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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Clusters were co-stained with CellMask Green (Invitrogen; 1:1000) for 5 min before imaging on glass bottom culture plates (MatTek ...
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bioRxiv - Cell Biology 2024Quote: ... TDP-43 protein clusters were visualised using anti-mouse AlexaFluor488 antibody (1:2000, ThermoFisher), incubated for 1 h at RT ...
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bioRxiv - Plant Biology 2020Quote: ... For mitochondrial co-localization roots were stained with MitoTracker Red FM (Invitrogen) t2o8label the mitochondria prior to imaging ...
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bioRxiv - Biophysics 2021Quote: Transforming the chaperone constructs in Escherichia coli DE3 cells (Invitrogen) provided us selected colonies which were then grown in luria broth media with respective antibiotics (ampicillin and carbenicillin 50 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: The DiPRO1-interactions were confirmed with co-immunoprecipitation (Co-IP) using specific antibodies and Protein A Agarose (Thermo Fisher Pierce). Nuclear extracts were isolated as described in the Immunoaffinity purification section ...
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bioRxiv - Microbiology 2019Quote: ... Iron granules (99.98%, ThermoFisher, Germany) and iron coupons (3 cm × 1 cm × 1 mm ...
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bioRxiv - Physiology 2023Quote: ... Mitochondrial proteins were thereafter separated by SDS–PAGE (4–12% Bis-Tris Protein Gels; Invitrogen) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Plant Biology 2022Quote: ... and the co-immunoprecipitated proteins were detected using an anti-GFP antibody (Thermo scientific).
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bioRxiv - Molecular Biology 2020Quote: ... treatment with Proteinase K (20 μg/4 mg mitochondrial protein, Thermo Fisher), or treatment with Proteinase K + 1% Triton X-100 to expose intramitochondrial proteins to protease activity ...
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bioRxiv - Cell Biology 2022Quote: ... nuclear and mitochondrial protein levels were determined by BCA protein assay (Thermo Fisher Scientific, US, 23225). In these experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Iron chemo-sensors Calcein-AM (Invitrogen) and FeRhoNox-1 (Goryo Chemical ...
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bioRxiv - Microbiology 2020Quote: ... Iron granules (99.98% Thermo Fisher, Germany) or iron coupons (3cm × 1cm × 1mm ...
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bioRxiv - Microbiology 2021Quote: ... iron (III) chloride (Acros Organics, 99+%), manganese (II ...
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bioRxiv - Cell Biology 2022Quote: ... and interacting proteins were co-immunoprecipitated with 5 µg of anti-gag antibody per mg of total proteins using Dynabeads protein G (Invitrogen). Proteins were solubilized in Laemmli buffer and separated by SDS–PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Total mitochondrial protein concentrations were determined using the Coomassie Plus kit (Thermo Scientific). Proteins or protein complexes were separated by SDS-PAGE ...
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bioRxiv - Systems Biology 2020Quote: ... whereas mitochondrial proteins were isolated using the Mitochondria Isolation Kit (Thermo Fisher Scientific) according to the instructions provided by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: Co-immunoprecipitation (Co-IP) was performed with Dynabeads™ Protein G Immunoprecipitation Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... mitochondrial membrane potential and mitochondrial mass were measured with TMRE (ThermoFisher) and MTR (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of adult fly mitochondrial preps were determined with a BCA Protein Assay Kit (Thermo Scientific, USA) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration in the mitochondrial pellet was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... total cell protein was extracted and subjected to co-immunopreciptation (Co-IP) with Dynabeads-Protein G (Thermo Fisher Scientific, USA) followed by the protocol described previously (12).
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bioRxiv - Immunology 2022Quote: ... Proteins were co-expressed in Expi293F cells (GIBCO) maintained in Expi293 Expression Media (GIBCO ...
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bioRxiv - Immunology 2023Quote: ... Mitochondrial location of MitoSOX Red was confirmed by co-staining with 500 nM MitoTracker Green (Thermo Fisher Scientific, Fig. A2a). Antimycin A (50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Enriched mitochondrial protein was quantified using Coomassie Plus (Bradford) Assay Kit (Thermo Fisher Scientific) with BSA as a standard.
