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Citations for Thermo Fisher :
101 - 150 of 8699 citations for IL 7 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Goat anti-mouse and anti-human antibodies pre-coupled to Alexa Fluor 647 (Invitrogen, Rockford, IL, USA) were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... we used 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) or FxCycle™ PI/RNase Staining Solution (Thermofisher ...
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bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
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bioRxiv - Neuroscience 2022Quote: ... 7-aminoactinomycin D (7-AAD, Thermo Fisher, A 1310) was added 1:50 as a cell death marker.
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 (Invitrogen, Massachusetts, USA) were all measured according to kit protocols ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 7-AAD (Invitrogen), Live/dead fixable viability dye (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Each group of mice was killed at 7 weeks of age, and their organs (blood, liver, heart and kidney) were removed and were soaked with RNALater (Thermo Fisher Scientific, Rockford, IL, USA) before being subjected to RNA extraction ...
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bioRxiv - Immunology 2021Quote: ... IL-1ß (ThermoFisher) 200ng/ml ...
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bioRxiv - Immunology 2021Quote: ... IL-10 (ThermoFisher) was added at 10ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Neuroscience 2021Quote: ... IL-6 and IL-1β using mouse ELISA kits (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... and (Affymetrix eBioscience, for IL-6, IL-18 and TNF-α) in accordance with the manufacturer’s instructions ...