... Media for HeLa1.2.11 and HCT116 shRNA knockdown cells also contained puromycin (1 μg/mL, Gibco) to maintain selection (Stewart et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The corresponding direct and reverse oligos for every shRNA (Invitrogen; Thermo Fisher Scientific, Waltham, USA) were annealed and subcloned on a MLP plasmid vector which contains a green fluorescent protein sequence (GFP ...
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bioRxiv - Neuroscience 2021Quote: ... The shRNA sequence was selected using BLOCK-iT™ RNAi Designer provided by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: NHE6-GFP in FuGW and shRNA constructs were transfected using lipofectamine 3000 (Invitrogen, Carlsbad, CA) into HEK293-FT cells to be packaged into virus using pCMV-Δ8.9 and PMDG at a ratio of 9:8:1 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg of corresponding shRNA vectors were transfected into the cells using Lipofectamine 3000 (Invitrogen). Cells were harvested 48 hours after transfection for mRNA extraction using the RNAeasy mini plus kit (Qiagen) ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Cancer Biology 2024Quote: ... Selection for shRNA transduced cells was performed using 2 µg/ml Puromycin DiHCL (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 μg each of plasmid were transiently transfected into E14tg2A mouse stem cells using Lipofectamine 2000 (ThermoFisher) in suspension according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Stable transfections of NIH – vGPCR for different shRNAs were performed using the Lipofectamine Plus Reagent (Invitrogen). NIH-vGPCR cells were plated at 60% confluence in 10 cm plates and transfected with 2ug of shRNA (shScramble and shRNAs targeted against Nrf2 - GIPZ Nfe2l2 shRNA Thermo RMM4532-EG18024) ...
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bioRxiv - Biochemistry 2021Quote: ... adenoviruses expressing LacZ and Tpi shRNA were cloned into the Adenoviral Gateway Expression System (Invitrogen, CA). In brief ...
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bioRxiv - Genomics 2021Quote: ... The WTC11 iPSCs transduced with shRNA lentivirus were treated with hygromycin (Gibco, 10687010, 150 µg/mL) for 7 days and then collected for RNA extraction and qPCR.
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bioRxiv - Evolutionary Biology 2022Quote: ... and shRNA for TP53 into 300,000 fibroblasts from each individual with a Neon Electroporation Device (Invitrogen), using a 100 μL kit ...
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bioRxiv - Cell Biology 2023Quote: ... Stable clones expressing the specific shRNAs were selected using 5μg/ml of puromycin (Gibco-life technologies) and pooled to generate the cells used in the experiments.
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bioRxiv - Cell Biology 2023Quote: ... Selection of stably shRNA-expressing polyclonal cell lines was achieved using puromycin (1 µg/mL; Gibco). pLKO.1 with non-targeting sequence 5’-AATTGCCAGCTGGTTCCATCA-3’ was used to generate shRNA control (shCON ...