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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Mouse ANKRA2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Plasmid DNA from both the ETEC strains was additionally isolated using the Gene Jet Plasmid Miniprep Kit (Thermo Scientific) by following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... ES cells were transiently transfected with 15 µg of pAC94 plasmid DNA and 1.25 µg each of the four different pmU6 plasmids using Lipofectamine 3000 and OptiMEM (Gibco). Cells were seeded on a 15-cm plate 24 h before transfection and grown in ES media without penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was isolated and purified from the Stbl3 cells using the HiPure plasmid kits from Invitrogen (Fischer scientific). This TRE-LSL plasmid was then opened up using restriction enzymes XbaI and EcroI (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... were co-transfected with the indicated 400ng pGEMT plasmid and 600ng pSiren plasmid per well using Lipofectamine 2000 (Invitrogen). 24 h later ...
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bioRxiv - Immunology 2021Quote: ... 293T Cells were transfected with the indicated Spike expression plasmids or a control plasmid using Lipofectamine 2000 (Life technologies). One day after ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid contains the CFTR gene coding sequence in the pCDNA5 FRT/TO plasmid backbone (Life Technologies, Carlsbad, CA). The CFTR coding sequence with a C-terminal T7 tag from pcDNA5.FRT-wtCFTR was cloned into the multiple cloning site of the pCW57-GFP-2A-MCS plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... were amplified as described for the protein expression plasmids and cloned BamHI/EcoRI into an in-house modified pcDNA3.1+ plasmid (Invitrogen) with an N-terminal FLAG tag cloned NheI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B or HEK293T cells at ∼80% confluency were transfected with Cas9 plasmids (0.6 μg) and two HS5-1-NRSE-targeted sgRNA-expressing plasmids (1.2 μg) by Lipofectamine 2000 (Life Technologies) in a 12-well plate ...
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bioRxiv - Molecular Biology 2022Quote: 2nd generation lentiviral transfer constructs containing the SFFV promoter upstream of CFTR with and without the transgene separated by a self-cleaving T2A peptide packaging plasmid (pMD2.G) and envelop plasmid (pCMVR8.74) were packaged into HEK293T cells via co-transfection with lipofectamine 2000 (Thermofisher) (25) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E ...
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bioRxiv - Molecular Biology 2022Quote: ... From this collected bacterial culture plasmids were extracted using the PureLink™ HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pAAV-CMV-tKiMBI-T2A-caMEK-bGH_pA plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then were sent to Stanford Gene Vector and Virus Core to produce and titer AAV.DJ vectors.
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bioRxiv - Biophysics 2023Quote: ... The gRNA plasmid and the replacement plasmid were introduced into HeLa cells using the Neon transfection system (ThermoFisher Scientific). GFP-positive cells were sorted and plated in 96-well plates as a single cell ...
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bioRxiv - Microbiology 2023Quote: ... transfection complexes were prepared by diluting 2 µg of plasmid (or for co-transfections, 1µg of each plasmid) in 100µL OptiMEM (Gibco, #31985062), mixed with 4 µL of FuGENETM HD and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1.25 μg of each pcDNA5/FRT/TO/i/FLAG/MCP-based plasmid and 1.25 μg of pOG44 plasmid encoding recombinase were transfected using Lipofectamine LTX (Invitrogen). Cell lines with stably integrated constructs were selected with 150 μg/ml hygromycin (Wako) ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µg packaging plasmid (psPAX2) and 2.5 µg envelope plasmid (pMD2.G) using Lipofectamine 2000 in OptiMEM (ThermoFisher Scientific). After 6-hr incubation of Lipofectamine/DNA mixture in OptiMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated by the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientific, Waltham, MA), and precipitated onto gold particles (0.6 µm ...
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bioRxiv - Biophysics 2023Quote: ... with 10-100 ng of the K2P2.1 plasmid and 400 ng of an eGFP plasmid (for visualization) using LipofectAMINE 2000 (Invitrogen) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... CRISPR plasmids and pEGFP-C1 puromycin plasmids were co-transfected in HeLa cells using lipofectamine LTX reagent (ThermoFisher, 15338030), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... To standardize the mass of transfected plasmids among transfection experimental conditions we added a filler pcDNA3.1 “empty” plasmid (Thermofisher).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were co-transfected with 0.5 μg of the corresponding pcDNA5 plasmid and 4.5 μg recombinase plasmid POG44 (Invitrogen V600520). After 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.84 μg of pCMV-VSV-G envelope plasmid, 5.67 μg of pCMV dR8.2 dvpr packaging plasmid in OptiMEM medium, Gibco #31985062) and 1 ml of premix 2 (42.5 μl of Lipofectamine LTX ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube ...
