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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for IL 5 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and anti-IL-2-PE (Affymetrix eBioscience ...
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bioRxiv - Immunology 2024Quote: ... mouse IL-1beta (Invitrogen™ 88701388), human IL-8 (Invitrogen™ 88808677) ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the anti-inflammatory cytokine IL-10 were detected using specific mouse IL-1β (Invitrogen, California, USA), IL-6 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were collected and we measured the IL-1β concentrations using IL-1β ELISA kits (Invitrogen) and following manufacturer instructions.
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bioRxiv - Immunology 2023Quote: IL-1β secretion was detected by IL-1 beta Mouse Uncoated ELISA Kit (88-7013-88; Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 min each and then incubated with 1:200 diluted anti-rabbit or mouse IgG-horseradish peroxidise (HRP) (Thermo Scientific, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Neuroscience 2021Quote: ... 0.5% sodium deoxycholate and 0.1% SDS plus protease and phosphatase inhibitor cocktails (Cat. Nos. 78430 and 78428, respectively; Thermo Fisher Scientific, Rockford, IL). Tissue homogenates were sonicated and centrifuged as described in detail in our previous reports (13 ...
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bioRxiv - Immunology 2022Quote: ... IL-5 and IFN-γ were quantified using Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ (Thermo Fisher Scientific, Waltham, MA, USA). IP-10 (CXCL10) ...
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bioRxiv - Microbiology 2020Quote: TaqMan RT-PCR was performed with the N2 primer/probe set and BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific) [31] using the QuantStudio 5 system (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Microbiology 2020Quote: ... and cultured in Eagle’s medium (MEM, Fujifilm Woko Chemicals, Japan) supplemented with 5% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Rockford, IL), 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... After lysis in breaking buffer (20 mM potassium phosphate, 5 mM EDTA, 1 mg mL−1 lysozyme, 1 Pierce Protease Inhibitor Tablet (Thermo Scientific, Rockford, IL), 1 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... Both human and simian hepatocytes were maintained at 37 °C in 5% CO2 in William’s E medium (Gibco) supplemented with 10% fetal clone III serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... at 4% haematocrit in supplemented RPMI media (RPMI-HEPES, 0.2% NaHC03, 5% heat-inactivated human serum [Australian Red Cross], 0.25% AlbumaxII [GIBCO] ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were stimulated for 5 days with Dynabeads Human T-Activator CD3/CD28 (Life Technologies, Carlsbad, CA) at a bead-to-cell ratio of 3:1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were collected for cytokine analysis by ELISA (IL-2 BD-OptEIA™; IL-17A, IFN-γ ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... The membranes were washed with TBST (thrice for 5 minutes each) and developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA). The bands were visualized through ImageQuant LAS500 series (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were then stained with a combination of Mitotracker Green FM ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 ng mL-1 IL-10 (ThermoFisher), and 10 ng mL-1 IL-15 (R&D Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... IL-18 (Thermo Fisher, 88-50618-22), TNF-α (eBioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... and EDTA (Thermo Scientific, Rockford, IL, USA). Cells were then sonicated in a water bath 3 times for 30 seconds each with 30 second rest intervals before being centrifuged at 21,000 × g for 10 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... PE-pro-IL-1β (NJTEN3, ThermoFisher Scientific), FITC-F4/80 (BM8 ...
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bioRxiv - Immunology 2020Quote: ... IL-17A-AlexaFluor488 (clone eBio64Dec17, Thermo Fisher Scientific Cat# 53-7179-42 ...
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bioRxiv - Immunology 2021Quote: ... IL-6 mouse ELISA kit (ThermoFisher Scientific) and IL-1β mouse ELISA kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... IL-1β (Invitrogen; sensitivity 8 pg/mL), and IL-33 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 U/ml IL-2 (Gibco) for 2 days ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-10 (clone JES5-16E3, Thermo Fisher) Mouse T cells (syngeneic MB49 model of urothelial carcinoma) ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-4 was purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... anti-V5 (Thermo Fisher, Rockford, IL, USA) diluted 1:5,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Glutathione beads (Thermo Fisher, Rockford, IL, USA) were used to purify the samples to obtain only Cnnm2 c-tail ...
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bioRxiv - Microbiology 2021Quote: ... and phosphatase inhibitor (Thermo Scientific, Rockford, IL). Protein amounts were determined using the BCA protein assay kit (Thermo Scientific) ...
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bioRxiv - Genetics 2020Quote: ... 50ng/mL IL-4 (Life Technologies PHC0045); for MDMs ...
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bioRxiv - Cancer Biology 2021Quote: ... and EDTA (Thermo Scientific, Rockford, IL, USA). Cells were then sonicated in a water bath for three 30 second increments ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and TNF (eBioscience or Invitrogen) were used ...
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bioRxiv - Immunology 2022Quote: ... IL-6 (Invitrogen; cat. no 88-7064) and TNF-α (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... 50 ng ml−1 IL-21 (Invitrogen), and 1×10^5 cells ml−1 irradiated 3T3-CD40L feeder cells (ARP 12535) ...
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bioRxiv - Immunology 2020Quote: ... and IL-28a/b (Thermo Scientific Mm04204156_gH) and results were normalized to GAPDH (Mm.PT.39a.1 ...
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...