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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Mouse ARHGAP31 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids containing the promoter regions were ordered via gene synthesis as Gateway-compatible donor plasmids (ThermoFisher Scientific). Promoter sequence and expression cassette from donor constructs pEntry.P4P1R.EF1a and pEntry.MiniCas9V2 (Maria et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pWS082-CAN1 sgRNA plasmid (Table S1) was extracted using the GeneJet Plasmid miniprep kit (ThermoFisher Scientific). Accuracy of the cloning was confirmed through DNA sequencing.
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bioRxiv - Cell Biology 2023Quote: ... The confirmed plasmid was prepared in large quantities using the PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). The plasmid concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was isolated using the GeneJET Plasmid Miniprep Kit (Cat #K0503; Thermo Fisher Scientific, MA, The US). Isolated plasmids were prepared for transformation by linearizing with EcoRI restriction enzyme according to the supplier’s instructions (NEB ...
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bioRxiv - Microbiology 2024Quote: Isolation of plasmid DNA was carried out with Genejet Plasmid Miniprep kit (Thermo Fisher Scientific, Pittsburgh, PA, USA), according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng firefly luciferase plasmid and 112.5 ng GFP or different eIF1 plasmids using Lipofectamine 2000 (Thermo Fisher). After 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 750 ng of plasmid purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Bioengineering 2020Quote: ... using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and were sequence verified ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500ng of T7 calmodulin plasmid (Invitrogen, #454002) was also used in the following cell free expression reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and Plus reagent (Invitrogen # 11514015) were mixed at 1:1 and incubated for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and PureLink HiPure Plasmid Midiprep Kit (Invitrogen). The DNA sequences were read by dye terminator cycle sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... and cloned into the pBAD plasmid (ThermoFisher), as previously described (46) ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were transfected using Lipofectamin 2000 (Invitrogen) according to the manufacturer’s instructions for 48 hours before harvesting ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were delivered using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids by Lipofectamine 3000 (Invitrogen, L3000015, USA). The relative hACE2 quantity was evaluated through mRNA expression level using following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into the pCR8 plasmid (Invitrogen), then transferred using the Gateway system into the pBPGUw (Pfeiffer et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were transformed into BL21(DE3)(Invitrogen). Bacterial cultures were grown to an OD600 of 0.6 at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... later sub-cloned in pet28a plasmid (Invitrogen) in order to produce 6 His-tagged proteins.
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bioRxiv - Bioengineering 2019Quote: ... derived from plasmid pYES1L (Thermo Fisher Scientific), was inserted into pRM01 ...
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bioRxiv - Immunology 2019Quote: ... into a modified pMT/BiP plasmid (Invitrogen) that encodes for a C-terminal strep-tag sequence ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid mixes were prepared in Optimem (Gibco) and mixed with 0.75 μl of LF2000 and added dropwise to cells ...
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bioRxiv - Cell Biology 2019Quote: ... for plasmid DNA or Lipofectamine RNAiMAX (Invitrogen) for siRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... reaction between pDONR221 plasmid (ThermoFisher Scientific, 12536017) containing V5-tagged wild-type or mutant CBL cDNA and lentiviral destination plasmid pRRL-SFFV-IRES-GFP or -dTomato (kindly provided by Christopher Baum and Axel Schambach ...
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bioRxiv - Genomics 2019Quote: ... Plasmids were transformed into GT115 E.coli (Invitrogen) and DNA isolated using PureYield Plasmid Midiprep system (Promega) ...
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bioRxiv - Bioengineering 2019Quote: ... and GeneJET Plasmid Miniprep (Thermo Fisher Scientific) or any other commercially available PCR and plasmid purification kits.
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bioRxiv - Bioengineering 2021Quote: The dual expression plasmid pFastBacDual (Invitrogen, USA) was purchased from Thermo Fisher Scientific China Co. ...
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bioRxiv - Microbiology 2020Quote: The following plasmids were used: pcDNA3 (Invitrogen), pSUMO1 (pcDNA3-HA-SUMO1 was a gift from Junying Yuan ...
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bioRxiv - Microbiology 2021Quote: ... cloned into pJet plasmid (Thermo Scientific, USA), and a mutation was verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
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bioRxiv - Cell Biology 2021Quote: ... The d1-eGFP plasmid was from Invitrogen, and constructed by cloning the d1-eGFP synthetic gene into a pcDNA3.3-TOPO vector backbone.
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bioRxiv - Cell Biology 2021Quote: ... pLP/VSVG DNA plasmids (Thermo Fisher Scientific). Cell culture medium containing lentivirus particles was collected after 48 and 72 h ...
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bioRxiv - Cell Biology 2020Quote: ... Control plasmid pcDNA3.1 was purchased from Invitrogen. DDI2-V5-His (wild type ...
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bioRxiv - Cell Biology 2022Quote: ... and pCMV-Renilla luciferase plasmid (Thermofisher #16153). Cells were transiently transfected with PTPN14 V5 tagged expression constructs (Liu et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were transfected using Lipofectamine 3000 (ThermoFisher) per manufacturer instructions ...
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophybioRxiv - Neuroscience 2022Quote: ... plasmids were performed with Lipofectamin 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... into the pENTR-D-TOPO plasmid (Invitrogen) and perform an LR clone reaction to transfer it into the destination lentiviral vector SIN-cPPT-PGK-Gateway-WPRE-miR124T plasmid ...
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bioRxiv - Plant Biology 2021Quote: ... and transferred to pK2WG7 plasmid (Thermo Fisher), both previously digested using restriction enzymes ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were diluted in NeuroBasal Plus (Gibco) media so that 0.5-1 µg of DNA would be added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid was synthesised by GeneArt (ThermoFisher), cut with SfiI ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... pDONR-221 and pDONR–P2RP3 plasmids (Invitrogen). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmids were transfected with Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s instruction at a final concentration of 1 µg in a 60 mm dish ...
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bioRxiv - Synthetic Biology 2021Quote: ... of each plasmid and using pUC19 (ThermoFisher) as DNA “stuffer” ...
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bioRxiv - Cell Biology 2021Quote: ... PureLink™ HiPure Plasmid Miniprep Kit (Invitrogen) was used for low endotoxin plasmid preparation ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacture provided protocol.
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were transfected with Lipofectamine 2000 (Invitrogen) according to the vendor’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: All plasmids were transfected using Lipofectamine (Invitrogen) following standard protocols ...
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bioRxiv - Microbiology 2022Quote: ... plasmid DNA was linearized with NotI (Invitrogen) and SP6 RNA polymerase (NEB ...