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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Mouse Fibroblast Growth Factor 15 FGF15 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... AEC2 and mesenchymal cells were co-cultured (5,000 AEC2:30,000 mesenchymal cells/well) in a modified MTEC media diluted 1:1 in growth factor-reduced matrigel (Cat#CB-40230A; Thermo Fisher). Modified MTEC culture media is comprised of small airway basal media (SABM ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown as monolayers on geltrex-coated surfaces (Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, Gibco, A1413201) in NPC expansion medium ...
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bioRxiv - Developmental Biology 2019Quote: ... Medium for H9 and PEN8E-derived organoids was supplemented with 20 ng/ml insulin-like growth factor 1 (IGF1; ThermoFisher Scientific) and 55 nM beta-mercaptoethanol (2-ME ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... were purchased from EBISC (European Bank for Induced pluripotent Stem Cells) and maintained on Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) in Essential 8™ Flex Media Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Five million C4-2 or two million SK-OV-3 cells in serum free media were mixed one to one with growth factor reduced Matrigel® (Invitrogen) in a total volume of 200 μl and injected in the hind flank using a 23- or 27-gauge needle ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... They were cultured using Keratinocyte-SFM medium with 50 µg/mL bovine pituitary extract and 5 ng/mL epidermal growth factor (ThermoFisher Scientific) in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then seeded in laminin-coated tissue culture plates and cultured in the presence of 50 ng/μL nerve growth factor (NGF) (Thermo Fisher) unless otherwise noted.
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bioRxiv - Developmental Biology 2021Quote: ... Pancreatic progenitors differentiated from hESCs at the end of Stage 4 using the described pancreatic differentiation protocol were dissociated into single cells with TrypLE and resuspended in a 3:1 mixture of growth-factor reduced Matrigel and expansion medium (DMEM/F12 Glutamax (Thermo Fisher), 10 μM ROCK inhibitor Y-27632 ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... an additional 100 μL of EGM-2 without growth factor was added with 1.5 μL of 1 mg/mL calcein-am (ThermoFisher Cat # C1430). Cells were then imaged 30 minutes later at the 4-hour timepoint ...
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bioRxiv - Cancer Biology 2022Quote: Organoid growth was evaluated in growth factor–free (GF-free) medium (Advanced DMEM/F12 [Gibco, Thermo Fisher Scientific, Waltham, MA, USA] supplemented with 1x GlutaMax [Gibco]) or StemPro medium (StemPro hESC [Invitrogen] ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and 62.5 ng of cDNA was amplified using TaqMan probes for vascular endothelial growth factor alpha (Vegfa) and 18S as endogenous control (Life Technologies), and SYBR green probes for cd93 ...
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bioRxiv - Cell Biology 2023Quote: ... 30,000 fibroblasts/mesenchymal cells per well) in modified MTEC medium diluted 1:1 in growth factor-reduced Matrigel (cat. no. CB-40230A, Thermo Fisher). Modified MTEC culture medium is composed of SABM with insulin ...
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... spheres were plated as neural progenitors onto a 10cm dish precoated with growth factor reduced (GFR) Matrigel (CB-40230A, Fisher Scientific). Neural progenitors were allowed to form neuronal rosettes and sustained in N2B27 media alone for days 8–15 ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were grown also on plates coated Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Cancer Biology 2024Quote: ... 150,000 KPARG12C or 400,000 3LL-ΛNRAS cells were mixed 1:1 with Geltrex LDEV-Free Reduced Growth Factor matrix (Thermo Fisher Scientific) and injected subcutaneously into one flank ...
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bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
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bioRxiv - Bioengineering 2023Quote: ... Individual strips with a small amount of medium were then carefully moved to pre-chilled micro-centrifuge tubes and then mixed with cold reduced growth factor basement membrane matrix Geltrex (Thermo Fisher). The dilution of Geltrex by the introduced medium was less than 20% ...
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bioRxiv - Cancer Biology 2024Quote: ... with 50,000 mouse endothelial cells (harvested from mouse lungs by CD31 selection and expanded in vitro as previously described 67) and resuspended in 50 µl of Geltrex™ reduced growth factor basement membrane matrix (A1413301, Gibco). 100 µl of 1:1 GFP+:endothelial cell mixture was plated on transwell inserts with 0.4 µm pores and allowed to solidify for 30 mins in a humidified CO2 incubator (EOE ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 mouse embryos and grown in ‘MEF medium’ containing DMEM (41966029, Thermo Fisher Scientific) supplemented with 10% FBS (10270106 ...
