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Citations for Thermo Fisher :
151 - 200 of 6237 citations for Malate dehydrogenase cytoplasmic MDH1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Systems Biology 2020Quote: ... BHKs were additionally stained with 1uM CellMask Red CMTPX cytoplasmic dye (Invitrogen C34552) for 10min at room temperature followed by three washes with PBS prior to imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... cytoplasmic and nuclear fractions were isolated and RNA extracted using PARIS kit (ThermoFisher). The resulting RNA was DNase treated and cDNA generated as above ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cytoplasmic faction was treated with RNase A (Invitrogen, Waltham, MA, USA) at a final concentration of 20 mg/ml and incubated for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclear fraction was isolated using NE-PER Nuclear and Cytoplasmic extraction kit (ThermoFisher). Equal protein amounts of nuclear cell lysates were run on 4-20% gradient Tris-glycine gels (Novex-Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear and cytoplasmic fractions were isolated using the NE-PER kit (Thermo Scientific), as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or NE-PER ™ Nuclear and Cytoplasmic Extraction Kit (78833, Thermo Scientific, UK) were used to isolate total protein or cytoplasmatic/nuclear protein fractions for western blotting ...
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bioRxiv - Microbiology 2019Quote: ... An aliquot of cytoplasmic lysate was immediately stored in TRIzol LS reagent (Ambion) for extraction of total RNA ...
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bioRxiv - Immunology 2019Quote: ... and a cytoplasmic-staining dye (Vybrant CFDA SE Cell Tracer Kit, Life Technologies). The double-labeled cells were coated with a clade A gp120 (BL035.W6M.Env.C1 ...
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bioRxiv - Cell Biology 2021Quote: ... Both of the cytoplasmic and nuclear fractions were then resuspended in Trizol (Invitrogen) and RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: The fractionation was performed using NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher) as manufacture instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Both cytoplasmic and nucleus fractions were treated with 2X lysis buffer (Molecular Probes) and boiled at 95°C for 10min ...
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bioRxiv - Cancer Biology 2022Quote: Nucleo-cytoplasmic fractionation was performed using the PARIS kit (Ambion, ThermoFisher Scientific, AM1921) based on the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Nucleo-cytoplasmic fractionation was performed using the PARIS kit (Ambion, ThermoFisher Scientific, AM1921) based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCDH1 was detected using a polyclonal antibody targeting the PCDH1 cytoplasmic tail (ThermoFisher).
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bioRxiv - Cancer Biology 2023Quote: Cells were first fractionated using the Nuclear and Cytoplasmic Extraction kit (Thermo Scientific). The resultant cytoplasmic and nuclear lysates were incubated with the corresponding beads for 2 h rotating at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... NE-PER Nuclear and Cytoplasmic Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) was used to separate the nuclear and cytoplasmic fractions per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Cancer Biology 2021Quote: Lactate dehydrogenase (LDH) cytotoxicity assay was performed using the assay kit (Invitrogen, C20301) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... VIM (Hs00958111_m1) and glyceraldeyde-3-phosphate dehydrogenase (GAPDH) (Hs02758991_g1) were purchased from ThermoFisher Scientific ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... Mouse monoclonal antibodies against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (# 4300) were from Ambion. Secondary antibodies conjugated with horseradish peroxidase were from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2023Quote: ... A rodent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primer and probe set (Applied Biosystems) were included in each reaction to normalize the DNA added ...
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bioRxiv - Microbiology 2023Quote: ... the cellular glyceraldehyde 3-phosphate dehydrogenase (GADPH) with SuperScriptTM III Reverse Transcriptase (Invitrogen) and quantified using SYBR-Green (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using CyQuant lactate dehydrogenase (LDH) cytotoxicity assay (ThermoFisher Scientific) in a 96-well plate.
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Systems Biology 2021Quote: ... malate and tartrate) and the main fermentative by-products (ethanol, glycerol and 2.3 butanediol) were quantified using HPLC (Thermo Fisher Scientific, Waltham, MA) coupled with refraction index and UV/VIS (210 nm ...
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bioRxiv - Systems Biology 2021Quote: ... malate and tartrate) and the main fermentative by-products (ethanol, glycerol and 2.3 butanediol) were quantified using HPLC (Thermo Fisher Scientific, Waltham, MA) coupled with refraction index and UV/VIS (210 nm ...
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bioRxiv - Developmental Biology 2020Quote: ... NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific, Waltham, MA, USA, #78833) were used.
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bioRxiv - Developmental Biology 2020Quote: ... Nuclear and cytoplasmic fractions were isolated using the NE-PER kit (Thermo Scientific 78833) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... RNA was isolated using SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit (Fisher Scientific) as described earlier [9].
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagent (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclear and cytoplasmic fractions were purified by PARIS Kit (Ambion, Life Technologies, USA). RNA was reverse transcribed by HiScript II Q RT SuperMixfor qPCR (+gDNA wiper ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclear and cytoplasmic fractions were purified by PARIS Kit (Ambion, Life Technologies, USA). RNA was reverse transcribed by HiScript II Q RT SuperMixfor qPCR (+gDNA wiper ...