Labshake search
Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Mouse Fc Receptor Like Protein 1 FCRL1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... 1 μL pre-washed magnetic-conjugated protein G beads (Dynabeads protein G, Invitrogen) were added to the reactions followed by the incubation for 1 hour at 4°C in the thermomixer (750 rpm) ...
-
bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
-
bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: We used the following mouse monoclonal antibodies against: transferin receptor (13-6800, Invitrogen, 1/200); CD63 [53] ...
-
bioRxiv - Physiology 2023Quote: ... The anti-transferrin receptor mouse monoclonal antibody (TransR, clone H68.4, Thermo Fisher Scientific, 1:1000) and the anti-alpha 1 Na+/K+-ATPase mouse monoclonal antibody (Na+/K+-ATPase α1 ...
-
bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
-
bioRxiv - Immunology 2023Quote: ... Clec12a-Fc or Fc only fusion proteins were isolated with Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher Scientific, Catalog number: 88804), quantified with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific Catalog number ...
-
bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
-
bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
-
Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... or Protein G (Thermo Fisher Scientific, Cat. #10002D, for mouse). HEK293T lysates were supplemented with 1.5 mM CaCl2 and 1 μM calmodulin (final concentrations ...
-
bioRxiv - Systems Biology 2021Quote: ... immunoprecipitated proteins were detected with monoclonal mouse anti-V5 (Invitrogen) or mouse anti-HA.11 (BioLegend ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding mouse PRMT proteins were subcloned into pFASTBAC1 (Invitrogen) with a FLAG tag ...
-
bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and Transferrin Receptor (1:1,000; Invitrogen). Proteins were detected with HRP-conjugated secondary antibodies (1:10,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... anti‐transferrin receptor (1:500; Invitrogen), anti‐α‐fodrin (1:5000 ...
-
bioRxiv - Immunology 2019Quote: ... Protein A and Protein G coupled magnetic beads (mixture 1:1, Dynabeads, Thermo Fisher, 10002D and 10004D) were pretreated with BSA (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
-
Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
-
bioRxiv - Bioengineering 2022Quote: ... A 40 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for an additional 4 h at 4°C on a rotator (400 rpm) ...
-
bioRxiv - Immunology 2023Quote: ... A 20 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4 h at 4°C with rotation (50 rpm) ...
-
bioRxiv - Cell Biology 2019Quote: ... Human insulin-like growth factor 1 (IGF-1) was from Thermo Fisher and used as IGF-1 receptor activator.
-
bioRxiv - Biochemistry 2019Quote: The protein sample (∼1 μg) was mixed with Protein Thermal Shift Dye (Applied Biosystems) and 10∼10,000 μM concentration of each amino acid in 20 μL of assay buffer and loaded to a MicroAmpR Fast Optical 48-Well Reaction Plate (Applied Biosystems) ...
-
bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100 µL FACS buffer supplemented with Fc-γ receptor anti-CD16/CD32 antibodies (1:100, Thermo Fisher Scientific) and incubated on ice at 4°C for 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... ISG MX dynamin-like GTPase 1 (Mx1, Thermo Fisher, Mm00487796_m1) expression was assessed in select samples ...
-
bioRxiv - Genomics 2024Quote: ... and NRG1 EGF-like domain (ThermoFisher; MA4-12896, 1:100). Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Mouse anti-human transferrin receptor antibody was from Life Technologies. Secondary IRDye-labeled goat anti-mouse and anti-rabbit IgG antibodies were from LI-COR Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... and allophycocyanin-conjugated mouse anti-glucocorticoid receptor (GR-APC; Invitrogen; cat# 17-6189-82 ...
-
bioRxiv - Biochemistry 2024Quote: Fc fusion proteins were expressed in ExpiCHO cells using the ExpiCHO expression system kit (Thermo Scientific, A29133) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
-
bioRxiv - Cell Biology 2022Quote: ... medium was replaced to low serum RPMI (RPMI-1640 supplemented with 1% HS and 1% FBS) with 50 ng/ml NGF 2.5S Native Mouse Protein (Gibco) to differentiate cells for desired time ...
-
bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
-
bioRxiv - Developmental Biology 2022Quote: ... and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... the cells were pre-treated with Fc receptor binding inhibitor polyclonal antibody (eBioscience™, Thermo Fisher Scientific) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein extracts were then incubated for 1 hour with protein A-conjugated Dynabeads (Life Technologies), after which the beads were washed with lysis buffer at incremental KOAc concentrations (100 mM ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher). The remaining 9 µl were mixed with SDS (0.2% final) ...
-
bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
-
bioRxiv - Cell Biology 2022Quote: ... HRP conjugated secondary antibodies against mouse proteins were obtained from ThermoFisher and used at 1:5000 dilution.
-
bioRxiv - Physiology 2021Quote: ... mouse-anti Microtubule-associated protein 2 (MAP2) (Invitrogen, Cat # 13-1500) (1:1000) ...
-
bioRxiv - Physiology 2021Quote: Mouse-anti Paired box protein Pax-6 (PAX6) (Invitrogen, Cat #21103049) (1:300 ...
-
bioRxiv - Immunology 2020Quote: ... Supernatants containing protein were analyzed by Mouse Multiplex Luminex assay (Invitrogen) at the Human Immunology Core at University of Pennsylvania ...
-
bioRxiv - Neuroscience 2022Quote: ... solubilized hippocampal mouse brain and plasma proteins were quantified (Thermo Fisher EZQ Protein Quantitation Kit or the Pierce BCA) ...
-
bioRxiv - Microbiology 2023Quote: ... or mouse IgG CTRL coupled to Dynabeads protein G (Life Technologies). The beads were washed 4 times with lysis buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
-
bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...