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bioRxiv - Bioengineering 2024Quote: ... 300 ng of dCas13 expression plasmid was mixed with 300 ng of rcRNA expression plasmid and 300 ng of splitGFP reporter plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube 3.2 µl of polyethylenimine (Polysciences ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Bioengineering 2021Quote: ... and transfected a day later with 200 ng of LSR plasmid and 178 ng of pseudosite attD plasmid using Lipofectamine 2000 (Invitrogen). As a non-matching LSR control ...
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bioRxiv - Genetics 2019Quote: ... For deletions MEL745a cells were transfected with one plasmid targeting upstream and one plasmid targeting downstream sequence surrounding the enhancer using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid pCR8-pilA1-ORF was recombined with plasmid pAX1-GW(12) (Table 3) using the Gateway LR recombination protocol (Invitrogen). The resulting plasmid ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Systems Biology 2019Quote: ... cells were co-transfected with 10 ng of pMiRCheck2 miRNA sensor plasmid and 500 ng of individual pKT2-Fah-TuDstd or pKT2-Fah-TuDtail plasmids using Lipofectamine 3000 Reagent (Invitrogen). Cultures were harvested 24 h post-transfection and processed for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Genetics 2019Quote: ... grown overnight and plasmid DNA was extracted using the PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA, USA). Plasmids were Sanger sequenced using the M13F sequencing primer and correct sgRNA sequences were identified using a BLAST alignment of the sgRNA oligos ...
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bioRxiv - Microbiology 2021Quote: ... Positive transformants were confirmed by PCR and the plasmid was extracted using a GeneJET Plasmid Miniprep Kit (Thermo Scientific™). Electrocompetent cells of Pseudomonas aeruginosa (PAO1 ...
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bioRxiv - Genomics 2021Quote: ... 1×105 cells were electropotated with 250 ng of pegRNA-encoding plasmid and 83 ng of sgRNA-encoding plasmid (for PE3) using a NEON system (ThermoFisher) at 1,050 V 30 ms (two pulses) ...
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Neuroscience 2019Quote: ... Two million cells were transfected with 2.5 μg of each gRNA-encoding plasmid and 4 μg of donor plasmid using the Neon Transfection system (Life Technologies). A GFP puromycin-free plasmid was used as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... The cloned plasmids were transfected in to HEK293T cells in combination with pSPAX2 and pCMV-G plasmids using Lipofectemine 2000 (Invitrogen). Supernatants were collected at 24 and 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with a total of 15 μg DNA expression plasmids (7.5 μg of each plasmid in co-transfection assays) using Lipofectamine 3,000 (Thermo Fisher Scientific). After 24 h of transfection ...
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bioRxiv - Immunology 2021Quote: ... within a pEX-K4 (EtAMA1Cit) or pMK-RQ (EtIMP1Cit) plasmid with appropriate restriction enzyme sites for cloning into pYD1 yeast display plasmid vector (Invitrogen, Thermofisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... 15 x104 cells were harvest and co-transfected with 0.1 µg control plasmid Renilla and 5µg reporter plasmid Firefly (Neg or Mut) via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... In transient transfection of HEK293FT cells with pRK5-TGFβRI-FLAG plasmid (#14833) or pCMV5B-TGFβRII plasmid (#11766) using Lipofectamine 3000 transfection kit (Invitrogen, #L3000-008). For lentivirus production ...
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bioRxiv - Cancer Biology 2022Quote: ... the cloned plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Invitrogen) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of pig fibroblast cells were performed by electroporation of wildtype large white pig fibroblast cells with a plasmid encoding Piggybac transposase and pBv1-EF-6X plasmid using Neon Transfection System (Invitrogen) under condition of 1450 V ...
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bioRxiv - Cancer Biology 2022Quote: H-1299 and H-23 cells were transfected with plasmids or co-transfected with plasmids and siRNAs using Lipofectamine 2000 (Invitrogen) as in the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: HEK293FT cells were transfected with the plasmid mix (plasmid of interest, PPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher) as a transfection reagent diluted in Opti-MEM (Thermo Fischer) ...
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bioRxiv - Neuroscience 2022Quote: ... each retroviral or lentiviral backbone plasmid was co-transfected to 293T cells with helper plasmids for retrovirus or lentivirus using Lipofectamine 2000 (Invitrogen). Culture medium was collected ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with Lenti-pCDH-CSP or Lenti-pCDH control plasmids with psPAX2 and pMD2.G packaging plasmids as ratio 20:15:5 using Lipofectamine 3000 (Invitrogen) to obtain the lentivirus ...