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial ROS production in relation to mitochondrial mass was detected using 10 μM MitoSOX Red Mitochondrial Superoxide Indicator (Life Technologies) and 150 MTG (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... the final mitochondrial pellet was resuspended in mitochondrial freezing bufRNase inhibitor (Invitrogen)) andfer (200□mM trehalose ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cluster of differentiation 163 [CD163 (Invitrogen PA5-78961 & MA5-16656 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... induced PBMCs (in co-culture with MDA-MB-231) were exposed to MitoSOX Red™ mitochondrial superoxide indicator (Thermo Fisher Scientific) and images were captured at 0 ...
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bioRxiv - Plant Biology 2023Quote: LD-associated proteins were co-immunoprecipitated using the Dynabeads™ Co-immunoprecipitation Kit (Thermo Fisher Scientific) and an anti-CLO1 antibody according to the manufacturer’s protocol for Western Blot ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Dynabeads® co-immunoprecipitation kit (Invitrogen, UK) was used ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of mitochondrial protein was mixed with 1X Native-PAGE sample buffer (Invitrogen BN2003) supplemented with 1% digitonin and incubated on ice for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 7 the mitochondrial membrane potential (MtMP) and cell viability were co-evaluated with the Mitotracker DeepRed and NucGreen respectively (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondrial fraction was separated using a mitochondrial isolation kit (Thermo Fisher Scientific, catalog # 89874). Whole-cell lysates were prepared using RIPA buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Mitochondrial ROS was detected using MitoSOX™ Red Mitochondrial Superoxide Indicator (Thermo Fisher, M36008). Total intracellular ROS was measured using CellROX™ Deep Red Reagent ...
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bioRxiv - Biochemistry 2023Quote: Mitochondrial staining was performed with the mitochondrial probe MitoTracker Red CM-H2XRos (Invitrogen, USA) according to protocols provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Whole-cell protein was harvested using the Pierce Co-Immunoprecipitation (Co-IP) Kit (ThermoFisher Scientific, Waltham, MA) and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet was resuspended in 200 μL of extraction buffer and the mitochondrial protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). For the Proteinase K accessibility assay ...
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bioRxiv - Cancer Biology 2021Quote: The protein–protein interaction for endogenous protein was studied by using the Co-Immunoprecipitation Kit (Thermo Scientific). For overexpressed HA-tag proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial protein (50 µg) was re-suspended in 1X NativePAGE Sample Buffer (Thermo Fisher Scientific, #BN2003) and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.3g/L iron (III) chloride (FeCl3•6H2O, Acros Organics), 21mg/L Zinc chloride (ZnCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... iron(III) chloride anhydrous (I/1035/50, Fisher Scientific), zinc chloride (≥98% ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial superoxide production was measured using the MitoSOX Red mitochondrial superoxide indicator fluorescent probe (Invitrogen). Cells grown and treated with TGFβ and TAK1 inhibitors in 96-well plates were washed twice with PBS and subsequently incubated for 10 min with Mitosox Red (5μM ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial-specific probe MitoSOX Red Mitochondrial Superoxide Indicator (M36008, Thermo Fisher Scientific, Hampton, NA, USA) was used to evaluate mitochondrial superoxide anion ...
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bioRxiv - Neuroscience 2022Quote: Co-immunoprecipitation (co-IP) experiments were performed using the Dynabeads Protein G Immunoprecipitation Kit (#10007D, Thermofisher Scientific, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... and/or crude mitochondrial fractions were normalized for total protein content via BCA Assay (Thermo Scientific 23225). Samples were resolved by SDS-PAGE on Tris-glycine gels (Invitrogen XP04205BOX ...
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bioRxiv - Biochemistry 2022Quote: ... The interaction between B56 subunits and PME-1 was detected by co-immunoprecipitation using anti-HA antibody immobilized on protein G magnetic beads (Invitrogen) to immunoprecipitate PME-1 bound to HA-B56 48 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial and nuclear cellular fractions were performed via Mitochondrial isolation kit (Thermo Fisher Scientific, US, 89874), as previously reported (Stasik et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Intracellular concentrations of mitochondrial ROS were determined using MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen™). Adherent cells were incubated with 5μM MitoSOX Red for 10 min at 37°C ...