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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plasmids were extracted using the PureLink Quick Plasmid Miniprep kit (Invitrogen) before transfer to M ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified using a GeneJet Plasmid Miniprep kit (Thermo Scientific). 50µg/mL Phleomycin (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid was purified (Purelink HiPure Plasmid Maxiprep Kit, Invitrogen, CA) from transformed Stellar Competent Cells (Takara Bio Inc ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were purified using the Genjet Plasmid Miniprep MKit (Thermo Scientific). LPC (15:0 ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmids was recovered with GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and the DNA was sequenced with M13 common sequencing primers ...
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bioRxiv - Physiology 2020Quote: ... Plasmids were isolated using PureLink HiPure Plasmid Midiprep Kit (Thermo Scientific) and sequenced by GATC Biotech AB (Köln ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Physiology 2021Quote: ... Plasmid DNA was isolated (PureLink Quick Plasmid Miniprep kit; Invitrogen K210010) according to manufacturer specifications and inserts were sequenced to confirm their identity and uploaded to NCBI (MW962257).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The plasmid was purified (Purelink HiPure Plasmid Maxiprep Kit, Invitrogen, CA) from transformed Stellar Competent Cells (Takara Bio Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... sgRNA plasmids and donor plasmids were transfected with Lipo3000 Reagents (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: Primary neurons expressing lentivirally delivered clathrin heavy chain (CHC)-targeting shRNA or a scramble version (DIV14) were starved for 1 hour in osmolarity-adjusted NBA medium (Gibco) at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs and Gapmers or shRNAs were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... Validation of shRNA constructs for reduction of Arl8b was done by transfecting mouse N2a cells at 40% confluency with pLKO3.G-Arl8b shRNA #1 and #2 either separately or together using Lipofectamine 2000 (Invitrogen). Transfection rates were > 80% and were ascertained by counts of GFP fluorescence on cell bodies ...
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bioRxiv - Immunology 2019Quote: ... expressing shRNA targeting human CACNA1F gene or pGFP-V-RS vector with scrambled shRNA cassette (control) was transfected into Jurkat T cells by Neon electroporation system (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... pINDUCER10 expressing shRNAs (Table S1) was transiently transfected in Akata-BX1 cells using TurboFect™ transfection reagent (Thermo fisher scientific) according to the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... each LeGo plasmid was transfected into HEK293T cells together with the Trans-Lentiviral shRNA Packaging System (#TLP5912; Dharmacon) helped by Lipofectamine 2000 (#11668030; Invitrogen). Forty-eight hours post-transfection viral particles in the supernatant were collected ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from HNs after 8 days of tough decoy and shRNA treatment using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... NCH644 cells grown on laminin-coated plated were infected at an MOI of 0.3 with a 2.6 K custom lentiviral shRNA library constructed in pGIPZ puro vector and targeting 574 DNA Damage Response (DDR) genes (i.e., ∼4.5 shRNAs per gene on average, 700-fold representation)(Thermo Fisher Scientific). Cells were exposed to 1 μg/ml puromycin for 96 h to allow selection of transductants ...
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bioRxiv - Developmental Biology 2020Quote: ... Forward and reverse oligos of 66 bases in length corresponding to the DNA templates for in vitro transcription of shRNAs (Supp. Table S1) were ordered from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Subcellular fractionation was performed on the HAP1 PANK1 KO cells stably expressing the shRNAs following the manufacturer’s protocol (ThermoFisher #78840). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were transfected with shRNA clones (as mentioned in the figures and legends) using Lipofectamine LTX with Plus Reagent (Invitrogen) for a maximum of 48hrs before any further analysis.
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transduced with Tet-pLKO-Puro (with shRNAs targeting Cx31 or GFP control) followed by puromycin (2 ug/mL; Gibco) for seven days ...
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bioRxiv - Neuroscience 2023Quote: ... control shRNA (shCtrl) and Pgbd5 shRNA (shPgbd5) (Table S5) were cloned along the hU6 promoter in a pSilencer 3.0-H1 (ThermoFisher Scientific) plasmid using EcoRI and HindIII restriction sites and substituting the original H1 promoter ...
