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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... incubation for 45 minutes at 37°C with 1:300 goat anti-mouse AF647 antibody (Life Technologies, A21237) diluted in PBS + 1 % BSA and three more washes in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and Transferrin Receptor (1:1,000; Invitrogen). Proteins were detected with HRP-conjugated secondary antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... anti‐transferrin receptor (1:500; Invitrogen), anti‐α‐fodrin (1:5000 ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Bioengineering 2019Quote: ... samples were incubated with mouse-anti-Pax7 (1:10, DSHB) overnight at 4°C followed by AlexaFluor-647 antibody (1:500, Invitrogen, A31573) for two hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Larvae were rinsed in PBST three times for 20 min and incubated overnight at 4°C with secondary antibodies (anti-mouse 1:500 IgG-Alexa Fluor 488, 1:500 IgG Alexa Fluor 594, Molecular Probes; anti-rabbit IgG-Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... blocked and imaged as described under section triple-color immunostainings but were incubated with mouse anti-AnkG (#AB_10675130, Neuromab, 1:200) overnight at 4°C and subsequently with donkey anti-mouse AF647 (#A31571, Invitrogen, 1:800) for 1h.
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bioRxiv - Immunology 2021Quote: ... then Fc receptors blocked with normal mouse serum (Thermo Fisher) and TruStain FcX αmouse CD16/32 (Biolegend ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse anti-human transferrin receptor antibody was from Life Technologies. Secondary IRDye-labeled goat anti-mouse and anti-rabbit IgG antibodies were from LI-COR Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and allophycocyanin-conjugated mouse anti-glucocorticoid receptor (GR-APC; Invitrogen; cat# 17-6189-82 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cytokines and chemokines secretion were determined by 36-Plex Mouse ProcartaPlex (ThermoFisher, EPX360–26092–901) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... lung homogenates were filter sterilized and then analyzed with Mouse Chemokine/Cytokine Panel 1A (ThermoFisher) on a Luminex xMAP FlexMAP3D (Luminex Corp ...
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bioRxiv - Cell Biology 2020Quote: ... Cytochrome c oxidase subunit 1 (COX1; 459600) from Invitrogen; Cytochrome c oxidase subunit 2 (COX2 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µL of Streptavidin C-1 beads (Thermo Fisher) were washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Systems Biology 2021Quote: ... Frost® Cryo 1°C Freezing Container (ThermoFisher scientific) with Isopropyl alcohol at −80°C over-night ...
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bioRxiv - Microbiology 2022Quote: ... HRP (ThermoFisher; 1:5000, 60 min at 4°C). Detection was achieved with Pierce™ ECL Western Blotting Substrate using a ChemDoc XR+ (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... c-Myc (Invitrogen, clone 9E10, MA1-980, 1:1000), V5 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight at 4° C (MAP2 1:1000 Invitrogen #MA5-12826 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C and total protein determined by the BCA method (Thermo Fisher Scientific). Proteins were separated on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... was carried out overnight at 4°C using Protein A Dynabeads (ThermoFisher Scientific). The mononucleosomal fraction (147 bp ...
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bioRxiv - Bioengineering 2023Quote: ... protein expression was induced overnight at 16°C with 0.2mM IPTG (Thermofisher, 15529019). The overnight cultures were lysed and sonicated in non-denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and protein concentrations were quantified using a CBQCA Quantification kit (C-6667, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted by incubation at 95°C in NuPAGE Loading Buffer (Invitrogen) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed overnight at 18°C in Terrific Broth (Thermo Scientific) and 0.4% v/v glycerol with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitations were performed overnight at 4°C with Dynabeads Protein G beads (Invitrogen) conjugated to mouse anti-FLAG M2 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... thermoblocks at 90°C and 50°C (e.g., Thermo Fisher, 88870004), an ultracentrifuge capable of spinning 50 ml falcon tubes at 10,000 rpm (Beckman Coulter Avanti J-30 I ultracentrifuge and a Beckman JA-12 fixed angle rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... Fast SYBR™C Green Master Mix (Applied Biosystems™C) was employed along with primers at a concentration of 5nM ...
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bioRxiv - Microbiology 2021Quote: ... followed by 40 cycles at 95 °C (15 s) alternating with 60 °C (1 min) (Applied Biosystems, Carlsbad, CA). Gene-specific products were normalized to a group of 5 housekeeping genes (ACTB ...
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bioRxiv - Microbiology 2021Quote: ... and 1 min at 60 °C cycle of 50 °C with a 7500 Real-Time PCR System (Applied Biosystems). The following Taqman primer and internal probes were used:
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and incubated overnight at 4°C with rat anti-mouse CD8 (4SM15, Invitrogen) and rabbit anti-mouse Ly6G (E6Z1T ...
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bioRxiv - Molecular Biology 2021Quote: ... and a layer of mitomycin C-treated mouse embryonic fibroblasts (MEFs) (Thermofisher Scientific).
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bioRxiv - Biochemistry 2022Quote: Commercially available primary antibodies used: mouse anti-c-myc (Invitrogen, dilution immunoblot (IB) 1:2’000 ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein concentration was determined by BCA assay and 500 μg of total proteins was incubated for 1 hour (4°C, rotary agitation) with 25 μl of Pierce™ Protein A Agarose beads (ThermoFisher, cat. no. 20333), centrifuged at 2500 g for 3 minutes at 4°C and the supernatant recovered as pre-cleared lysate ...
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bioRxiv - Cancer Biology 2023Quote: ... and patient-derived cell cultures (P022.C, P024.C, P030.C) were cultured in Dulbecco’s modified Eagle medium (DMEM, #41966, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...