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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Influenza Virus Hemagglutinin 1 HA1 H9N2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
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bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
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bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2022Quote: ... The CDC universal Influenza A matrix gene qPCR assay (50) was performed on a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific) using a standard curve to quantify total influenza genome copies per lungs.
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bioRxiv - Immunology 2021Quote: The NA activity of the H11-NA(X) PV was determined via the NA-Fluor™ Influenza Neuraminidase Assay (Life Technologies 4457091) kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: The neuraminidase (NA) activity of NDV LaSota and rNDV-H5 was determined using the NA-fluor Influenza Neuraminidase Assay kit (Applied Biosystems, CA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: RNA was amplified with influenza-specific primers (Hoffmann et al., 2001) using Invitrogen Superscript III One-Step RT-PCR with Platinum Taq (ThermoFisher Scientific, Waltham, USA). The simultaneous amplification of all influenza segments is based on a one-step RT-PCR method along with primers designed to bind to the conserved 3’ and 5’ ends of the segments ...
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bioRxiv - Immunology 2022Quote: ... Mice were anesthetized with isofluorane to intranasally inoculate them with 10 – 30 TCID50 mouse-adapted A/H1N1/California/04/2009 influenza in 50 µL Gibco’s reduced-serum minimum essential media (ThermoFisher, 31985-070, Waltham, MA) [26] ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...