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Citations for Thermo Fisher :
501 - 550 of 6237 citations for Malate dehydrogenase cytoplasmic MDH1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... we prepared nuclear and cytoplasmic fractions from untreated cells using a PARIS™ kit (Thermo Fisher Scientific, cat# AM1921).
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bioRxiv - Genetics 2024Quote: ... The remaining pellet was collected as the chromatin-associated fraction.Total RNA from the cytoplasmic and nuclear fractions was extracted by using PureLink RNA MiniKit (Invitrogen) following the manufacturer’s instructions for the RNA extraction from aqueous solutions ...
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: Lysate Preparation: DF1 and HeLa cells were fractionated using the NE-PER Nuclear and Cytoplasmic Extraction kit (ThermoFisher Scientific). All steps and buffers used were performed on ice or at 4°C unless otherwise stated ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Physiology 2020Quote: ... Explant viability was assessed by measuring lactate dehydrogenase (LDH) release using the Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) follow manufacturer’s instructions and tissue was either snap-frozen for RNA and protein extractions or fixed in 4% w/v PFA and paraffin embedded for downstream analyses.
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bioRxiv - Neuroscience 2022Quote: ... assay ID: Mm00433832_ml) as well as for the housekeeping gene Glycerinaldehyd-3-phosphat-Dehydrogenase (GAPDH; endogenous control; Life Technologies) that was labeled with another fluorescent dye ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was removed and used to quantify lactate dehydrogenase release using the CyQuant LDH assay kit (Invitrogen, Cat#C20300) following the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Microbiology 2021Quote: ... and subjected to the nucleus and cytoplasm extraction using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Cat. #78833, Thermo Scientific) following the manufacturer’s instructions and our previous studies (62) ...
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bioRxiv - Physiology 2019Quote: ... 100 mg of tissue was homogenized using 10 strokes in a 2 ml ground glass dounce-type tissue grinder in the buffers provided in the NE-PER nuclear and cytoplasmic extraction kit from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane and cytoplasmic fractions were separated using the Mem-PER™ Plus Membrane Protein Extraction Kit (ThermoFisher Scientific, Cat #78833), according to manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... Untreated and cytokine treated MIN6 nuclear extracts (NEs) were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents as per manufacturer’s recommendation (78833, ThermoFisher Scientific), supplemented with 1x protease inhibitors (40694200 ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular RNA or the RNA of cytoplasmic and nucleus fractions were extracted using the manufacturer’s protocol of TRIzol reagent (15596026, Thermo Scientific) or TRIzol LS Reagent (10296010 ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular RNA or the RNA of cytoplasmic and nucleus fractions were extracted using the manufacturer’s protocol of TRIzol reagent (15596026, Thermo Scientific) or TRIzol LS Reagent (10296010 ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with Halt protease inhibitor cocktail [Thermo Fisher Scientific]) to obtain whole-cell lysates or lysed using the NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific) to obtain cytoplasmic and nuclear fractions ...
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bioRxiv - Genomics 2019Quote: ... Nuclear extracts were prepared from actively growing melanoma cells (UACC2331) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific). Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in PBS and fractionated using the NE-PER™ Nuclear and Cytoplasmic Extraction Kit following the manufacturer’s protocol (ThermoFisher).
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bioRxiv - Developmental Biology 2019Quote: Cytoplasmic and nuclear fractions of heart tissues were generated using the Subcellular Protein Fractionation Kit for Tissues (Life Technologies # 87790) with modifications ...
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bioRxiv - Cell Biology 2020Quote: The nuclear and cytosolic protein lysates were prepared by using the NE-PER Nuclear and Cytoplasmic Extraction kit (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Cytoplasmic and nuclear extracts were isolated using a nuclear extraction kit according to the manufacturer’s protocol (Affymetrix; Santa Clara, CA) with modifications43 ...
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bioRxiv - Pathology 2021Quote: ... pellets were resuspended in 50μl of 5% SDS containing phosphate buffered saline and cytoplasmic fractions were mixed 1:1 with 10% SDS solution (Thermofisher) to give a final 5% SDS concentration ...
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bioRxiv - Microbiology 2021Quote: ... cytoplasmic proteins and the total cell lysate proteins were denatured and boiled with 4× LDS sample buffer (Cat. #NP0007, Invitrogen) and subjected to immunoblotting analysis with equal protein loading of extracts (~20 µg/lane) ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were obtained from pelleted cells with the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, 78833) following instructions from the manufacturer and using protease and phosphatase inhibitors (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from cells in culture in nuclear and cytoplasmic fractions which were separated using the PARIS kit (Ambion) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... either from whole cells or from nuclear or cytoplasmic fractions was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treated with DNase I (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... either from whole cells or from nuclear or cytoplasmic fractions was extracted using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treated with DNase I (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were first incubated with a mouse anti-E-cad cytoplasmic domain mAb (1:1000) (4A2C7, Life Technologies, USA) for 1 h at room temperature and then with 1:500 rabbit polyclonal anti-C ...
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bioRxiv - Physiology 2023Quote: ... mouse liver tissue or HepG2 cells were separated with NE-PER Nuclear and Cytoplasmic Extraction Reagents (#78833, Thermo Fisher Scientific) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... subcellular protein extracts were obtained by using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Cat #78833, Thermo Fisher Scientific). Transfected cells were washed with PBS and trypsinized for 1 min using Trypsin-EDTA solution (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were subjected to lysis using NE PER Nuclear and Cytoplasmic extraction kit (Thermo Fisher Scientific Inc, Grand Island, NY) and proteins were extracted following manufacturer guidelines.
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bioRxiv - Immunology 2023Quote: AML and MDM cells were lysed with NE-PER Nuclear and Cytoplasmic Extraction kit according to the manufacturer’s protocol (Thermo Scientific). Western blot was performed and the membranes were incubated with the primary antibody for PPAR-γ ...
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bioRxiv - Genetics 2023Quote: ... with 8ug of LCL nuclear extract prepared using the NE-PER Nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific, 78833) and 30 ng of recombinant purified ELK1 (Sino Biologicals 12621-H20B) ...
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bioRxiv - Neuroscience 2023Quote: ... Lumbar spinal cords were dissected in oxygenated artificial cerebrospinal fluid and immediately homogenized using Cytoplasmic Extraction Reagent Kit from the NE-PERTM nuclear extraction kit (ThermoFisher) with a protease inhibitor cocktail added (5mg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into pcDNA3.1D/V5-His-TOPO (Thermo Fisher Scientific). MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS) (Gibco) at 37°C in the presence of 5% carbon dioxide (CO2) ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... for over-expression or pcDNA4/TO/myc-his-B (Thermofisher #V103020 ...
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 400 nM FITC-labeled TRAP220 or NCoR peptide in a buffer consisting of 20mM potassium phosphate (pH 8) ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 5 nM Fluormone Pan-PPAR Green fluorescent tracer ligand (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...