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Citations for Thermo Fisher :
501 - 550 of 7134 citations for M CSF Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xHJURP truncations or mutants in CSF-arrested or interphase extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Washed PBMC was then replenished with 5 ml of R10 supplementing with 2,000 U/ml of rh GM-CSF (ThermoFisher Scientific) and 4,000 U/ml of rh IL-4 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... 2×107 murine bone marrow progenitors were seeded in 100 ml RPMI-FBS supplemented with 10% J558-conditioned medium containing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in 500 cm2 square petri dishes (Nunc). Cells were incubated at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... were coated with capture antibodies diluted in 1X PBS: anti-mouse GM-CSF Capture (Invitrogen, #88-7334-88, 1:250), anti-mouse IL-10 capture (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SART1 was depleted from CSF extracts by 2 rounds of incubation with 60% (vol/vol) Dynabeads Protein A (Invitrogen) coupled with Xenopus SART1 antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Monocytes were differentiated into GM-CSF-polarised (M1) MDMs over seven days in RPMI 1640 media supplemented with 1 mM L-glutamine (Gibco), 2 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2023Quote: ... γ-TuRC was biotinylated prior to elution by incubation for 1 hour on ice in CSF-xB 2% (w/w) sucrose supplemented with 40 μM NHS-PEG4-biotin (ThermoFisher). The elution was concentrated using a 100 kDa MWCO spin concentrator (Amicon) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of CSF were loaded and separated by electrophoresis in a NuPAGE 4–12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250 mA ...
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bioRxiv - Systems Biology 2023Quote: 1μL of plasma and 150μL of CSF were depleted with depletion columns (High Select™ Depletion Spin Columns, A36370, ThermoFisher). The filtrate was collected and evaporated to dryness on SpeedVac (50 °C) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Microbiology 2024Quote: Extracted ion chromatograms of the [M-H]- (dA, dT, dC, 5hmdC, 5fdC, 5cadC)/[M+H]+ (5ghmdC) forms were integrated using Tracefinder software (ThermoFisher Scientific, MA, USA). Relative abundance in each sample was calculated by normalizing the peak area of each peak by the peak area of the dG signal in each specific sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: VeroE6 cell line (ATCC® CRL-1586™) was maintained in DMEM cell culture medium with the addition of 10% m/m Fetal Bovine Serum (FBS, Gibco/ThermoFisher, CA, USA) supplemented with 50 U/ml penicillin and 5 µl/mL streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed twice with TBS for 5 m and incubated for 30 m with 0.3% Triton-X (Fisher Scientific, Cat. #9002-93-1) in tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2022Quote: ... A 100 mM stock solution (in 0.2 M NaOH and 15% DMSO) of TCO*A-Lys was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630056), and added to the medium at a final concentration of 250 μM ...
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bioRxiv - Neuroscience 2021Quote: ... a stock solution of UAA (100 mM in 0.2 M NaOH containing 15% DMSO) was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630080) and added to cells to a final concentration of 250 µM for TCOA-Lys and TCO4en/eq-Lys ...
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Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis IIbioRxiv - Cell Biology 2020Quote: ... mRNAs for injection into GV or CSF arrested metaphase II oocytes were transcribed using the mMessage mMachine T3 Kit (Invitrogen, AM1348) and purified using the RNeasy mini-kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Systems Biology 2019Quote: ... 130 µL of CSF from each of the 40 individual CSF samples was incubated with equal volume (130µL) of High Select Top14 Abundant Protein Depletion Resin (Thermo Scientific, A36372) at room temperature in centrifuge columns (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were differentiated into pro-inflammatory (Mφ1) or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
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bioRxiv - Immunology 2023Quote: The plasmacytoma cell line X38-Ag8 producing GM-CSF was cultivated at 37 °C and 5 % CO2 in IMDM (Life technologies) supplemented with 5% FCS (Biowest) ...
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 M guanidine hydrochloride (GuHCl) was obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2019Quote: ... Then the Streptavidin M-280 beads (Thermo Fisher Scientific, 11205D) was added to the protein-RNA mix and incubated for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 μl of magnetic beads (Dynabeads M-280 Tosylactivated, Invitrogen) coupled with Rabbit Gamma globulin antibodies (Jackson Immuno Research Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Immune complexes were collected using Dynabeads M-280 Streptavidin (ThermoFisher) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Streptavidin-coupled magnetic beads (Dynabeads M-280 Streptavidin, ThermoFisher Scientific) were washed (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... Streptavidin coated magnetic beads (Dynabeads M-280, Thermo Fisher Scientific) were pre-equilibrated in binding buffer with 0.25% (w/v ...
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bioRxiv - Genomics 2020Quote: ... 20U/μl M-MuLV (all from Thermo Fisher Scientific, USA), 4μl dNTP mix (dATP ...
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bioRxiv - Developmental Biology 2019Quote: ... and 100 U M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA). To validate the RNA-seq data ...