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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Acidic organelles in the oocytes were visualized using LysoTracker Red DND-99 and LysoSensor Green DND-189 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... at 100 µg/mL in complete DMEM medium for 4 h and treated with LysoTracker Red DND-99 (Invitrogen #L7528) for 15 min and subsequently imaged on a Nikon CSU-W1 SoRa microscope ...
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bioRxiv - Plant Biology 2022Quote: ... Then samples were ground to an homogenous fine powder and a subsample of 1000 to 2000 μg was precisely weighted in tin capsules to determine the C and N percentages (C% and N%) and the 15N abundance using an elemental analyzer (FLASH 2000 Organic Elemental Analyzer, Thermo Fisher Scientific, Courtaboeuf, France) coupled to an isotope ratio mass spectrometer (delta V isotope ratio mass spectrometer ...
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bioRxiv - Biophysics 2023Quote: For the bead diffusive experiments, Carboxylate-modified FluoroSpheres (715/755 excitation/emission peaks, 97 nm diameter according to manufacturer) (Invitrogen, Thermo Fisher), were diluted 2500 times or 10000 times in purified water ...
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bioRxiv - Cell Biology 2019Quote: ... To monitor for bacterial incorporation into phagolysosomes OCs were further incubated with the acidotrophic probe Lysotracker Red® DND-99 (100 nm, Invitrogen) for 30 min prior to fixation ...
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Genetics 2021Quote: ... worms grown at 25°C were incubated 48 hours prior to imaging with 2 μM LysoTracker Red DND-99 (Life Technologies). Worms were imaged as day 4 adults as described above ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes in live-cell imaging medium and lysosomes were visualized by staining cells with 50nM Lysotracker Red DND-99 (Thermo Fisher) for 30 minutes in live-cell imaging medium according to manufacturer’s description ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell differentiation was induced when cells reached around 90% confluence by replacing the proliferation medium with 99% (v/v) high glucose DMEM (Gibco, UK), 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... Live glands requiring staining were treated for 5 min in a 1:100 dilution of LysoTracker Red DND-99 (Life Technologies) in ice-cold PBS.
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Immunology 2022Quote: Acidic vesicles were visualised by incubating RAW 264.7 cells in medium enriched with LysoTracker™ Red DND-99 (1:2500) (Invitrogen, L7528) for at least 1 hour prior to fixation at 37°C [35] ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... the gut of control or rapamycin-treated flies were dissected out in 4°C chilled PBS and then stained in a 10 μM Lysotracker Red:1μg/ml Hoechst solution (LysoTracker™ Red DND-99, Hoechst 33342 Solution, Thermofisher Scientific) for 3 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
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bioRxiv - Cancer Biology 2024Quote: ... LMD samples were incubated at 99 °C for 30 min followed by 50 °C for 10 min with SMART trypsin (ThermoFisher Scientific). SMART trypsin was added at a ratio of 1 μg per 30 mm2 tissue ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2019Quote: ... The next day, media was replaced by Heavy Lysine (36C6, 15N2 L-Lysine-2HCl), Arginine (13C6, 15N4 L-Arginine-HCl) DMEM (Thermo Fisher Scientific) with 10% dialyzed FBS containing 1ug/ml doxycycline and grown for either 1 day or 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... before cells were treated for 3 hours with nutrients (1 µM insulin plus 0.8 mM L-leucine [Sigma Aldrich catalog numbers I5500 and L8000, respectively] and 0.4mM L-arginine [Acros Organics catalog number 105001000)]) ...
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bioRxiv - Synthetic Biology 2020Quote: ... we used 20 amino acids that contained 2 mM of 13C6 15N2 L-lysine (Thermo Fisher Scientific) and 13C6 15N4 L-arginine (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were washed for 5 min with a wash buffer made with ultra-pure DNase/RNase-Free water (Bio-Lab ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were stained using Akoya Staining Kit for Phenocycler (Akoya Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... heavy isotope (13C-15N-Lysine and 13C-15N-Arginine)-containing DMEM medium (Thermo Fisher Scientific) was added in excess to the cells for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... washed with organoid media and then incubated with 100 cell pellet volumes of organoid media with 10 nM LysoTracker Red DND-99 (Thermo Fisher L7528) at 37C for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... was carried out in 10 mM Tris-HCl – 1 mM EDTA buffer (pH 9) in +99°C for 20 min (PT Module; Thermo Fisher Scientific). Tissue peroxide quenching with 0.9% H2O2 for 15 min was followed by protein blocking with 10% normal goat serum (TBS-NGS ...
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bioRxiv - Immunology 2022Quote: ... Grids were blotted from the backside for 10 seconds at 4℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). We removed one of the blotting arms for one-side blotting because Birbeck granules would be absorbed onto the filter paper ...
