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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μg of hCas/hanCas plasmid and 0.5 μg of the corresponding sgRNA plasmid were transfected with 2 μl of Lipofectamine 2000 (Life Technologies) diluted in 100 μl of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 1 × 106 KH2 ESCs were electroporated with 5 μg of pBS31–TetON plasmid and 5 μg of pCAGgs–FLPe plasmid using the Neon system (Invitrogen) with two impulses (20 ms ...
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bioRxiv - Microbiology 2020Quote: ... Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco, 31985062) and a total final volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNA from ten randomly chosen and pooled CRISPR ON colonies was isolated using GeneJET Plasmid Miniprep Kit (Thermo scientific) and retransformed into fresh prepared CRISPR ON and CRISPR OFF cells (Fig ...
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bioRxiv - Microbiology 2019Quote: ... Standard curves for gfp and oriV were constructed using plasmid isolated from the ancestral strain using the PureLink Plasmid DNA Miniprep Kit (Invitrogen). Total DNA amounts were quantified using a Qubit Fluorometer (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
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bioRxiv - Genomics 2021Quote: ... pSB-CRISPR-sgL1 and pSB-CRISPR-sgAlu plasmids were transfected together with SB100X plasmid into the cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s protocol ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The lentivirus of gRNA or shRNA library was produced by co-transfection of library plasmids with two viral packaging plasmids psPAX and pMD2.G into HEK293 cells using Lipofactamine 2000 (Invitrogen). Huh7 cells were transduced with lentivirus libraries at multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). The cell culture supernatant clarified on day 6 and subjected to positive PGT145 antibody affinity chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant AAV6.2 vectors were produced by calcium phosphate transfection of human embryonic kidney (HEK)-293 cells using the pFB_CMV-hDTR or the pFB_stuffer plasmid in combination with a pAAV-Cap6.2 (56) and the pHelper plasmid (Thermo Fisher Scientific), followed by purification and titer determination as described previously (57).
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng or 1000 ng of E2 WT or E2−p53 plasmid DNA along with 1000ng of pTK6E2-Luciferase reporter plasmid using Lipofectamine 2000 according to the manufacturer’s instructions (ThermoFisher Scientific). Cells were harvested the next day using Promega luciferase assay system ...
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bioRxiv - Biochemistry 2022Quote: ... At least 1 × 106 colonies were scrape-harvested with LB broth and plasmids were extracted using a PureLink Plasmid Midiprep kit (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... Viral stock was generated by co-transfecting HEK 293T cells with 10:1 ratio of HIVGKO plasmid and a plasmid encoding VSV-G envelope using Lipofectamine 3000 reagent (Invitrogen). Culture supernatant was harvested 48hrs post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... R1-AD1 and R1-D567 cells were transfected with equimolar amount of pSpCas9-2A-Puro H3F3A-1-73 plasmid and pUC19-FLAG-HA-H3F3A plasmid using Lipofectamine 2000 (Invitrogen). After 3 days transfected cells were trypsinized ...
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bioRxiv - Microbiology 2023Quote: Plasmid isolation and DNA fragments purification was performed using the ThermoScientific GeneJET Plasmid Miniprep Kit (Thermo Scientific, Waltham, MA USA) and GeneJET Gel Extraction Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Microbiology 2023Quote: ... the spectinomycin cassette from the pAT28 plasmid [23] was amplified using primers 2836/2837 and cloned into the pBlunt2 plasmid (Invitrogen), giving rise to pTC129 ...
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bioRxiv - Immunology 2023Quote: ... The next day, plasmid (mCherry-hLC3B-pcDNA3.1, addgene plasmid #40827) transfection (800 ng/well) was performed with Lipofectamine 2000 (Invitrogen, 16688), following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Individual clones were inoculated in LB for overnight growth and plasmids were purified using GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific) and verified by Sanger sequencing (Azenta Life Sciences).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 100ng of FLAG-Ubiquitin-expressing plasmid and 200ng of HA-KDM5-epxressing plasmid using the Effectene II (Invitrogen) kit according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were co-transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP and different pKH011-puro guide plasmids together with the piggyBAC transposase plasmid via electroporation with the Neon Transfection System (ThermoFisher). Transfected cells were selected and maintained with 6 μg/mL Blasticidin and 6 μg/mL Puromycin ...
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bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were co-transfected with 200 ng of prime editor plasmid and 100 ng of pegRNA plasmid using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and 200 ng NICD-expressing plasmid (or empty pcDNA3.1(+) negative control plasmid) using Lipofectamine LTX with PLUS reagent (Life Technologies). Cells were lysed 44-48 hours post-transfection by adding 250 μL Passive Lysis Buffer (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... ApoER2 plasmids were co-transfected with RAP plasmid at a 1:2 ratio in Opti-MEM medium (Gibco, Thermo Fisher) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... were electroporated with 250 ng of sgRNA-encoding plasmid and 750 ng of Cas9 expression plasmid using a NEON system (ThermoFisher) at 1050 V for 30 ms (two pulses) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.8 μg each PBK/TECTAWT and PBK/TECTARS expression plasmids and 0.1 μg PCAGGS/TMPRSS2 plasmid were transfected to each well using 400 μl OptiMEM (Invitrogen, 31985070) and 2 μl Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T was co-transfected with lentiviral expression constructs (12 µg), viral packaging plasmid (psPAX2, 8 µg), and viral envelope plasmid (pMD2.G, 4 µg) using Lipofectamine 2000 (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were introduced using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were diluted in 1 ml OptiMem (Gibco) and 20 μl of PEI (1 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed with Lipofectamine 2000 (ThermoFisher) as recommended by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... plasmids were purified using the Purelink kit (Thermofisher). Unless noted otherwise ...
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bioRxiv - Genomics 2020Quote: ... plasmids using lipofectamine plus 2000 (Thermo Fisher Scientific). After 5h ...
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bioRxiv - Molecular Biology 2020Quote: ... This episomal plasmid contains a GateWay™ (Invitrogen) recombineering cassette that allows the cloning and expression of fungal effectors without a signal peptide (SP) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transfected with Lipofectamine 3000 (Invitrogen, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plasmids using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Cells were incubated for 24 to 48 hours in the transfection solution ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine3000 (ThermoFisher Cat #L3000001), while in Figure 1d and Supplementary Figure 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids were transfected using Lipofectamine2000 (ThermoFisher Cat #11668030), following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: Minigene plasmids were transfected using Lipofectamin 3000 (Invitrogen) with reverse transfection protocol for 24 hours followed by cells harvesting by trypsin-EDTA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid transfections were performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen). Selection was performed with G-418 (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... plasmids using the Neon Transfection System (Life Technologies). Forty-eight hours post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were generated using Gateway cloning (Life technologies) using the Drosophila Gateway Vector Collection (DGVC ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmid DNAs were purchased from GeneArt (ThermoFisher scientific), General biosystems Inc ...
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bioRxiv - Physiology 2019Quote: ... Indicated plasmids were transfected with Lipofectamine-2000 (Invitrogen) (for 36-42 hours) ...
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bioRxiv - Cell Biology 2019Quote: Plasmid transfection was performed with Lipofectamine2000 (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... after plasmid digestion with DraI FD (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were done with Lipofectamine 2000 (Invitrogen) following manufacturer’s instruction ...