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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The N- and C-terminus of thyA (lpg2868) were amplified by BKMP94-95 and BKMP98-99 primers and introduced into pDonorP1-P4 and pDonorP3-P2 (Invitrogen) to generate pMME1084 and pMME1094 ...
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bioRxiv - Microbiology 2020Quote: Plasmodium 3D7 parasites at 32 hpi (2% parasitemia and 0.5% hematocrit) were incubated in complete media containing 100 μM LysoTracker Red DND-99 (ThermoFisher Scientific) and 20 μM Wortmannin ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were heated to 99 °C for 15 min and aliquots (5 µL) resolved by SDS-PAGE on 8 % – 16 % Tris-glycine minigels (Invitrogen) (150 V ...
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bioRxiv - Cell Biology 2019Quote: Individual RNAs were detected by fluorescence in situ hybridization using ViewRNA Cell Plus (Thermo Fisher Scientific, Cat#88-19000-99) and corresponding RNA probes (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The RNA was then dissolved in a 10μL mixture of 99 μL of DNase/RNase/Nucleotide-free water and 1 μL of SUPERase•In™ (Invitrogen, Life Technologies Incorporated ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... 24h after plating were incubated for 2h in LysoTracker Red DND-99 (75 nM) and for 45 min in Hoechst 33342 (Invitrogen) (0.25μg/ml).
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated under heating from 25 °C to 99 °C in StepOne Plus Real-time PCR system (Thermo Fisher Scientific). The kinetics of fluorescent signals were analyzed to calculate Tm values in Protein Thermal Shift Software 1.3 (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... The sections were then cleared and stained in Clearsee solution with Basic Fuchsin (Fisher scientific Cat no 632-99-5) for 30 minutes followed by two washing steps in Clearsee only ...
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bioRxiv - Molecular Biology 2023Quote: ... immediately followed by a triple MS2 stem-linker sequence (PNAS September 17, 2002 99 (19) 12203-12207) was purchased from ThermoFisher. This was used as a template to make pcDNA3.0_NORAD_NRU78_MS2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Lens apoptosis was determined in the same SAG and control DMSO embryos as heart eye measurements were made by staining with 5 μg/ml Lysotracker Red DND 99 (Invitrogen) for 30 min in the dark as described previously (Ma et al. ...
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bioRxiv - Developmental Biology 2023Quote: Acidic organelles in the oocytes were visualized using LysoTracker Red DND-99 and LysoSensor Green DND-189 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... in H2O] and examining cells by fluorescence microscopy after 20 minutes incubation with LysoTracker (Red DND-99, Thermo Fisher Scientific) used according to manufacturer instructions and 10 minutes fixation in 4% paraformaldehyde in 1xPBS (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... at 100 µg/mL in complete DMEM medium for 4 h and treated with LysoTracker Red DND-99 (Invitrogen #L7528) for 15 min and subsequently imaged on a Nikon CSU-W1 SoRa microscope ...
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bioRxiv - Plant Biology 2020Quote: ... Site directed mutagenesis was used to change serine 328 to alanine (S328A) and aspartic acid (S328D) by quick change PCR (Invitrogen). Clones were sequenced to confirm the changes and absence of mismatches ...
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bioRxiv - Microbiology 2022Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (Thermo Fisher), and 1X non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 1% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco, #15630080) and 1% MEM nonessential amino acids (MEM-NEAA ...
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bioRxiv - Plant Biology 2022Quote: ... Then samples were ground to an homogenous fine powder and a subsample of 1000 to 2000 μg was precisely weighted in tin capsules to determine the C and N percentages (C% and N%) and the 15N abundance using an elemental analyzer (FLASH 2000 Organic Elemental Analyzer, Thermo Fisher Scientific, Courtaboeuf, France) coupled to an isotope ratio mass spectrometer (delta V isotope ratio mass spectrometer ...
