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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Inositol polyphosphate 1 phosphatase INPP1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and phosphatase (Thermo Scientific) inhibitor cocktails ...
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bioRxiv - Cancer Biology 2023Quote: ... phosphatase inhibitors (ThermoFisher, 78427). Tissue was incubated on ice for 30 minutes ...
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bioRxiv - Physiology 2020Quote: ... 1% Triton X-100) + Halt protease and phosphatase inhibitor cocktail 100X (100:1; ThermoFisher, MO, USA), and homogenized using a beadmill ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% NP-40 and 1 mM EDTA) containing protease and phosphatase inhibitors (A32955, A32957, Thermo Scientific) to obtain soluble protein fractions ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: The DiFMUP phosphatase assay was performed using the EnzCheck phosphatase assay kit (Invitrogen) and 384-well black transparent bottom plates (Greiner) ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitor mix (Halt™ phosphatase inhibitor cocktail; ThermoFisher Scientific, MA, USA). After gentle stirring on ice for 10 min ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Plant Biology 2022Quote: ... and alkaline phosphatase-conjugated anti-rabbit (# 31340, Thermo Fisher Scientific, 1:5000 dilution). The chemi-luminescence detection was performed with DIG Substrate (Roche ...
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bioRxiv - Genomics 2022Quote: ... containing 1:100 protease inhibitor (Halt™ Protease & Phosphatase Inhibitor Cocktail, Thermo Scientific) and 1:100 0.5M EDTA Solution (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... with 1× protease and phosphatase inhibitor cocktail (ThermoFisher Scientific, Waltham, MA, USA; 78440) to purify synaptoneurosomes ...
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bioRxiv - Physiology 2022Quote: ... 1% IGEPAL CA-60) with 1x Halt protease and phosphatase inhibitors (78440; ThermoFisher) on ice using Dual Kontes glass tissue grinders ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg/ml leupeptin) plus protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific). Cell extracts were stored at −80°C until use ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% Halt™ Protease and Phosphatase Inhibitor Cocktail (78446, Thermo Fisher Scientific). Cells were scraped to obtain the adhered cells and the lysate was collected ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 1 X Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher). After 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... protease and phosphatase inhibitor cocktail (1:100; ThermoFisher Scientific, Waltham, MA; Cat#78444) and recombinant RNasin ribonuclease inhibitor (1 ul/ml ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 1% cocktail of proteinase and phosphatase inhibitor and PMSF (ThermoFisher Scientific). The cell lysates were centrifuged for 2min at 12000rpm at four degrees ...
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bioRxiv - Microbiology 2023Quote: ... 1 µM CaCl2) containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific). The resulting cell lysate was clarified by centrifugation at 20,600 ×g and 4°C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA) containing protease and phosphatase Inhibitor cocktail (Fisher Scientific, Cat No:PI78447) on ice ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1× Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific 78441). Cell lysates were briefly sonicated using a Branson Sonifier 450 (VWR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1:100 HALT Protease and Phosphatase Inhibitors (Cat#:78440 ThermoFisher, Burlington, ON) at a ratio of 750 µL RIPA to 150 mg of tissue ...
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bioRxiv - Bioengineering 2023Quote: ... freshly supplemented with protease and phosphatase inhibitors (1:100, #78441, Thermo Fisher Scientific), incubated 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 1mM EDTA (Sigma Aldrich)] supplemented with 1× Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... 1% SDS) supplemented with a protease and phosphatase inhibitor cocktail (Thermo Scientific, # 78430). The protein concentration of each lysate was measured using the PierceTM BCA Protein Assay Kit (Thermo Scientific #23227 ...
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bioRxiv - Neuroscience 2023Quote: ... or alkaline phosphatase-conjugated goat anti-IgG/IgY (1:1000; A16057; ThermoFisher Scientific) and were incubated for 1-hour in PBS and 0.1% Triton-X 100 at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... with 1 mM PMSF and 1X phosphatase inhibitors (Thermofisher; #36978 and #78427, respectively). Homogenates were spun at 12,000 g for 15 min at 4 °C and pelleted debris were removed ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylation was done using calf intestinal alkaline phosphatase (1 U/μL) (Life Technologies). Each 25-μL 5’ end-labelling reaction contained 10 pmol RNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... supplemented with 1:100 HALT Phosphatase-Protease Inhibitors (Thermo Scientific Catalog number 78441) added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1% (v/v) HALT protease and phosphatase inhibitors (Thermo Scientific 78429) and 125 U/mL Benzonase nuclease (EMD Millipore E1014) ...
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bioRxiv - Neuroscience 2023Quote: ... Upon incubation with alkaline phosphatase-conjugated secondary antibody (Invitrogen #A16014, #A16002; 1:5,000), membranes were exposed to ECF substrate (GE Healthcare #1067873 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% phosphatase inhibitor cocktail 2 (1862495, Thermo Fisher Scientific, Waltham, MA, USA). Lysates of (20-30 μg of protein ...
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bioRxiv - Cell Biology 2024Quote: ... in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen). Cell lysates were cleared by centrifugation at 12,000 rpm for 15 minutes at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were then stimulated with 25 ng/ml EGF (Gibco) for 5 min (other cell lines were not stimulated) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% (v/v ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... HEK293-F cells were cultured in FreeStyle 293 Expression Medium (ThermoFisher). A days before transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfection of HEK293 cells was carried out using Lipofectamine2000 (ThermoFisher) after cells reach a confluency of 70%.
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were cultured with Dulbecco’s Modified Eagles Media (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and COS-7 cells were transfected with Lipofectamine 2000 (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Biochemistry 2020Quote: HEK293T (ATCC) and HEK293 (ATCC) cells were cultured in DMEM (Invitrogen) plus 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 were performed with 4 μL Lipofectamine 2000 (Invitrogen) and 1-2.5 μg plasmid DNA per 35 mm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were maintained in Eagle’s Minimum Essential Medium (EMEM; Gibco) and SH-SY5Y cells in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Immunology 2021Quote: ... HEK293-hCD4 were detached using enzyme-free cell dissociation buffer (Gibco) and resuspended in FACS buffer (PBS containing 5% FBS) ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 (HEK) cells were grown in DMEM Media (Thermo Fisher Scientific) supplemented with 1mM Glutamax (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293-T cells (AccessionID: CRL_3216) were cultured DMEM without phenol (Gibco) with the same conditions for aforementioned supplements ...
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bioRxiv - Molecular Biology 2020Quote: Flp-In T-REx HEK293 (Thermo Fisher Scientific Catalog Number: R78007) cells were kept according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: Cells employed in this study are HEK293 (Gibco 293-H cells), N2a.dd-EGFP (a neuro-2a mouse neuroblastoma cell line developed by us containing a single integrated copy of an EGFP-DHFR[DD] [EGFP-folA dihydrofolate reductase destabilization domain] fusion protein coding cassette originating from a donor plasmid with 1,000 bp long homology arms to the Prnp gene driven by the Prnp promoter (Prnp ...