Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Mayaro Virus E2 Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... and Mayaro virus (MAYV, TRVL 15537) were propagated in Aedes albopictus C6/36 cells grown in Minimum Essential Medium (MEM, Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 (SARS-CoV-2/CANADA/VIDO 01/2020 ...
-
bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
-
bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific, E2-695), 5 mM adenosine triphosphate ...
-
bioRxiv - Systems Biology 2022Quote: ... PGE2 (prostaglandin E2) (ThermoFisher), EGF (epidermal growth factor ...
-
bioRxiv - Cell Biology 2023Quote: ... Prostaglandin E2 level was measured and standardized with protein level using BCA Protein Assay Kit (Thermo Fisher, #23227).
-
bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
-
bioRxiv - Neuroscience 2021Quote: ... prostaglandin E2 (PGE2) (Thermo Fisher, St. Louis, MO) and CB-13 were dissolved in DMSO and diluted in external recording solution.
-
bioRxiv - Bioengineering 2021Quote: ... stained with a monoclonal antibody (Invitrogen,15G5A11/E2) against rat β-actin at a dilution of 1:2000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and β-Estradiol (E2; Acros Organics, CAT: 436320050) served as a positive control ...
-
bioRxiv - Immunology 2021Quote: ... The lentiviral envelope vector pLP/VSVG (Invitrogen) and the packaging vector psPAX2 (Trono lab ...
-
bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
-
bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
-
bioRxiv - Cancer Biology 2020Quote: ... wtCCDC170 or the ΔCCDC170 fusion variants E2-E7 or E2-E10 was performed using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
-
bioRxiv - Bioengineering 2023Quote: ... 1nM or 10nM E2 dissolved in ethanol (Thermo Fisher). E2 was replenished every 24 hours and media was changed every 48 hours ...
-
bioRxiv - Biochemistry 2019Quote: Antibodies and antibody Fabs were produced as described previously.57 BG505 N332 SOSIP gp140 envelopes were expressed as previously described with minor modifications.58 Envelope production was performed with Freestyle293 cells (Invitrogen). On the day of transfection ...
-
bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... the VSV-G envelope protein vector pMD2.G and the respective pGIPZ-shRNA-target plasmid using lipofectamine 3000 (Invitrogen #L3000008). Filtered virus supernatant was used to transduce MDA-MB-468 and 4T1 cells in DMEM supplemented with 2% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... CBS-E2 cargos were labeled with DyLight 550 (ThermoFisher, USA), incubated with or without unlabeled TAT-CaM in equimolar amounts and brought to a total volume of 50 μL in binding buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pellets were resuspended in 100µl electrolyte buffer E2 (Thermo Fisher #MPK10096) with 3µg CRISPR/sgRNA plasmid (Addgene #48139 ...
-
bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
-
bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 260 ng pMD.G envelope plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.25 µg envelope plasmid diluted in 100 µl optiMEM (Gibco) was mixed with 20 µl of PolyFect transfection reagent (Qiagen ...
-
bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
-
bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
-
bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
-
bioRxiv - Biophysics 2022Quote: ... and pCMV-VSV-G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
-
bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
-
bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
-
bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
-
bioRxiv - Immunology 2024Quote: ... The lentiviral envelope vector pLP/VSVG was purchased from Invitrogen (Invitrogen/ life technologies) and the packaging vector psPAX2 was a kind gift from Didier Trono (Addgene plasmid # 12260).
-
bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
-
bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
-
bioRxiv - Molecular Biology 2019Quote: ... 100 nM E2 or ethanol and 10 µM of 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 24h ...
-
bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
-
bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
-
bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
-
bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
-
bioRxiv - Microbiology 2022Quote: ... Cell envelope samples were vitrified using a Vitrobot plunge-freezing machine (Mark IV, ThermoFisher) at room temperature (blot force 2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the cell envelope was stained with wheat germ agglutinin-AlexaFluor488 (WGA-AF488, Life Technologies), which was added to the loading well to a final concentration of 10 μg/mL prior to loading cells into the imaging chamber ...
-
bioRxiv - Molecular Biology 2020Quote: ... pMD2-VSVG (envelope vector) and pCDH plasmids co-transfection using Lipofectamine 2000 (#11668019, Invitrogen). Medium containing virus was harvested 48 h after transfection and filtered by 0.45 μm millipore filter ...
-
bioRxiv - Genomics 2022Quote: ... psPAX2 packaging plasmids and pMD2.G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s protocol ...