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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Leptin SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Leptin levels were determined using Human Leptin Instant ELISA kit (Invitrogen BMS2039INST) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 0.5 µl of SAP (Affymetrix), 0.1 µl E ...
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bioRxiv - Genetics 2021Quote: ... Subsequent amplicons were Exo-SAP treated (Exo I, NEB Cat # M0293S and SAP, Affymetrix Cat # 78390) and Sanger sequenced.
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bioRxiv - Physiology 2022Quote: Leptin (Invitrogen, cat no KMC2281), insulin-like growth factor-1 (IGF1 ...
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bioRxiv - Neuroscience 2022Quote: ... Then Shrimp Alkaline Phosphatase (SAP) reaction (total volume 10 µl) was performed with 10× SAP buffer (Affymetrix), 0.5 µl of SAP (Affymetrix) ...
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bioRxiv - Developmental Biology 2022Quote: ... purified by Exo-Sap-IT (Affymetrix, 78201) to remove unincorporated primer ...
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bioRxiv - Microbiology 2019Quote: ... DNA was treated with Exo-SAP-IT (ThermoFisher) and incubated at 37°C for 15 min followed by a 15 min incubation at 80°C to inactivate the enzyme ...
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bioRxiv - Plant Biology 2021Quote: Xylem sap protein concentrations were determined with Pierce® Coomassie Plus kit (Thermo Fisher Scientific), which is based on the Bradford method (Bradford ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... leptin (Cat#: PA5-47023, Thermo Fisher Scientific) and LepR (Cat# ...
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bioRxiv - Genomics 2020Quote: ... with excess primers removed with EXO-SAP-IT (Affymetrix), and then amplified samples were diluted 1/5 in DNA suspension buffer ...
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bioRxiv - Physiology 2021Quote: ... Frozen aliquots were thawed on ice and analyzed using a Leptin Rat ELISA kit (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: ... The PCR products were purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis (through Genewiz) ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was purified using EXO-SAP enzyme (ThermoFisher) and sent for Sanger sequencing analysis ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... or leptin receptor (Cat#: PA1-053, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2019Quote: ... amplicons were cleaned with Exo-SAP (Affymetrix, Santa Clara, CA, USA), quantified with QuantiFluor dsDNA system (Promega ...
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bioRxiv - Genomics 2023Quote: ... 1 ul of EXO-SAP-IT Express (Life Technologies-75001.1.ML) was added to the reaction ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of EXO-SAP (100 U shrimp alkaline phosphatase (Thermo Scientific), 100 U Exonuclease I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplicons were cleaned using Exo-SAP-IT (Affymetrix, Santa Clara, CA, USA), and Sanger-sequenced at the Core Genomic Facility at the University of Sheffield ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products were treated with Exo-Sap II (Thermo Fisher Scientific), and sequence termination reactions were performed from both directions of strands using BigDye Terminator v3.2 (Applied Biosystem) ...
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies against leptin (Cat#: PA5-47023, Thermo Fisher Scientific), LRP1 (Cat# ...
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bioRxiv - Physiology 2019Quote: Antibodies against human adiponectin and leptin were purchased from Thermo Fisher (Adiponectin Monoclonal Antibody (19F1); Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... obese CM was pre-incubated with a leptin neutralizing antibody (Lep ab, 13.3µg/mL, Fisher Scientific #AF398) for 1 hour at 4°C and then cells were treated with lean or obese CM alone or obese CM + Lep ab ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a 1:100 dilution of leptin receptor antibody (cat#PA1-053, ThermoFisher Scientific) in blocking buffer ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Cell Biology 2020Quote: ... Following leptin or vehicle treatment surface BTX-SUR1 was labeled with 1 μg/ml Alexa Fluor® 555 α-bungarotoxin (555-BTX, Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2023Quote: DNA assay kit QuantiT dsDNA HS kit (Invitrogen) was used to quantify the DNA content from cell lysate according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Molecular Biology 2023Quote: ... kit and a TURBO DNA-free Kit (Thermo Fisher Scientific), the RNA was then further purified using RNeasy kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Kit (ThermoFisher Scientific) and subsequently 10 clones per cell line were sequenced (LightRun GATC Carlo Erba) ...
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bioRxiv - Genetics 2020Quote: ... coli kit (Invitrogen). The transfection was performed according to the manufacturer’s guidelines and vector assembly was tested by Sanger sequencing.
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bioRxiv - Systems Biology 2022Quote: A kit (Ambion Magmax™-96 total RNA isolation kit ...
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bioRxiv - Physiology 2023Quote: Transcription Kit (Invitrogen). The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: Kit(Ambion AM1340M). They were injected at the one-cell stage together with the microspheres:
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bioRxiv - Molecular Biology 2021Quote: ... PCR cloning kits from Thermo Scientific (TOPO TA Cloning Kit 450030) and New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Genetics 2021Quote: A commercially available kit (Click-iT EdU Cell Proliferation Kit, ThermoFisher) was used to label proliferating cells with thymidine analog 5-ethynyl-2’- deoxyuridine (EdU) ...