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bioRxiv - Biochemistry 2023Quote: ... The dried sample was redissolved in 200 μL of H2O and transferred to a normal vial (CAT# C5000-97, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: For the bead diffusive experiments, Carboxylate-modified FluoroSpheres (715/755 excitation/emission peaks, 97 nm diameter according to manufacturer) (Invitrogen, Thermo Fisher), were diluted 2500 times or 10000 times in purified water ...
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bioRxiv - Cell Biology 2019Quote: ... To monitor for bacterial incorporation into phagolysosomes OCs were further incubated with the acidotrophic probe Lysotracker Red® DND-99 (100 nm, Invitrogen) for 30 min prior to fixation ...
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Genetics 2021Quote: ... worms grown at 25°C were incubated 48 hours prior to imaging with 2 μM LysoTracker Red DND-99 (Life Technologies). Worms were imaged as day 4 adults as described above ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes in live-cell imaging medium and lysosomes were visualized by staining cells with 50nM Lysotracker Red DND-99 (Thermo Fisher) for 30 minutes in live-cell imaging medium according to manufacturer’s description ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell differentiation was induced when cells reached around 90% confluence by replacing the proliferation medium with 99% (v/v) high glucose DMEM (Gibco, UK), 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... Live glands requiring staining were treated for 5 min in a 1:100 dilution of LysoTracker Red DND-99 (Life Technologies) in ice-cold PBS.
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bioRxiv - Immunology 2022Quote: Acidic vesicles were visualised by incubating RAW 264.7 cells in medium enriched with LysoTracker™ Red DND-99 (1:2500) (Invitrogen, L7528) for at least 1 hour prior to fixation at 37°C [35] ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... the gut of control or rapamycin-treated flies were dissected out in 4°C chilled PBS and then stained in a 10 μM Lysotracker Red:1μg/ml Hoechst solution (LysoTracker™ Red DND-99, Hoechst 33342 Solution, Thermofisher Scientific) for 3 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
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bioRxiv - Cancer Biology 2024Quote: ... LMD samples were incubated at 99 °C for 30 min followed by 50 °C for 10 min with SMART trypsin (ThermoFisher Scientific). SMART trypsin was added at a ratio of 1 μg per 30 mm2 tissue ...
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bioRxiv - Microbiology 2023Quote: ... n-tridecanoic acid was from Acros Organics, Geel ...
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bioRxiv - Molecular Biology 2021Quote: ... at N-terminus using Alexa Fluor™ 555 NHS Ester (Invitrogen™: A20009) as per manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... at N-terminus using Alexa Fluor™ 555 NHS Ester (Invitrogen™, A20009) per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Immunology 2024Quote: ... T-allo10 cells or control T-allo Teff cells were labeled with CellTrace carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) for 5 to 8 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were washed for 5 min with a wash buffer made with ultra-pure DNase/RNase-Free water (Bio-Lab ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were stained using Akoya Staining Kit for Phenocycler (Akoya Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... derived from WHO strain MNYC/BZ/62/M379 (Beneke et al., 2017) were grown in M199 medium with Earle’s salts and L-glutamine (Life technologies) supplemented with 26 mM NaHCO3 ...
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bioRxiv - Immunology 2022Quote: ... B cells (0.025×106) were incubated with carboxyfluorescein diacetate succinimidyl ester (CFSE,ThermoFisher, #C34554)-loaded CD8+ T cells (0.225 ×106) ...
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bioRxiv - Neuroscience 2021Quote: ... with the substrate tertiary-butyloxycarbonyl-leucine-methionine-7-amino-4-methylcoumarin (abbreviated to t-BOC-Leu-Met-CMAC, or in short: CMAC) (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific, 17504-044], 1mM L-Glutamine [Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: Naïve T cells were labeled with 5 μM of carboxyfluorescein succinimidyl ester (CFSE, ThermoFisher). Labeled cells were cultured in RPMI 1640 media and supplemented with 10% FBS as well as αCD3/CD28 coated Dynabeads™ (Thermo ...
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bioRxiv - Immunology 2023Quote: CAR-T cells were labeled with 0.2 μM carboxyfluorescein succinimidyl ester (CFSE, Invitrogen, ThermoFisher), washed ...