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bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes were transfected with lentiviral vectors expressing shRNAs (see “Lentivirus and retrovirus production” section below) using Lipofectamine 3000 reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Astrocytes were transfected with lentiviral vectors expressing shRNAs (see the “Lentivirus and retrovirus production” section below) using Lipofectamine 3000 reagent (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: Plasmids for microinjection were extracted using the PureLink HQ Mini Plasmid Purification Kit or HiPure Plasmid Midiprep kit (Invitrogen). Genomic DNAs were extracted from mix-staged animals with PureLink Genomic DNA Mini Kit (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA pool was isolated from each replicate using automated plasmid extraction using MagJET plasmid extraction kit (ThermoFisher Scientific). Barcode diversity assay kit from Inscripta was used to construct libraries containing barcodes from plasmid pool for each sample for sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... R01-09 ESCs were seeded on mouse embryonic fibroblasts (MEFs) and then transfected with the two designed plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Transfected cells were selected by transient treatment with 1 µg/ml puromycin for 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA encoding mouse FAK (Ptk2) without EGFP was PCR amplified from this plasmid and subcloned into pENTR/D-TOPO entry vector (Thermo Fisher Scientific). Entry vector is then further cloned into lentiviral destination vector pDest-659 along with mPol2 promoter and C-terminal myc-tag entry vectors using the method described above ...
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bioRxiv - Genomics 2021Quote: ... pools of mouse ES cells harboring integrated transposons were transfected with 5 μg of plasmid pCBASceI using Lipofectamine 2000 (Thermo Fisher, #11668027). The growth medium was changed after 16 h later ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells with either ATF6 plasmids or ATF6-mutant plasmids were transfected with 200 ng of FGF2-ERSE-luciferase-reporter plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... Lenti-virus expressing GATA4 or GATA6 was packed with the 2nd generation lentiviral packaging plasmids, psPAX2 (Plasmid #12260, addgene) and pMD2.G (Plasmid #12259, addgene) with Lipofectamine 2000 (Cat. 11668019, ThermoFisher) in HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep kit (Thermo K0503) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent) ...
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bioRxiv - Neuroscience 2019Quote: ... All plasmids were purified with the PureLink HiPure Plasmid Maxiprep Kit (Invitrogen), and then dissolved in PBS.
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bioRxiv - Microbiology 2021Quote: ... and plasmids were purified using the GeneJET Plasmid Miniprep Kit (Thermo Fisher). Purified plasmids were used as template for PCR using primers that discriminate between each flagellar switch sequence orientation ...
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bioRxiv - Synthetic Biology 2022Quote: All plasmids were extracted using the GeneJET Plasmid MiniPrep Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were recovered using Quick Plasmid Miniprep Kit (ThermoFisher, Waltham, MA, USA) and digested using SacII to verify the presence of the sgRNA ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid DNA isolation was performed using GeneJET Plasmid Miniprep Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated with the GeneJet plasmid miniprep purification kit (Thermo Fisher) per manufacturer protocols ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific) and verified by Sanger sequencing ...
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bioRxiv - Physiology 2020Quote: ... The recombinant plasmids were extracted with PureLink Quick Plasmid Miniprep Kit (Invitrogen) from successful clones.
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were extracted using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific), and then Sanger sequenced ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid DNAs were purified with PureLink® Quick Plasmid Miniprep Kit (Invitrogen), linearized with Spe1 restriction enzyme (New England BioLabs) ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid DNA was isolated using the HiPure Plasmid Maxiprep kit (Life Technologies) or the ZymoPURE Plasmid DNA Isolation Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2020Quote: ... The plasmids were isolated using the PureLink Quick Plasmid Miniprep Kit (ThermoFisher) and sequenced by ATGC Inc ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were routinely isolated using the GeneJET Plasmid Miniprep kit (Thermofisher, USA).
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bioRxiv - Biochemistry 2020Quote: ... Plasmid amplification was done using the PureLink HiPure Plasmid Maxiprep Kit (Invitrogen). All cloned plasmids were validated by LightRun sequencing (Eurofins genomics) ...