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bioRxiv - Immunology 2020Quote: Transcription factor (TF) staining was carried out after cells were fixed using the eBioscience FoxP3 transcription factor staining kit (ThermoFisher) or BD Cytofix (BD Biosciences ...
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bioRxiv - Genetics 2019Quote: ... Cells were maintained and propagated on mouse embryonic fibroblasts (MEFs) in hESC media [DMEM/F12 (Gibco) with 10% KSR (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) at room temperature.
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bioRxiv - Microbiology 2020Quote: ... Mouse fibroblast L929 cells (ATCC CCL-1) were maintained in DMEM medium-Glutamax (10566-016; Gibco) supplemented with 10% FBS and antibiotics.
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bioRxiv - Cancer Biology 2022Quote: ... NMR and mouse SV40-Ras skin fibroblasts were cultured in DMEM high glucose (#41965-039; Gibco) supplemented with 15% fetal bovine serum (#F7524-500ML ...
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bioRxiv - Microbiology 2023Quote: ... C57Bl/6J mouse embryonic fibroblasts (B6 MEFs) and RAW 264.7 macrophages were maintained in DMEM (GIBCO) supplemented with 2 mM glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... we transfected each AAV.Nme2Cas9.sgSfrp2 into immortalized striped mouse immortalized fibroblasts using lipofectamine3000 (L300008, Thermo Fisher). ∼3-4 days post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse embryonic fibroblasts (MEFs) were cultured in high glucose and phenol red-free DMEM (21063045, Gibco) with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Cell Biology 2024Quote: HT29 cells and mouse dermal fibroblasts were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies) with 8% v/v fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse or naked mole-rat fibroblasts were incubated with 10 µm Celltracker fluorescent probes (C34565, Invitrogen) for 45 minutes at respective growth conditions and then plated together with SCC25-Luc-GFP in ultra-low attachment plates using SCC25-Luc-GFP culture conditions ...
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bioRxiv - Genomics 2024Quote: ... Mouse fibroblast cells (NIH-3T3) were cultured in DMEM medium (high glucose, Gibco, catalog no. 11965092) supplemented with 10 % FBS ...
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bioRxiv - Genetics 2022Quote: ... fibroblasts were routinely cultured in IMDM/Glutamax containing 15% fetal bovine serum (FBS) and 1% penicillin/streptomycin (all Invitrogen, Waltham, MA). Normal Primary Human Dermal Fibroblasts (HDFa ...
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bioRxiv - Neuroscience 2020Quote: ... human fibroblasts were cultured in fibroblast culture medium [DMEM high glucose (Life Technologies) plus 10% FBS (Life Technologies)] ...
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bioRxiv - Cell Biology 2020Quote: WT cardiac fibroblasts were nucleofected with pCDNA3.1 tdTomato (Neon Transfection kit, Invitrogen, MPIK10025), diluted 1 in 2 with control cells (cell nucleofected with no DNA) ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were isolated from fibroblasts using the PureLink RNA Mini Kit (Invitrogen). The reverse transcription and quantitative PCR reactions were performed as detailed previously (Garre et al ...
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bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
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bioRxiv - Neuroscience 2024Quote: ... was reprogrammed from fibroblasts using the CytoTune 1 non-integrating kit (Thermo Fisher). iPSC media was changed every day ...
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bioRxiv - Bioengineering 2022Quote: ... mouse brains were submerged in 15% sucrose (Thermo Fisher Scientific) in 1X PBS for 6-12 hours and then in 30% sucrose in 1X PBS for 24 hours ...
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bioRxiv - Physiology 2024Quote: ... Ly6g rat anti-mouse PE-Cyanine5 (Invitrogen - 15-9668-80), F4/80 anti-mouse APC (eBioscience 17-4801-82) ...
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bioRxiv - Cancer Biology 2023Quote: ... The Foxp3 Transcription Factor Staining Buffer kit (Invitrogen, 00-5523-00) was used following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Mitogenic stimulus conditions were applied immediately after irradiation and consisted of control medium with the addition of 1 ng/mL TGF-β1 and 5 ng/mL basic fibroblastic growth factor (bFGF) (PHG9204 and PHG0264; Thermo Fisher Scientific). In additional experiments with human cartilage ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were re-suspended in ECGM containing 10% FBS and 40 ng/ml basic fibroblastic growth factor (bFGF; Thermo Fisher Scientific-Invitrogen, UK) and seeded at 5 × 104 cells/well (n=6) ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...