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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Macrophage metalloelastase MMP12 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The enriched fraction of airway macrophages was removed using Accutase (Gibco) and slow-frozen in two equal aliquots of freezing medium (90% FBS and 10% DMSO) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from macrophages were isolated using the TRIzol reagent (Invitrogen) following the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2021Quote: ... macrophage-colony stimulating factor (M-CSF) (50 ng/ml, ThermoFisher, PMC2044) was added to the medium and cells were cultured for an additional 2 days ...
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bioRxiv - Neuroscience 2023Quote: ... Differentiated macrophages were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, LMRNA015) with 20µM BHLHE40 siRNA (L-010318) ...
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bioRxiv - Genetics 2022Quote: ... Recombinant human macrophage colony-stimulating factor (CSF1, Gibco-Thermo Fisher, PHC9501) was then added at a final concentration of 100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and macrophage colony-stimulating factor (M-CSF, ThermoFisher, 203-IL-050). After 4 weeks ...
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bioRxiv - Genetics 2022Quote: ... Recombinant human macrophage colony-stimulating factor (CSF1, Gibco-Thermo Fisher, PHC9501) was then added at a final concentration of 100 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... and the macrophages were harvested using versene (Gibco, Cat. #15040-066) after day 10 when the cells were adherent and elongated ...
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bioRxiv - Immunology 2023Quote: RAW264.7 macrophage-like cell line was cultured in RPMI medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: Differentiated macrophages were passaged with 0.5% Trypsin-EDTA (Gibco, 25300-054), and plated onto inserts at 2-3×104 cells/well in 400 μl of differentiation media ...
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bioRxiv - Cell Biology 2024Quote: ... macrophages were harvested by trypsinization (0.25 % with 1mM EDTA, ThermoFisher 25200056) and plated at a density of 25,000 macrophages per well in an 8-well imaging chamber (Ibidi 80821) ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... The blots containing yeast Nap1 were treated similarly but incubated with mouse anti-HIS primary antibody (Thermo Fisher Scientific, Waltham, MA) and HRP-conjugated sheep anti-mouse secondary antibody (Kindle biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
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bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
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bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
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bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
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bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: 10,000 HEK293 FRT were seeded in 96 well plates (M33089, ThermoFisher UK) and induced with tetracycline for 24 hours ...
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bioRxiv - Genomics 2019Quote: Total RNA was prepared from harvested knockdown HEK293 cells using TRIzol (ThermoFisher). Single-stranded DNA in the sense orientation was synthesized from total RNA with random priming using the GeneChip WT Amplification kit (Affymetrix) ...