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Citations for Thermo Fisher :
551 - 600 of 6270 citations for MAX Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... monoclonal mouse anti-6x-His antibodies (Epitope-tag-clone: HIS.H8, Thermo Fisher) were employed ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS; Gibco™), 1% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The His-tagged protein was purified with Nickel-NTA resin (Thermo Scientific) in 20 mM Sodium Phosphate (pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... GBA was cloned into pcDNA3.1/myc-His A vector (Thermo Fisher Scientific) after digestion with EcoRI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT2 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS, Life Technologies), 2 mM GlutaMax (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Microbiology 2021Quote: ... Dynabeads™ His-Tag isolation and pulldown were purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT4 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Biophysics 2022Quote: ... blots were incubated with 6x-His Tag Monoclonal Antibody (Thermo Fisher Scientific). As a control ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122). Cells were maintained in humidified incubators at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
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bioRxiv - Neuroscience 2022Quote: ... and desmin were inserted into expression vectors pcDNA6 V5-His A (Invitrogen) using BamHI/XhoI ...
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bioRxiv - Microbiology 2022Quote: ... which is RPMI-1640 media containing 10% heat-inactivated (HI)-FBS (Gibco) and 1x penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678) and 1X TBE Running Buffer ...
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher), and rabbit polyclonal anti-mCherry (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... His-VASP was bound to HisPur™ Ni-NTA Resin (ThermoFisher #88221), washed twice with 5 CV of wash buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Immunology 2023Quote: His-tagged proteins were purified with HisPurTM Ni-NTA resin (Thermo Fisher). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Molecular Biology 2020Quote: ... Non-targeting control siRNA and siRNA targeting USP11 were transfected into cells using Lipofectamine RNAi MAX (Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Genomics 2020Quote: ... GATA6 lentivirus vectors were produced by using the LV-MAX Lentiviral Production System (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... HeLa cells were transfected with a mix containing siRNA (20 nM) and RNAi Max reagent (Life Technologies) according to the reverse transfection protocol then collected 72 h after for analysis ...
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bioRxiv - Genetics 2020Quote: ... and cloned into SfiI-digested pGL4.10M vector10 using One Shot MAX Efficiency DH5-T1R Competent E.coli (ThermoFisher). Plasmids were purified using Plasmid Plus Maxi kits (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with siRNA (control siFF and siUHRF1) and RNAi Max according to manufacturer’s protocol (Invitrogen). After 48 hours of siRNA treatments ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of bacterial cultures were pelleted for RNA extraction with Max Bacterial Trizol kit® (Invitrogen) according to manufacturer protocol.
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bioRxiv - Microbiology 2020Quote: ... ThermoScientific) using a Dionex Ultimate 3000 HPLC coupled to a Quantum Access Max mass spectrometer (Thermo Scientific) and run in the SRM negative scan mode ...
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... or 5 nM Silencer select siRNAs were transfected using Lipofectamine RNAi max transfection reagent (Thermo Fisher Scientific) at the same time as cell seeding ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid was used to transiently transfect FreeStyle 293F cells using FreeStyle MAX reagent (Thermo Fisher Scientific). The S ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) ...
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bioRxiv - Immunology 2022Quote: ... centrifugation (table top max speed; 5 mins) before they were run on 4-12% polyacrylamide gels (Invitrogen). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... and 8% CO2 following the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific). 20 ml of cells at a concentration of 106 cells/ml were transfected with 25 µg of DNA constructs ...