Labshake search
Citations for Thermo Fisher :
651 - 700 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
-
bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
-
bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
-
bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
-
bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 14,000 RPM for 30 seconds and separated on 8% SDS-PAGE gels followed by western blotting with mouse anti-HA (H9658, Clone HA-7, MilliporeSigma) or anti-PGK1 monoclonal antibodies (AB_2532235, 22C5D8, Invitrogen), and secondary polyclonal goat anti-mouse antibodies conjugated to horseradish peroxidase (115–035-146 ...
-
bioRxiv - Molecular Biology 2020Quote: ... HA-tagged polyQ and Myc-tagged polyA proteins were enriched using Pierce™ Anti-HA Agarose (Thermo Fisher Scientific) and Myc-Trap®_A Kit (Chromotek ...
-
bioRxiv - Microbiology 2024Quote: ... pDEST-FLAG-HA-nsP4 and pDEST-FLAG-HA-capsid vectors were obtained by recombination using Gateway LR clonase (Invitrogen) in the pDEST-FLAG-HA vector according to the manufacturer instructions ...
-
bioRxiv - Microbiology 2021Quote: The neuraminidase (NA) activity of NDV LaSota and rNDV-H5 was determined using the NA-fluor Influenza Neuraminidase Assay kit (Applied Biosystems, CA, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2022Quote: RNA was amplified with influenza-specific primers (Hoffmann et al., 2001) using Invitrogen Superscript III One-Step RT-PCR with Platinum Taq (ThermoFisher Scientific, Waltham, USA). The simultaneous amplification of all influenza segments is based on a one-step RT-PCR method along with primers designed to bind to the conserved 3’ and 5’ ends of the segments ...
-
bioRxiv - Immunology 2022Quote: ... Mice were anesthetized with isofluorane to intranasally inoculate them with 10 – 30 TCID50 mouse-adapted A/H1N1/California/04/2009 influenza in 50 µL Gibco’s reduced-serum minimum essential media (ThermoFisher, 31985-070, Waltham, MA) [26] ...
-
bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... 3ul of anhydrous ACN and 4 ul of TMT reagent (32ug of TMT reporter tag) of each TMT reporter tag (TMT 10 plex kit, Product number: 90110 and A37725., Thermo Fisher Scientific) was added to each sample and incubated at room temperature on a Thermo mixer with a gentle agitation (600 rpm ...
-
bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
-
bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
-
bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
-
bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). According to the manufacturer’s instruction (QIAamp Viral RNA mini kit (Qiagen)) ...
-
bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 genomic RNA was amplified and detected using forward (5’- CGTGTAGTCTTTAATGGTGTTTCC-3’ ...
-
bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
-
bioRxiv - Molecular Biology 2020Quote: ... TaqMan™ Fast Virus 1-Step Master Mix (4444432, Life Technologies) was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556 ...
-
bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
-
bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
-
bioRxiv - Microbiology 2020Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 N gene RNA was amplified using forward (5’-GACCCCAAAATCAGCGAAAT ...
-
bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
-
bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μl of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher), and 9 μl of molecular-grade water per reaction ...
-
bioRxiv - Immunology 2023Quote: ... Taqman Fast Virus 1-step kit (Thermo Fisher; catalog number 4444434) with oligos and probe specific to the N gene of SARS-CoV2 was used for SARS-CoV-2 RNA detection according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat No. 5555532) and a commercially prepared RNAse P primer/probe combination (IDT ...
-
bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
-
bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
-
bioRxiv - Cancer Biology 2021Quote: ... FC-1242, and FC-1245)(Engle et al., 2019) were cultured in 10% FBS (Biowest) and 1% Anti-Anti (Gibco) in DMEM (Gibco) ...
-
bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
-
bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
-
bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
-
bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
-
bioRxiv - Genetics 2021Quote: ... Liver TAGs (Infinity Triglycerides Liquid Stable Reagent; Thermo Scientific) were determined following tissue lipid extraction in chloroform:methanol (2:1) ...
-
bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
-
bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
-
bioRxiv - Immunology 2021Quote: ... anti-V5 tag (clone R960-25, Thermo Fisher Scientific), anti-VSVg tag (rabbit pAb ...
-
bioRxiv - Immunology 2021Quote: ... anti-HSV tag-172Yb (rabbit polyclonal, Thermo Fisher Scientific), anti-S tag-165Ho (clone SBSTAGa ...
-
bioRxiv - Neuroscience 2021Quote: ... a 6XHis tag and SUMO protein expression plasmid (Invitrogen), with a TA cloning site ...
-
bioRxiv - Immunology 2021Quote: ... anti-HSV tag-172Yb (rabbit polyclonal, Thermo Fisher Scientific), anti-S tag-165Ho (clone SBSTAGa ...
-
bioRxiv - Molecular Biology 2021Quote: ... The tag was synthesised by GeneArt (Thermo Fisher Scientific). The targeting construct was generated by Gibson assembly (Gibson Assembly Master Mix kit ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Tandem mass tag (TMT) isobaric reagents (Thermo Fisher Scientific) were resuspended in anhydrous acetonitrile (final concentration of 20 μg/μL) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Biotin Anti-C-tag Conjugate (Thermo Fisher Scientific, 7103252100); insulin receptor β (Cell Signaling Technology ...
-
bioRxiv - Biochemistry 2020Quote: ... rabbit anti-TAP-Tag (Thermo Scientific; dilution 1:10,000), mouse anti-GFP (Sigma ...