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bioRxiv - Biophysics 2022Quote: Lysosomes were stained by loading the cells with LysoTracker™ Red DND-99 (1 mM, ref. L7528) at a final concentration of 50 nM purchased from Life Technologies | Thermo Fisher Scientific (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... the salivary glands were incubated 30 min at RT in Grace’s medium containing 150 nM LysoTracker® Red DND-99 (Molecular Probes L7528), and immediately imaged after incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and ibuprofen (CAS 15687-27-1, purity ≥ 99%), and potassium chloride (KCl, CAS 7447-40-7, purity ≥ 99.7%) were purchased from Thermo Fisher (Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... the plate was again washed six times and 100 μL/well substrate (200 μL 40 mg/mL o-Phenylenediamine dihydrochloride 99+% (OPD, Acros Organics BVBA) + 2 μL H2O2 (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... Dodecane overlay cultivations were performed under the same conditions as controls with no extraction aid but with the addition of 20% of the cultivation volume of sterile dodecane at 99% purity (Acros Organics, UK). All microplate cultivations were made in triplicates to increase the statistical robustness of the results ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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bioRxiv - Systems Biology 2023Quote: ... and 99%) were used to dehydrate the slides and a coverslip was mounted with Diamond anti-fade mounting medium (Invitrogen, cat.no. P36961).
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bioRxiv - Cell Biology 2023Quote: BMDMs were seeded in 96 well plates (6055302, Revvity) and stained with 25 nM LysoTracker DND-99 (LTR) (L7528, Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 1mM N-acetyl-L-cysteine (Thermo Fisher), 100 ng/ml Noggin (Peprotech) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µl of 100 µM bottom splint (IDT) diluted in 7 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The reaction was annealed at 95 °C for 1m following 20 s at 95 °C and decreasing temperature by 1 °C every cycle ...
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bioRxiv - Immunology 2024Quote: ... CSFE labelling of lymphocytes (for which sorting purity was determined to be 97% via flow cytometry) prior to coculturing experiment was done using the CellTrace™ CFSE Cell Proliferation Kit (ThermoFisher Scientific; Cat# C34554) accordingly the manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... CuSO4*5H2O 0.03 g/L and MnSO4*H2O 0.2 g/L) and with NaNO3 (Fisher Scientific Canada, Ottawa, ON, Canada) at various concentrations ...
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bioRxiv - Immunology 2021Quote: ... growth medium including phosphate buffer (5.4 g/l Na2HPO4, 8.6 g/l NaH2PO4·H2O) and 1X Penicillin-Streptomycin (P/S) (Life Technologies 15140122) to avoid bacterial contamination ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lot 183859), ammonium formate (99%, AC401152500), acetonitrile (ACN; Optima®, Lot 184819), and formic acid (99.5%, A117-50) from Fisher Scientific (Waltham, MA) was used for extractions and instrument solvent gradient ...
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bioRxiv - Neuroscience 2021Quote: Zebrafish larvae were incubated for 2 hours with 1:100 LysoTracker™ Green DND-26 or LysoTracker™ Red DND-99 (ThermoFisher Scientific) in E3 medium (final concentration of 10 µM) ...
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bioRxiv - Neuroscience 2023Quote: Fly brains expressing Atg8-mcherry were dissected in Schneinder’s media and stained for 3 minutes with lysotracker (LysoTracker Red DND-99; Thermo Fisher Scientific; 1:2000), rinsed in Schneinder’s media and then directly visualised with a Zeiss Airyscan 880 ...
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bioRxiv - Immunology 2021Quote: ... in 19 L of H2O from a Barnstead Nanopure Water system (Thermo Scientific, Waltham, MA). After incubation with SARS-CoV-2 Spike protein ...
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bioRxiv - Biochemistry 2023Quote: ... blotted from both sides for 3 seconds at 4 ℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA). Images were recorded using a Titan Krios G4 microscope at the University of Tokyo (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: For the staining of lysosomes in living cells Lysotracker Red DND-99 in a final concentration of 50 nM (Thermo Fisher cat. no. L7528) was used according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems, Cat. 4375786). After annealing ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were dried at 70°C for at least 20 h and the total N (% g dry weight [DW]) and 15N (atom%) contents were analyzed using a Flash2000-DELTAplus Advantage ConFlo? System (ThermoFisher Scientific) at Shoko Science Co. ...
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bioRxiv - Cell Biology 2022Quote: ... 25 000 cells/well and MDA-MB-231: 50 000 cells/well) in 96-well Corning™ Stripwell™ Microplates (07-200-97; Fisher Scientific, Pittsburgh, PA, USA), and incubated at 37°C in 5% CO2 for 10 ...