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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Lassa Fever Virus GP2 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Microbiology 2021Quote: ... The Yellow fever NS5 protein was codon-optimised using the algorithms provided by Invitrogen (Thermo Fisher, USA), and a FLAG-tag encoding sequence was added to the carboxyl-terminus ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... and GP2-293 cells were cultured in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... into GP2-293 cells using Lipofectamin 3000 (Invitrogen L3000015). Virus supernatant was collected at both 48 hpt and 72 hpt and further concentrated with Lenti-X concentrator (Clotech 631232) ...
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M280 Sheep anti-mouse IgG or Dynabeads Protein G (Thermo Fisher Scientific) were used for immunoprecipitation ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Physiology 2022Quote: ... sheep (Invitrogen, A21436) and mouse (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-sheep 647 and donkey anti-sheep 488 (Thermo Fisher Scientific). Streptavidin-AMCA was used with donkey anti-mouse biotin for Hu in the adult human colon preparations (Chen et al ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc (18-615) protein expressed in Expi293F (Life Technologies) cells was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256 ...
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bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK-293T and GP2-293 cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Cell Biology 2020Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
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bioRxiv - Microbiology 2019Quote: ... sheep polyclonal rotavirus antiserum (Invitrogen), Alexa Fluor 546-conjugated anti-mouse IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
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bioRxiv - Microbiology 2020Quote: ... 5% sheep’s blood (ThermoFisher, USA), pH 7) ...
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bioRxiv - Microbiology 2022Quote: ... anti-sheep-HRP (A16041, Invitrogen). Antibodies used for immunofluorescence staining are ...
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bioRxiv - Developmental Biology 2023Quote: ... siPROX1 for sheep (Invitrogen; 10620310), or siSOX9 for porcine (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... The secondary antibodies: sheep anti-mouse- and sheep-anti rabbit-HRP were acquired from Invitrogen/Bio-Rad (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... mono-Fc was removed using Pierce Protein A agarose beads (Thermo Scientific). The flow-through containing PvDBP-RII was then mixed with DB1 or DB9 Fab (1.5:1 Fab to protein ratio ...
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bioRxiv - Biochemistry 2023Quote: Codon-optimized sequences encoding full-length phage gp2 homologs were synthesized (Invitrogen/GeneArt) and cloned into UC Berkeley Macrolab vector 2BT (Addgene #29666 ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-sheep (Thermofisher, SA5-10060). Images were obtained by the Odyssey CLx imaging system and analyzed by Image Studio 5.2.5 (LI-COR).
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-sheep (Life Technologies, A11016). For anti-BrdU immunohistochemistry (IHC) ...
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bioRxiv - Microbiology 2021Quote: ... and 5% sheep blood (Life Technologies) and then covered with a cover glass ...
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bioRxiv - Microbiology 2020Quote: ... 5% sheep blood (Thermo Fisher Scientific) and/or 5 μg/ml hemin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... anti-sheep (A11015, Thermo Fisher Scientific), Alexa555-conjugated anti-mouse (A21422 ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-sheep (A21448, ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... or sheep anti-rabbit (Invitrogen: 11203D) beads were added and incubated for 2 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... either sheep anti-mouse (Invitrogen: 11201D) or sheep anti-rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-mouse sPLA2-IIA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...
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bioRxiv - Biochemistry 2023Quote: ... sheep blood cells (Thermo Scientific, Oxoid) were first diluted with 1xPBS ...
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bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... The M25 scFv-Fc was immobilized onto protein A beads (Thermo Fisher Scientific) and cross-linked by dimethyl pimelimidate dihydrochloride (DMP ...
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bioRxiv - Cancer Biology 2022Quote: ... VH-Fc proteins were expressed in the Expi293 expression system (Thermo Fisher Scientific) and purified with protein A resin (GenScript) ...
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bioRxiv - Physiology 2023Quote: ... Fc-FGF-21CT was purified using protein A sepharose 4B (Thermo Fisher Scientific) and dialyzed against PBS ...